Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,347,450 C→T G488D (GGT→GAT)  rnb ← ribonuclease II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,347,4500CT92.3% 216.3 / 6.6 78G488D (GGT→GAT) rnbribonuclease II
Reads supporting (aligned to +/- strand):  ref base C (4/2);  new base T (39/33);  total (43/35)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CGCTCGGCCATTTGGACAGTGATTTCATCCTGTGGGCGCGTCGCAGTTTCGCCTTTGATAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAG  >  NC_000913/1347312‑1347583
                                                                                                                                          |                                                                                                                                     
cGCTCGGCCATTTGGACAGTGATTTCATCCTGTGGGCGCGTCGCAGTTTCGCCTTTGATAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGAc                                                                                                                                       <  2:1372408/139‑1 (MQ=255)
       ccATTTGGACAGTGATTTCATCCTGTGGGCGCGTCGCAGTTTCGCCTTTGATAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGcc                                                                                                                                >  2:1226048/1‑139 (MQ=255)
            tGGACAGTGATTTCATCCTGTGGGCGCGTCGCAGTTTCGCCTTTGATAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGt                                                                                                                           <  1:14881/139‑1 (MQ=255)
                   tGATTTCATCCTGTGGGCGCGTCGCAGTTTCGCCTTTGATAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGACCGAGGCCAAAGTGAGGAcc                                                                                                                    >  2:622399/1‑139 (MQ=255)
                        tCATCCTGTGGGCGCGTCGCAGTTTCGCCTTTGATAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGtt                                                                                                               <  1:1226048/139‑1 (MQ=255)
                               gtgGGCGCGTCGCAGTTTCGCCTTTGATAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGcc                                                                                                                                >  2:185129/1‑115 (MQ=255)
                               gtgGGCGCGTCGCAGTTTCGCCTTTGATAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGcc                                                                                                                                <  1:185129/115‑1 (MQ=255)
                                  ggCGCGTCGCAGTTTCGCCTTTGATAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAgg                                                                                                                                  <  2:1300586/110‑1 (MQ=255)
                                  ggCGCGTCGCAGTTTCGCCTTTGATAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAgg                                                                                                                                  >  1:1300586/1‑110 (MQ=255)
                                                   ccTTTGATAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGtt                                                                                                               <  2:1054229/112‑1 (MQ=255)
                                                   ccTTTGATAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGACCGAGGcc                                                                                                                                >  1:53981/1‑95 (MQ=255)
                                                   ccTTTGATAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGACCGAGGcc                                                                                                                                <  2:53981/95‑1 (MQ=255)
                                                   ccTTTGATAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGa                                                                                    <  1:1564631/139‑1 (MQ=255)
                                                   ccTTTGATAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGCCGTATGCTTCCAGATCGAGGCCAAAGAGAGGACCGGGtt                                                                                                               >  1:1054229/1‑112 (MQ=255)
                                                        gATAACCGCTTTCGGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGa                                                                                                                         <  1:1621067/97‑1 (MQ=255)
                                                        gATAACCGCTTTCGGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGa                                                                                                                         >  2:1621067/1‑97 (MQ=255)
                                                                   tCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGt                                                                                                                           <  1:1164540/84‑1 (MQ=255)
                                                                   tCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGt                                                                                                                           >  2:1164540/1‑84 (MQ=255)
                                                                   tCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGcg                                                                                >  2:1444350/1‑127 (MQ=255)
                                                                   tCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGcg                                                                                <  1:1444350/127‑1 (MQ=255)
                                                                        agACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGa                                                               >  2:750953/1‑139 (MQ=255)
                                                                           cGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGa                                                            >  1:1517755/1‑139 (MQ=255)
                                                                           cGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGa                                                            <  2:165607/139‑1 (MQ=255)
                                                                                    aTCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAgttg                                                   >  2:1262978/1‑139 (MQ=255)
                                                                                        tGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGc                                                                                 <  1:460911/105‑1 (MQ=255)
                                                                                        tGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGc                                                                                 >  2:460911/1‑105 (MQ=255)
                                                                                        tGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAgttggttg                                               >  2:620603/1‑139 (MQ=255)
                                                                                        tGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTTCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAgttggttg                                               >  2:888620/1‑139 (MQ=255)
                                                                                         gTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGc                                                                                                                                 <  1:418568/56‑1 (MQ=255)
                                                                                         gTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGc                                                                                                                                 >  2:418568/1‑56 (MQ=255)
                                                                                          tCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGAcc                                                                                                                    >  1:882305/1‑68 (MQ=255)
                                                                                          tCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGAcc                                                                                                                    <  2:882305/68‑1 (MQ=255)
                                                                                              cATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGc                                                                                               <  2:1511763/85‑1 (MQ=255)
                                                                                              cATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGc                                                                                               >  1:1511763/1‑85 (MQ=255)
                                                                                                tatTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCa                                       <  2:1517755/139‑1 (MQ=255)
                                                                                                          tCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGtt                                                                                                               <  2:107318/57‑1 (MQ=255)
                                                                                                          tCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGtt                                                                                                               >  1:107318/1‑57 (MQ=255)
                                                                                                          tCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAAcc                                                        <  1:742782/112‑1 (MQ=255)
                                                                                                          tCGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAAcc                                                        >  2:742782/1‑112 (MQ=255)
                                                                                                           cGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGAcc                                                                                                                    <  2:1596358/51‑1 (MQ=255)
                                                                                                           cGGCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGAcc                                                                                                                    >  1:1596358/1‑51 (MQ=255)
                                                                                                            ggCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAgg                                                                                                                                  >  1:1555853/1‑36 (MQ=255)
                                                                                                            ggCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAgg                                                                                                                                  <  2:1555853/36‑1 (MQ=255)
                                                                                                            ggCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGt                                                                                                                           <  2:812733/43‑1 (MQ=255)
                                                                                                            ggCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGt                                                                                                                           >  1:812733/1‑43 (MQ=255)
                                                                                                            ggCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGtt                                                                                                               >  2:483257/1‑55 (MQ=255)
                                                                                                            ggCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGtt                                                                                                               <  1:483257/55‑1 (MQ=255)
                                                                                                            ggCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGcgac                                                                              <  1:1540211/88‑1 (MQ=255)
                                                                                                            ggCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGcgac                                                                              >  2:1540211/1‑88 (MQ=255)
                                                                                                            ggCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCgg                                                                      <  2:767091/96‑1 (MQ=255)
                                                                                                            ggCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCgg                                                                      >  1:767091/1‑96 (MQ=255)
                                                                                                            ggCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAg                                                              >  1:210905/1‑104 (MQ=255)
                                                                                                            ggCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAg                                                              <  2:210905/104‑1 (MQ=255)
                                                                                                            ggCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAgttggttg                                               <  1:956801/119‑1 (MQ=255)
                                                                                                            ggCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAgttggttg                                               >  2:956801/1‑119 (MQ=255)
                                                                                                            ggCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTgc                                              >  1:444576/1‑120 (MQ=255)
                                                                                                            ggCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTgc                                              <  2:444576/120‑1 (MQ=255)
                                                                                                            ggCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCa                           >  1:506745/1‑139 (MQ=255)
                                                                                                            ggCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCa                           >  1:917786/1‑139 (MQ=255)
                                                                                                            ggCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCa                           >  2:1727499/1‑139 (MQ=255)
                                                                                                            ggCGAAGTCCAGGTGGCGTAT‑CTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAg                          >  2:1545821/1‑139 (MQ=255)
                                                                                                             gCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAg                          <  2:917786/139‑1 (MQ=255)
                                                                                                             gCGAAGTCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAg                          <  1:1727499/139‑1 (MQ=255)
                                                                                                                   tCCAGGTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCa                    >  1:538710/1‑139 (MQ=255)
                                                                                                                       ggTGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGaaa                >  1:612092/1‑139 (MQ=255)
                                                                                                                         tGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTc                                   <  1:610719/118‑1 (MQ=255)
                                                                                                                         tGGCGTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTc                                   >  2:610719/1‑118 (MQ=255)
                                                                                                                             gTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAAt                                                                                                     <  1:468449/48‑1 (MQ=255)
                                                                                                                             gTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAAt                                                                                                     >  2:468449/1‑48 (MQ=255)
                                                                                                                             gTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCaa                                                                                             <  2:377513/56‑1 (MQ=255)
                                                                                                                             gTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCaa                                                                                             >  1:377513/1‑56 (MQ=255)
                                                                                                                             gTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAg                   >  2:408234/1‑130 (MQ=255)
                                                                                                                             gTATGCTTCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAg                   <  1:408234/130‑1 (MQ=255)
                                                                                                                             gTATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTcc          >  2:1503871/1‑139 (MQ=255)
                                                                                                                                 gCTTCCAGATCGAGGCCAACGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCg      >  1:1154252/1‑139 (MQ=255)
                                                                                                                                   ttCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTg    <  1:737902/139‑1 (MQ=255)
                                                                                                                                   ttCCAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTg    >  1:1107505/1‑139 (MQ=255)
                                                                                                                                      cAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGGAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAg  >  2:1224654/1‑138 (MQ=255)
                                                                                                                                      cAGATCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAg  <  1:1224654/138‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                     
CGCTCGGCCATTTGGACAGTGATTTCATCCTGTGGGCGCGTCGCAGTTTCGCCTTTGATAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAG  >  NC_000913/1347312‑1347583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑