Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,389,499	C→T	C190C (TGC→TGT)	ycjG →	L‑Ala‑D/L‑Glu epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,389,499	0	C	T	100.0%	206.0 / NA	64	C190C (TGC→TGT)	ycjG	L‑Ala‑D/L‑Glu epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/33);  total (31/33)

CTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAGCAATTTGAA  >  NC_000913/1389371‑1389633                                                                                                                                  |                                                                                                                                       cTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGTAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTgg                                                                                                                              <  2:964532/139‑1 (MQ=255)   gAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGc                                                                                                                             <  1:1402316/139‑1 (MQ=255)           aGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAgg                                                                                                                    >  2:1450716/1‑139 (MQ=255)                gATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTg                                                                                                               >  1:5404/1‑139 (MQ=255)                 aTAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGc                                                                                                              <  1:904560/139‑1 (MQ=255)                 aTAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGc                                                                                                              <  1:974264/139‑1 (MQ=255)                     ccATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATg                                                                                                          >  2:28459/1‑139 (MQ=255)                       aTCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCt                                                                                                        <  1:1154011/139‑1 (MQ=255)                               aGTGAGCGGATGGTGGCAATTCGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGaacaac                                                                                                >  1:1246462/1‑139 (MQ=255)                                  gAGCGGATGGTGGCAATTCGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGc                                                                                             >  1:1454189/1‑139 (MQ=255)                                   aGCGGATGGTGGCAATTCGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGc                                                                                                                   >  2:287731/1‑116 (MQ=255)                                   aGCGGATGGTGGCAATTCGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGc                                                                                                                   <  1:287731/116‑1 (MQ=255)                                   aGCGGATGGTGGCAATTCGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGc                                                                                                         <  1:117095/126‑1 (MQ=255)                                   aGCGGATGGTGGCAATTCGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGc                                                                                                         >  2:117095/1‑126 (MQ=255)                                     cGGATGGTGGCAATTCGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTc                                                                                          <  2:736973/139‑1 (MQ=255)                                     cGGATGGTGGCAATTCGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTc                                                                                          <  2:1454189/139‑1 (MQ=255)                                     cGGATGGTGGCAATTCGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTc                                                                                          <  2:1246462/139‑1 (MQ=255)                                     cGGATGGTGGCAATTCGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTc                                                                                          <  2:481434/139‑1 (MQ=255)                                     cGGATGGTGGCAATTCGCACAGCTGCG‑CCCGATGCGACGCTAATGGTTGATGCAATTGAATCCTGGCGTGCAGAAGGGTTGGCTGCGCGTTGTCAGCAATGGGCGGATTTAGGAGTTGCGATGCTTGAACAACCGCttg                                                                                          <  2:301590/139‑2 (MQ=255)                                              gCAATTCGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAgg                                                                                 >  1:706169/1‑139 (MQ=255)                                                  ttCGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGa                                                                             >  2:1631335/1‑139 (MQ=255)                                                    cGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATg                                                                           >  1:1483986/1‑139 (MQ=255)                                                    cGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATg                                                                           <  2:706169/139‑1 (MQ=255)                                                                      tGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAg                                                                                                                     <  1:1027559/80‑1 (MQ=255)                                                                      tGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAg                                                                                                                     >  2:1027559/1‑80 (MQ=255)                                                                      tGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAAtttt                                                          >  2:1525174/1‑139 (MQ=255)                                                                      tGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAAtttt                                                          >  2:60420/1‑139 (MQ=255)                                                                      tGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAAtttt                                                          >  2:1321504/1‑139 (MQ=255)                                                                      tGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAAtttt                                                          >  2:399741/1‑139 (MQ=255)                                                                      tGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAAtttt                                                          >  1:583973/1‑139 (MQ=255)                                                                               gATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCg                                                 <  1:874185/139‑1 (MQ=255)                                                                                aTCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGAGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGt                                                >  1:790118/1‑139 (MQ=255)                                                                                  cGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTg                                              <  2:638163/139‑1 (MQ=255)                                                                                        tGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGAtt                                        >  2:1649503/1‑139 (MQ=255)                                                                                          cAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGaa                                                                                                    >  1:844402/1‑77 (MQ=255)                                                                                          cAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGaa                                                                                                    <  2:844402/77‑1 (MQ=255)                                                                                            aaTGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGga                                                                 >  2:896939/1‑110 (MQ=255)                                                                                            aaTGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGga                                                                 <  1:896939/110‑1 (MQ=255)                                                                                             aTGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTg                                              >  2:175629/1‑128 (MQ=255)                                                                                             aTGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTg                                              <  1:175629/128‑1 (MQ=255)                                                                                                   ccTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCtgatga                             <  2:1261244/139‑1 (MQ=255)                                                                                                    cTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGaa                            <  1:60420/139‑1 (MQ=255)                                                                                                      ggCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAg                          <  1:1649503/139‑1 (MQ=255)                                                                                                       gCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGt                         >  2:1380786/1‑139 (MQ=255)                                                                                                          tGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATc                                                   <  2:923605/110‑1 (MQ=255)                                                                                                          tGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATc                                                   >  1:923605/1‑110 (MQ=255)                                                                                                           gCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTc                     <  2:1483986/139‑1 (MQ=255)                                                                                                            cAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCtgacaactttcat                      >  2:1469918/1‑127 (MQ=255)                                                                                                            cAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCtgacaactttcat                      <  1:1469918/137‑11 (MQ=255)                                                                                                            cAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCa                    >  1:718927/1‑139 (MQ=255)                                                                                                                      ggcggcGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCa                                                                                   >  1:901369/1‑66 (MQ=255)                                                                                                                      ggcggcGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCa                                                                                   <  2:901369/66‑1 (MQ=255)                                                                                                                      ggcggcGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGa                                                                                <  2:1082214/69‑1 (MQ=255)                                                                                                                      ggcggcGCGTTGTCAGCCATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAgta                                                                                >  1:1082214/1‑67 (MQ=255)                                                                                                                            gcgTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTtgtg                                    <  2:1142445/107‑1 (MQ=255)                                                                                                                            gcgTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTtgtg                                    >  1:1142445/1‑107 (MQ=255)                                                                                                                            gcgTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCtgatga                             >  2:614628/1‑114 (MQ=255)                                                                                                                            gcgTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCtgatga                             <  1:614628/114‑1 (MQ=255)                                                                                                                            gcgTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAGCAATTTg    >  2:1690876/1‑139 (MQ=255)                                                                                                                            gcgTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAGCAATTTg    <  1:1321504/139‑1 (MQ=255)                                                                                                                             cgTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAg                          >  2:179384/1‑116 (MQ=255)                                                                                                                             cgTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAg                          <  1:179384/116‑1 (MQ=255)                                                                                                                             cgTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAGCAATTTGa   <  1:1690876/139‑1 (MQ=255)                                                                                                                              gTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAGCAATTTGaa  <  1:1450716/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                       CTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTG‑CCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAGCAATTTGAA  >  NC_000913/1389371‑1389633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑