Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,309,016	C→T	*418* (TAG→TAA)	kch ←	voltage‑gated potassium channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,309,016	0	C	T	100.0%	216.7 / NA	67	*418* (TAG→TAA)	kch	voltage‑gated potassium channel
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/34);  total (33/34)

TATCCACAGCATCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTCTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTAAAACTCGCGC  >  NC_000913/1308889‑1309150                                                                                                                                |                                                                                                                                       tATCCACAGCATCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                                             <  1:1613513/139‑1 (MQ=255)     cacaGCATCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGAt                                                                                                                         >  2:1588924/1‑139 (MQ=255)            tCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCg                                                                                                                           <  2:1668430/130‑1 (MQ=255)            tCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGGCTTTAAACTTTATTTTTGCACCg                                                                                                                           >  1:1668430/1‑130 (MQ=255)                    tGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCt                                                                                                          <  1:1588924/139‑1 (MQ=255)                     gCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCtt                                                                                                         >  2:895735/1‑139 (MQ=255)                                     gCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTTAGCTTTCGTTTCTAGtt                                                                                             <  1:1082028/135‑1 (MQ=255)                                     gCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGtt                                                                                             >  2:1082028/1‑135 (MQ=255)                                             cGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGc                                                                                 <  1:1367680/139‑1 (MQ=255)                                                         cGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCAt                                                                     <  1:895735/139‑1 (MQ=255)                                                                      cTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTc                                                                                                       <  1:451161/92‑1 (MQ=255)                                                                      cTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTc                                                                                                       >  2:451161/1‑92 (MQ=255)                                                                      cTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGttt                                                                                                   <  1:705792/96‑1 (MQ=255)                                                                      cTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGttt                                                                                                   >  2:705792/1‑96 (MQ=255)                                                                      cTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTc                                                                                                  <  2:510356/97‑1 (MQ=255)                                                                      cTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTc                                                                                                  >  1:510356/1‑97 (MQ=255)                                                                      cTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGAcc                                                                 <  2:553839/130‑1 (MQ=255)                                                                      cTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGAcc                                                                 >  1:553839/1‑130 (MQ=255)                                                                      cTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaa                                                        >  2:742760/1‑139 (MQ=255)                                                                      cTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaa                                                        <  1:742760/139‑1 (MQ=255)                                                                        aaaTGTACTTGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCTGTCTTTAAACTTTATTTTTgc                                                                                                                               >  2:164510/1‑66 (MQ=255)                                                                        aaaTGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCtt                                                                                                         >  2:847486/1‑88 (MQ=255)                                                                        aaaTGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCtt                                                                                                         <  1:847486/88‑1 (MQ=255)                                                                        aaaTGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGaaa                                                                            >  2:953264/1‑117 (MQ=255)                                                                        aaaTGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGaaa                                                                            <  1:953264/117‑1 (MQ=255)                                                                        aaaTGTACTGGGCACCATTGCGGGGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgc                                                                                                                               <  1:164510/66‑1 (MQ=255)                                                                             tACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGa                                                                              >  2:376175/1‑110 (MQ=255)                                                                             tACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGa                                                                              <  1:376175/110‑1 (MQ=255)                                                                               cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                                             <  2:248014/61‑1 (MQ=255)                                                                               cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                                             >  1:248014/1‑61 (MQ=255)                                                                               cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGt                                                                                                               <  2:434450/75‑1 (MQ=255)                                                                               cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGt                                                                                                               >  1:434450/1‑75 (MQ=255)                                                                               cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTg                                                               <  1:1324736/123‑1 (MQ=255)                                                                               cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTg                                                               >  2:1324736/1‑123 (MQ=255)                                                                               cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaaca                                                      >  2:91071/1‑132 (MQ=255)                                                                               cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaaca                                                      <  1:91071/132‑1 (MQ=255)                                                                               cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGa                                               >  1:676520/1‑139 (MQ=255)                                                                               cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGa                                               >  2:1466471/1‑139 (MQ=255)                                                                                     aCCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                                             >  2:569016/1‑55 (MQ=255)                                                                                     aCCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                                             <  1:569016/55‑1 (MQ=255)                                                                                     aCCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAg                                         <  1:1466471/139‑1 (MQ=255)                                                                                         ttGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCata                                     >  1:848584/1‑139 (MQ=255)                                                                                            cGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                                             >  2:1703434/1‑48 (MQ=255)                                                                                            cGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                                             <  1:1703434/48‑1 (MQ=255)                                                                                            cGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCtt                                                                                                         <  1:532892/68‑1 (MQ=255)                                                                                            cGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCtt                                                                                                         >  2:532892/1‑68 (MQ=255)                                                                                            cGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCAt                                                                     >  2:102124/1‑104 (MQ=255)                                                                                            cGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCAt                                                                     <  1:102124/104‑1 (MQ=255)                                                                                             ggTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCat                                 <  2:848584/139‑1 (MQ=255)                                                                                                    aaTAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTc                                                                                                       >  2:759801/1‑62 (MQ=255)                                                                                                    aaTAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTc                                                                                                       <  1:759801/62‑1 (MQ=255)                                                                                                           ttACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTTAACAACATTGAAACGAGCATATCATTATTAATCTCTTCa                   >  2:969077/1‑139 (MQ=255)                                                                                                            tACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGAc                                                                  >  2:854020/1‑91 (MQ=255)                                                                                                            tACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGAc                                                                  <  1:854020/91‑1 (MQ=255)                                                                                                              ctctTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCa                >  1:218092/1‑139 (MQ=255)                                                                                                                   ccGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATAcc                                                                       <  2:1452419/79‑1 (MQ=255)                                                                                                                   ccGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATAcc                                                                       >  1:1452419/1‑79 (MQ=255)                                                                                                                   ccGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCAttatta                            <  2:1543787/122‑1 (MQ=255)                                                                                                                   ccGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCAttatta                            >  1:1543787/1‑122 (MQ=255)                                                                                                                      tCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGaaaa                                                                           <  2:1122375/72‑1 (MQ=255)                                                                                                                      tCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGaaaa                                                                           >  1:1122375/1‑72 (MQ=255)                                                                                                                      tCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCatat                                    <  2:839194/111‑1 (MQ=255)                                                                                                                      tCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCatat                                    >  1:839194/1‑111 (MQ=255)                                                                                                                        tttAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGt                                                                                              <  2:458627/51‑1 (MQ=255)                                                                                                                        tttAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGt                                                                                              >  1:458627/1‑51 (MQ=255)                                                                                                                            aaCTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTAAAACTCgcgc  <  2:676520/139‑1 (MQ=255)                                                                                                                             aCTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGaaa                                                                            <  2:1186827/64‑1 (MQ=255)                                                                                                                                |                                                                                                                                       TATCCACAGCATCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTCTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTAAAACTCGCGC  >  NC_000913/1308889‑1309150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑