Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,627,124	T→C	D86G (GAT→GGT)	dcp ←	dipeptidyl carboxypeptidase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,627,124	0	T	C	100.0%	257.0 / NA	69	D86G (GAT→GGT)	dcp	dipeptidyl carboxypeptidase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/33);  total (36/33)

CCCAGGGATTCACGGCGCTGCCAGACAGCATCTACCCGCGCGAATAATTCACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCATCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATAGTATTGTTGAAATCAGGCATTTGCGGGTTAAGCGCGATGGCAG  >  NC_000913/1627001‑1627250                                                                                                                            |                                                                                                                               cccAGGGATTCACGGCGCTGCCAGACAGCATCTACCCGCGCGAATAATTCACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGcgccg                                                                                                                 >  1:1292016/1‑139 (MQ=255)             cGGCGCTGCCAGACAGCATCTACCCGCGCGAATAATTCACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCaa                                                                                                     >  2:1535753/1‑139 (MQ=255)                      cAGACAGCATCTACCCGCGCGAATAATTCACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGc                                                                                            <  2:1292016/139‑1 (MQ=255)                          cAGCATCTACCCGCGCGAATAATTCACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGt                                                                                                                         <  1:690458/106‑1 (MQ=255)                          cAGCATCTACCCGCGCGAATAATTCACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGt                                                                                                                         >  2:690458/1‑106 (MQ=255)                                                      gTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGt                                                            >  1:420664/1‑139 (MQ=255)                                                        tCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAAtt                                                                       <  1:736229/126‑1 (MQ=255)                                                        tCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAAtt                                                                       >  2:736229/1‑126 (MQ=255)                                                              agataTCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGc                                                    <  2:648779/139‑1 (MQ=255)                                                               gataTCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGc                                                    <  1:1152638/138‑1 (MQ=255)                                                               gataTCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGc                                                    >  2:1152638/1‑138 (MQ=255)                                                                 tatCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAg                                                 <  2:420664/139‑1 (MQ=255)                                                                  atCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGa                                                                                               <  1:250664/92‑1 (MQ=255)                                                                  atCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGa                                                                                               >  2:250664/1‑92 (MQ=255)                                                                      ttAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGa                                                                                               >  2:1451028/1‑88 (MQ=255)                                                                      ttAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGa                                                                                               <  1:1451028/88‑1 (MQ=255)                                                                      ttAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAg                                                       <  2:1304705/128‑1 (MQ=255)                                                                      ttAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAg                                                       >  1:1304705/1‑128 (MQ=255)                                                                      ttAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATAg                                            >  1:533867/1‑139 (MQ=255)                                                                       tAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTAt                                                                                                                       >  1:637457/1‑63 (MQ=255)                                                                       tAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTAt                                                                                                                       <  2:637457/63‑1 (MQ=255)                                                                         gCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGc                                                    <  2:94949/128‑1 (MQ=255)                                                                         gCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGc                                                    >  1:94949/1‑128 (MQ=255)                                                                          ccAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCaaaa                                                                                                   <  1:158020/80‑1 (MQ=255)                                                                          ccAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCaaaa                                                                                                   >  2:158020/1‑80 (MQ=255)                                                                          ccAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATAGTAtt                                        >  1:46432/1‑139 (MQ=255)                                                                          ccAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATAGTAtt                                        >  1:1228562/1‑139 (MQ=255)                                                                          ccAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATAGTAtt                                        >  2:1634604/1‑139 (MQ=255)                                                                              ttCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGt                                                                                                                         <  1:1707184/54‑1 (MQ=255)                                                                              ttCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGt                                                                                                                         >  2:1707184/1‑54 (MQ=255)                                                                              ttCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTa                                                                                                                        >  2:1707328/1‑55 (MQ=255)                                                                              ttCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTa                                                                                                                        >  2:1350157/1‑55 (MQ=255)                                                                              ttCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTa                                                                                                                        <  1:1350157/55‑1 (MQ=255)                                                                              ttCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTa                                                                                                                        <  1:1707328/55‑1 (MQ=255)                                                                              ttCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGc                                                                                            <  1:665674/83‑1 (MQ=255)                                                                              ttCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGc                                                                                            >  2:665674/1‑83 (MQ=255)                                                                              ttCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAAcgc                                                                                   >  1:871781/1‑92 (MQ=255)                                                                              ttCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAAcgc                                                                                   <  2:871781/92‑1 (MQ=255)                                                                              ttCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTcc                                                         >  1:754327/1‑118 (MQ=255)                                                                              ttCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTcc                                                         <  2:754327/118‑1 (MQ=255)                                                                              ttCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAg                                                       >  1:1565489/1‑120 (MQ=255)                                                                              ttCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAg                                                       <  2:1565489/120‑1 (MQ=255)                                                                              ttCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATAGTAttgtt                                     <  1:1486585/138‑1 (MQ=255)                                                                              ttCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATAGTAttgtt                                     >  2:1486585/1‑138 (MQ=255)                                                                                   ccAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGa                                                                                               >  2:981939/1‑75 (MQ=255)                                                                                   ccAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGa                                                                                               >  2:1311214/1‑75 (MQ=255)                                                                                   ccAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGa                                                                                               <  1:981939/75‑1 (MQ=255)                                                                                   ccAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGa                                                                                               <  1:1311214/75‑1 (MQ=255)                                                                                   ccAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGc                                                    >  1:898901/1‑118 (MQ=255)                                                                                   ccAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGc                                                    <  2:898901/118‑1 (MQ=255)                                                                                   ccAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATAGTATTGTTGAAATc                               >  1:831131/1‑139 (MQ=255)                                                                                     aGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATAGTATTGTTGAAATCAg                             <  1:1535753/139‑1 (MQ=255)                                                                                      gTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTc                                                          >  2:1601417/1‑109 (MQ=255)                                                                                      gTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTc                                                          <  1:1601417/109‑1 (MQ=255)                                                                                      gTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATAGTATTGTTGAAATCAgg                            <  2:831131/139‑1 (MQ=255)                                                                                       ttCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCaaaa                                                                                                   >  1:1117279/1‑67 (MQ=255)                                                                                       ttCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCaaaa                                                                                                   <  2:1117279/67‑1 (MQ=255)                                                                                       ttCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGa                                                                                               >  2:482162/1‑71 (MQ=255)                                                                                       ttCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGa                                                                                               <  1:482162/71‑1 (MQ=255)                                                                                       ttCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATtctc                                                                    <  2:104280/98‑1 (MQ=255)                                                                                       ttCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATtctc                                                                    >  1:104280/1‑98 (MQ=255)                                                                                            cGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCaaaa                                                                                                   <  1:875895/62‑1 (MQ=255)                                                                                            cGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCaaaa                                                                                                   >  2:875895/1‑62 (MQ=255)                                                                                            cGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATAGTATTGTTGAAATCAGGCATTTg                      <  1:1642666/139‑1 (MQ=255)                                                                                                      cTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATa                                             >  2:1316841/1‑106 (MQ=255)                                                                                                      cTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATa                                             <  1:1316841/106‑1 (MQ=255)                                                                                                                cGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATAGTATTGTTGAAATCAGGCATTTGCGGGTTAAGCGCGATGgcag  >  2:986682/1‑139 (MQ=255)                                                                                                                     tAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATtctc                                                                    <  1:86955/68‑1 (MQ=255)                                                                                                                     tAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATtctc                                                                    >  2:86955/1‑68 (MQ=255)                                                                                                                            |                                                                                                                               CCCAGGGATTCACGGCGCTGCCAGACAGCATCTACCCGCGCGAATAATTCACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCATCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATAGTATTGTTGAAATCAGGCATTTGCGGGTTAAGCGCGATGGCAG  >  NC_000913/1627001‑1627250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑