| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,317,517 | A→G | I300T (ATC→ACC) | trpB ← | tryptophan synthase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,317,517 | 0 | A | G | 88.5% | 171.9 / 9.2 | 61 | I300T (ATC→ACC) | trpB | tryptophan synthase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base A (4/3); new base G (27/27); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.11e-01 | |||||||||||
CCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGATGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCGAAATAGATACCCACGCGACCATGTTTTAGCGGTGCGCCGTGCTCGCCAGTTTCGATACCGTGACCACCTGGCTC > NC_000913/1317385‑1317650 | ccTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGt > 1:1694527/1‑139 (MQ=255) gCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGAttc > 1:1633199/1‑139 (MQ=255) gCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGATGGAGTAAGAttc > 2:1296684/1‑139 (MQ=255) gCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTg > 1:731117/1‑139 (MQ=255) gCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTg > 2:1289409/1‑139 (MQ=255) cGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCa < 1:1099964/133‑1 (MQ=255) cGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCa > 2:1099964/1‑133 (MQ=255) tGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTc > 1:1559725/1‑139 (MQ=255) aaGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCtt < 2:1694527/139‑1 (MQ=255) aaGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCtt < 2:1559725/139‑1 (MQ=255) ttCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGAttc > 1:1348037/1‑117 (MQ=255) ttCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGAttc < 2:1348037/117‑1 (MQ=255) cTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGt > 2:857035/1‑123 (MQ=255) cTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGt < 1:857035/123‑1 (MQ=255) catcatCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCgg < 2:938186/139‑1 (MQ=255) tcGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCgg > 1:696115/1‑134 (MQ=255) tcGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCgg < 2:696115/134‑1 (MQ=255) gTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCa < 1:1289409/139‑1 (MQ=255) gTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCa > 2:1355956/1‑91 (MQ=255) gTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCa < 1:1355956/91‑1 (MQ=255) gTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCGAAATAg > 2:860709/1‑139 (MQ=255) gTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCGAAATAg > 2:952698/1‑139 (MQ=255) aTCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGtt < 2:1385762/106‑1 (MQ=255) aTCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGtt > 1:1385762/1‑106 (MQ=255) gcgcGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCGAAATAGATACCCa > 1:1341943/1‑139 (MQ=255) tCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCGAAATAGATACCCACGCGa < 1:952698/139‑1 (MQ=255) gCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCGa > 2:932905/1‑116 (MQ=255) gCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCGa < 1:932905/116‑1 (MQ=255) cgcgTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCt > 1:1294793/1‑73 (MQ=255) cgcgTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCt < 2:1294793/73‑1 (MQ=255) cgTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCa > 2:1400099/1‑96 (MQ=255) cgTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCa < 1:1400099/96‑1 (MQ=255) tgttgtGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCgg < 1:1203765/86‑1 (MQ=255) tgttgtGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCgg > 2:1203765/1‑86 (MQ=255) gttgtGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCGAAATAGATACCCACGCGACCATGTTTTAGCGGTGCGCCGTg < 2:731117/139‑1 (MQ=255) tgtgGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCa < 2:124543/91‑1 (MQ=255) tgtgGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCa > 1:124543/1‑91 (MQ=255) gtgGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCg > 2:189527/1‑95 (MQ=255) gtgGGCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCg < 1:189527/95‑1 (MQ=255) gggCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCgg > 1:1520265/1‑80 (MQ=255) gggCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCgg < 2:1520265/80‑1 (MQ=255) ggCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCGAAATAGATACCCACGCGACCATGTTTTAGCGGTGCGCCGTGCTCGcc < 2:1633199/139‑1 (MQ=255) ggCCGACAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCGAAATAGATACCCACGCGACCATGTTTTAGCGGTGCGCCGTGCTCGcc > 1:1108936/1‑139 (MQ=255) aCAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATa > 1:1317532/1‑84 (MQ=255) aCAGACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATa < 2:1317532/84‑1 (MQ=255) gACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCGAAATAGATACCCACGCGACCATGTTTTAGCGGTGCGCCGTg < 1:1051941/125‑1 (MQ=255) gACGGGAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCGAAATAGATACCCACGCGACCATGTTTTAGCGGTGCGCCGTg > 2:1051941/1‑125 (MQ=255) gggAAATCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCGAAATAGATACCCACGCGACCATGTTTTAGCGGTGCGCCGTGCTCGCCAGTTTCGATAc < 2:102695/139‑1 (MQ=255) aTCCAGTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCGAAATAGATACCCACGCGACCATGTTTTAGCGGTGCGCCGTGCTCGCCAGTTTCGATACCGTGa < 2:1108936/139‑1 (MQ=255) cAGTCCGGCGGAGATGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCGAAATAGATACCCACGCGACCATGTTTTAGCGGTGCGCCGTGCTCGCCAGTTTCGATACCGTGa > 2:311446/1‑136 (MQ=255) cAGTCCGGCGGAGATGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCGAAATAGATACCCACGCGACCATGTTTTAGCGGTGCGCCGTGCTCGCCAGTTTCGATACCGTGa < 1:311446/136‑1 (MQ=255) gTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCttt < 2:53870/64‑1 (MQ=255) gTCCGGCGGAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCttt > 1:53870/1‑64 (MQ=255) ccGGCGGAGATGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCGAAATAGATACCCACGCGACCa < 2:1538790/90‑1 (MQ=255) ccGGCGGAGATGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCGAAATAGATACCCACGCGACCa > 1:1538790/1‑90 (MQ=255) gcggAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCGAAATAGATACCCACGCGACCATGTTTTAGCGGTGCGCCGTGCTCGCCAGTTTCGATACCGTGACCACCTGGCt < 1:1513687/139‑1 (MQ=255) cggAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATAcc < 2:246743/64‑1 (MQ=255) cggAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATAcc > 1:246743/1‑64 (MQ=255) cggAGGTGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCGAAATAGATACCCACGCGACCATGTTTTAGCGGTGCGCCGTGCTCGCCAGTTTCGATACCGTGACCACCTGGCTc > 2:1667586/1‑139 (MQ=255) cggAGATGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCg < 1:928378/65‑1 (MQ=255) cggAGATGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCg > 2:928378/1‑65 (MQ=255) | CCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGATGGAGTAAGATTCTTCAATCTGCCCGTCTTCGGTTTGCATCATCGGCGCTTTCATACCGAAATAGATACCCACGCGACCATGTTTTAGCGGTGCGCCGTGCTCGCCAGTTTCGATACCGTGACCACCTGGCTC > NC_000913/1317385‑1317650 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |