Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,670,581	G→A	G295R (GGA→AGA)	ynfM →	putative arabinose efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,670,581	0	G	A	100.0%	191.1 / NA	60	G295R (GGA→AGA)	ynfM	putative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/28);  total (32/28)

ATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTCAGCAGGA  >  NC_000913/1670449‑1670715                                                                                                                                      |                                                                                                                                       aTGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCa                                                                                                                                  >  1:1611681/1‑139 (MQ=255)          ttcgtCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGaccacc                                                                                                                          <  1:1501351/139‑1 (MQ=255)               cACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTAt                                                                                                                    >  1:41080/1‑139 (MQ=255)                 cGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATgg                                                                                                                  <  2:1611681/139‑1 (MQ=255)                  gCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATggg                                                                                                                 >  2:769392/1‑139 (MQ=255)                    tGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               <  2:986322/139‑1 (MQ=255)                    tGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               <  1:910894/139‑1 (MQ=255)                                tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGac                                                                                                                              <  2:598706/112‑1 (MQ=255)                                tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGac                                                                                                                              >  1:598706/1‑112 (MQ=255)                                tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               >  2:668689/1‑127 (MQ=255)                                tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               <  1:668689/127‑1 (MQ=255)                                tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAg                                                                                                       >  2:1192340/1‑135 (MQ=255)                                tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAg                                                                                                       <  1:1192340/135‑1 (MQ=255)                                tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAt                                                                                                   >  2:1582915/1‑139 (MQ=255)                                   gCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtgtt                                                                                                >  2:1188904/1‑139 (MQ=255)                                    cTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtgttg                                                                                               >  2:281707/1‑139 (MQ=255)                                             gATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACg                                                                                      >  2:1299452/1‑139 (MQ=255)                                              aTGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACgg                                                                                     >  1:1290964/1‑139 (MQ=255)                                              aTGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACgg                                                                                     <  1:769392/139‑1 (MQ=255)                                                    tcCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               >  1:1440088/1‑107 (MQ=255)                                                    tcCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               <  2:1440088/107‑1 (MQ=255)                                                     cccccTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTAt                                                                              >  2:872234/1‑139 (MQ=255)                                                        ccTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               >  1:1207007/1‑103 (MQ=255)                                                        ccTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               <  2:1207007/103‑1 (MQ=255)                                                           ggCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGtt                                                                        >  1:1128110/1‑139 (MQ=255)                                                            gCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGttt                                                                       >  1:1683231/1‑139 (MQ=255)                                                                gtcagtcaGGCCGTGGTTGGCCTATTATCGCT‑GGCCTATTTGACCGGTACATGCAGCTCACCCAAAGCCAGGACCATGACCACCCGCTATGAGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGtt                                                                   >  2:762698/1‑139 (MQ=255)                                                                  cagtcagGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtg                                                                                                  <  2:315586/106‑1 (MQ=255)                                                                  cagtcagGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtg                                                                                                  >  1:315586/1‑106 (MQ=255)                                                                      cagGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                             <  2:41080/139‑1 (MQ=255)                                                                         gCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               <  1:377221/86‑1 (MQ=255)                                                                         gCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               >  2:377221/1‑86 (MQ=255)                                                                              ggTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTAt                                                                                                                    <  2:1065881/76‑1 (MQ=255)                                                                              ggTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTAt                                                                                                                    >  1:1065881/1‑76 (MQ=255)                                                                                   gCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               >  2:1412925/1‑76 (MQ=255)                                                                                   gCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               <  1:1412925/76‑1 (MQ=255)                                                                                   gCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAg                                                <  2:1290964/139‑1 (MQ=255)                                                                                   gCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTAGGCTGTTTGGTTTACTGATGACCTTATTctg                                                >  2:1507622/1‑137 (MQ=255)                                                                                       attatCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGac                                                                                                                              <  1:214571/57‑1 (MQ=255)                                                                                       attatCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGac                                                                                                                              >  2:214571/1‑57 (MQ=255)                                                                                                  gCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               <  2:362595/62‑1 (MQ=255)                                                                                                  gCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               >  1:362595/1‑62 (MQ=255)                                                                                                   cTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAg                                                                                                       >  2:1009186/1‑69 (MQ=255)                                                                                                   cTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAg                                                                                                       <  1:1009186/69‑1 (MQ=255)                                                                                                   cTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                             <  2:979211/111‑1 (MQ=255)                                                                                                   cTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                             >  1:979211/1‑111 (MQ=255)                                                                                                       tttGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCttt                             >  1:1059673/1‑139 (MQ=255)                                                                                                         tGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGc                           <  1:872234/139‑1 (MQ=255)                                                                                                         tGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGc                           <  1:1344127/139‑1 (MQ=255)                                                                                                                     ggAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTa                                                     >  1:1412150/1‑101 (MQ=255)                                                                                                                     ggAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTa                                                     <  2:1412150/101‑1 (MQ=255)                                                                                                                         cTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               >  2:505937/1‑39 (MQ=255)                                                                                                                         cTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               <  1:505937/39‑1 (MQ=255)                                                                                                                          tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               >  2:772631/1‑38 (MQ=255)                                                                                                                          tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               <  1:772631/38‑1 (MQ=255)                                                                                                                          tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtcttct          >  1:57475/1‑139 (MQ=255)                                                                                                                                  aGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTCAGCAGGa  <  1:281707/139‑1 (MQ=255)                                                                                                                                  aGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTCAGCAGGa  <  2:1059673/139‑1 (MQ=255)                                                                                                                                   gCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTa                 >  1:266029/1‑123 (MQ=255)                                                                                                                                   gCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTa                 <  2:266029/123‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       ATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCT‑GGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTCAGCAGGA  >  NC_000913/1670449‑1670715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑