Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,419,159 G→A *163* (TAG→TAA) 
S51S (AGC→AGT) 		 sieB →
ydaF ←		 phage superinfection exclusion protein, Rac prophage
uncharacterized protein, Rac prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,419,1590GA100.0% 213.0 / NA 65*163* (TAG→TAA) 
S51S (AGC→AGT) 		sieB
ydaF		phage superinfection exclusion protein, Rac prophage
uncharacterized protein, Rac prophage
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/35);  total (30/35)

ATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGT  >  NC_000913/1419030‑1419286
                                                                                                                                 |                                                                                                                               
aTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACtgtg                                                                                                                        >  1:156381/1‑139 (MQ=255)
  tAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACtgtgtg                                                                                                                      <  2:672411/139‑1 (MQ=255)
   aGAACGGAAGGGCATCATTCAACATCCGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGc                                                                                                                     <  1:335301/139‑1 (MQ=255)
                    ttCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGgcg                                                                                                    <  2:156381/139‑1 (MQ=255)
                        aCATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCa                                                                                                >  1:1179747/1‑139 (MQ=255)
                         cATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCAc                                                                                               <  2:1179747/139‑1 (MQ=255)
                            cAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAg                                                                                            >  1:1100137/1‑139 (MQ=255)
                               agTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGttt                                                                                         >  2:689520/1‑139 (MQ=255)
                               agTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGttt                                                                                         >  1:1536886/1‑139 (MQ=255)
                               agTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGttt                                                                                         <  2:1536886/139‑1 (MQ=255)
                                                  cTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCt                                                                      <  2:631039/139‑1 (MQ=255)
                                                   ttattaTCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCtt                                                                     <  1:72157/139‑1 (MQ=255)
                                                    tattatCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCttt                                                                    <  2:222978/139‑1 (MQ=255)
                                                    tattatCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCttt                                                                    <  1:1476938/139‑1 (MQ=255)
                                                        atCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCAt                                                                                                         <  1:827964/98‑1 (MQ=255)
                                                        atCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCAt                                                                                                         >  2:827964/1‑98 (MQ=255)
                                                        atCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGt                                                                >  2:1713146/1‑139 (MQ=255)
                                                          ctcGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCtt                                                                                                                   <  1:1483764/86‑1 (MQ=255)
                                                          ctcGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCtt                                                                                                                   >  2:1483764/1‑86 (MQ=255)
                                                                aCCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCa                                                        <  1:1713146/139‑1 (MQ=255)
                                                                         tACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTaa                                                                                <  1:211916/106‑1 (MQ=255)
                                                                         tACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTaa                                                                                >  2:211916/1‑106 (MQ=255)
                                                                              ccATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGtt                                          <  2:1100137/139‑1 (MQ=255)
                                                                              ccATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGtt                                          <  2:964888/139‑1 (MQ=255)
                                                                                aTTTTATGAAGTTATTCTGGAACAGCAAGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGTAGTGAACGTTcc                                        >  2:1033847/1‑139 (MQ=255)
                                                                                        aaGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCTTGGt                                >  2:122462/1‑139 (MQ=255)
                                                                                             aTTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCttt                                                                    <  1:700554/98‑1 (MQ=255)
                                                                                             aTTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCttt                                                                    >  2:700554/1‑98 (MQ=255)
                                                                                             aTTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTgggg                           >  2:139295/1‑139 (MQ=255)
                                                                                              ttCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCa                                                   <  1:107629/114‑1 (MQ=255)
                                                                                              ttCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCa                                                   >  2:107629/1‑114 (MQ=255)
                                                                                                   gAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACtgtgtg                                                                                                                      >  2:776003/1‑42 (MQ=255)
                                                                                                   gAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACtgtgtg                                                                                                                      <  1:776003/42‑1 (MQ=255)
                                                                                                     aCAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACAcg                     >  2:1673114/1‑137 (MQ=255)
                                                                                                     aCAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACAcg                     <  1:1673114/137‑1 (MQ=255)
                                                                                                      cagcagGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCATCCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACAcgccg                  <  1:1033847/139‑1 (MQ=255)
                                                                                                        gcagGAGTAGACGTTTTAATCGTTAACTTACtgtgtg                                                                                                                      >  1:1021720/1‑37 (MQ=255)
                                                                                                        gcagGAGTAGACGTTTTAATCGTTAACTTACtgtgtg                                                                                                                      <  2:1021720/37‑1 (MQ=255)
                                                                                                        gcagGAGTAGACGTTTTAATCGTTAACTTACtgtg                                                                                                                        >  1:314611/1‑35 (MQ=255)
                                                                                                        gcagGAGTAGACGTTTTAATCGTTAACTTACtgtg                                                                                                                        <  2:314611/35‑1 (MQ=255)
                                                                                                        gcagGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAaca                       >  1:446419/1‑132 (MQ=255)
                                                                                                        gcagGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAaca                       <  2:446419/132‑1 (MQ=255)
                                                                                                        gcagGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGca                <  1:139295/139‑1 (MQ=255)
                                                                                                         cagGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTTCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCttt                                                                    <  2:1468839/86‑1 (MQ=255)
                                                                                                         cagGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTTCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCttt                                                                    >  1:1468839/1‑86 (MQ=255)
                                                                                                         cagGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCa                                                                                                <  2:1166717/58‑1 (MQ=255)
                                                                                                         cagGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCa                                                                                                >  1:1166717/1‑58 (MQ=255)
                                                                                                         cagGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGta                                                               >  2:1150222/1‑91 (MQ=255)
                                                                                                         cagGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGta                                                               <  1:1150222/91‑1 (MQ=255)
                                                                                                            gAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACAcc            <  1:689520/139‑1 (MQ=255)
                                                                                                             aGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGta                                                               <  2:1574269/87‑1 (MQ=255)
                                                                                                             aGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGta                                                               >  1:1574269/1‑87 (MQ=255)
                                                                                                             aGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCTTGGTTGGGGAACACGCCGCACACCa           <  1:122462/139‑1 (MQ=255)
                                                                                                              gTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCAt                                                                                                         <  1:740935/44‑1 (MQ=255)
                                                                                                              gTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCAt                                                                                                         >  2:740935/1‑44 (MQ=255)
                                                                                                                   cGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGAtt     >  2:762427/1‑139 (MQ=255)
                                                                                                                      tttAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCgt  >  2:512646/1‑139 (MQ=255)
                                                                                                                      tttAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTCTaa                                                                                <  2:104747/61‑1 (MQ=255)
                                                                                                                      tttAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTCTaa                                                                                >  1:104747/1‑61 (MQ=255)
                                                                                                                       ttAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGgcg                                                                                                    <  1:1538603/40‑1 (MQ=255)
                                                                                                                       ttAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGgcg                                                                                                    >  2:1538603/1‑40 (MQ=255)
                                                                                                                       ttAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGtt                                                                           >  2:1711923/1‑65 (MQ=255)
                                                                                                                       ttAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGtt                                                                           <  1:1711923/65‑1 (MQ=255)
                                                                                                                              ttAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGta                                                               >  2:203267/1‑70 (MQ=255)
                                                                                                                              ttAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGta                                                               <  1:203267/70‑1 (MQ=255)
                                                                                                                                 |                                                                                                                               
ATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGT  >  NC_000913/1419030‑1419286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑