Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,673,314	A→G	L63P (CTG→CCG)	mdtJ ←	multidrug efflux system transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,673,314	0	A	G	100.0%	224.6 / NA	62	L63P (CTG→CCG)	mdtJ	multidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/30);  total (32/30)

ACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCG  >  NC_000913/1673181‑1673446                                                                                                                                      |                                                                                                                                     aCGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCa                                                                                                                                 <  2:1249480/139‑1 (MQ=255)        ccTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCa                                                                                                                                 <  1:308656/132‑1 (MQ=255)        ccTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCa                                                                                                                                 >  2:308656/1‑132 (MQ=255)        ccTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTa                                                                                                                          >  2:1724401/1‑139 (MQ=255)        ccTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTa                                                                                                                          >  2:1256665/1‑139 (MQ=255)            aTTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGcc                                                                                                                      >  2:540686/1‑139 (MQ=255)                      cacaATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGAtt                                                                                                            >  1:388047/1‑139 (MQ=255)                           tCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGc                                                                                                                            >  2:1725698/1‑118 (MQ=255)                           tCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGc                                                                                                                            <  1:1725698/118‑1 (MQ=255)                           tCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGAttttttt                                                                                                       >  2:957297/1‑139 (MQ=255)                           tCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGAttttttt                                                                                                       >  1:40749/1‑139 (MQ=255)                             ccGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTaa                                                                                                     <  2:388047/139‑1 (MQ=255)                                     cAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGaaa                                                                                             >  2:1487002/1‑139 (MQ=255)                                       gggTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAaga                                                                                           <  1:1724401/139‑1 (MQ=255)                                            gtTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGaa                                                                                      <  1:1487002/139‑1 (MQ=255)                                             ttAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAagagag                                                                                        >  1:1627048/1‑136 (MQ=255)                                             ttAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAagagag                                                                                        <  2:1627048/136‑1 (MQ=255)                                             ttAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCAAAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGaaa                                                                                     >  1:944369/1‑139 (MQ=255)                                                  ccGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAagaga                                                                                         >  2:1487917/1‑130 (MQ=255)                                                  ccGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAagaga                                                                                         <  1:1487917/130‑1 (MQ=255)                                                  ccGGCAATTTTCATCAGCGATAAACTTTCGTCAAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAatatat                                                                                >  1:224433/1‑139 (MQ=255)                                                      cAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGa                                                                            <  1:957297/139‑1 (MQ=255)                                                           ttCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACagag                                                                       <  1:613847/139‑1 (MQ=255)                                                             catcaGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGaa                                                                     <  1:1256665/139‑1 (MQ=255)                                                                     aTAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAagaga                                                                                         <  1:598738/111‑1 (MQ=255)                                                                     aTAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAagaga                                                                                         >  2:598738/1‑111 (MQ=255)                                                                      tAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCAcc                                                            <  2:40749/139‑1 (MQ=255)                                                                           tttCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCAt                                                       <  2:143022/139‑1 (MQ=255)                                                                           tttCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCAAAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCAt                                                       <  2:944369/139‑1 (MQ=255)                                                                                                       ggTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTg                           >  1:1206339/1‑139 (MQ=255)                                                                                                            taaataaaATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACg                                                                             >  1:566210/1‑84 (MQ=255)                                                                                                            taaataaaATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACg                                                                             <  2:566210/84‑1 (MQ=255)                                                                                                            taaataaaATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAACATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCt                      >  2:1244147/1‑139 (MQ=255)                                                                                                            taaataaaATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAAAATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCt                      >  2:1242145/1‑139 (MQ=255)                                                                                                               ataaaataCCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGc                   <  1:1556847/139‑1 (MQ=255)                                                                                                                 aaaataCCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGccc                 <  1:1244147/139‑1 (MQ=255)                                                                                                                 aaaataCCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGccc                 <  2:1382710/139‑1 (MQ=255)                                                                                                                   aataCCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCAt               >  2:1404312/1‑139 (MQ=255)                                                                                                                     taCCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGa                                                                                               <  1:1632017/57‑1 (MQ=255)                                                                                                                     taCCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGa                                                                                               >  2:1632017/1‑57 (MQ=255)                                                                                                                          aTACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAaga                                                                                           >  1:1002262/1‑56 (MQ=255)                                                                                                                          aTACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAag                                                                                            <  2:213301/55‑1 (MQ=255)                                                                                                                          aTACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAag                                                                                            >  1:213301/1‑55 (MQ=255)                                                                                                                          aTACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGaa                                                                                      >  1:532749/1‑61 (MQ=255)                                                                                                                          aTACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGaa                                                                                      <  2:532749/61‑1 (MQ=255)                                                                                                                          aTACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGccc                 <  2:1170785/130‑1 (MQ=255)                                                                                                                          aTACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTg        >  1:338398/1‑139 (MQ=255)                                                                                                                          aTACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATCTCCCTCACTGACGCTCGccc                 >  1:1170785/1‑130 (MQ=255)                                                                                                                          aTAACTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAaga                                                                                           <  2:1002262/56‑1 (MQ=39)                                                                                                                                tCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGaaa                                                                                             <  2:158387/48‑1 (MQ=255)                                                                                                                                tCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGaaa                                                                                             >  1:158387/1‑48 (MQ=255)                                                                                                                                tCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGaa                                                                                      <  2:1420398/55‑1 (MQ=255)                                                                                                                                tCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGaa                                                                                      <  2:1357751/55‑1 (MQ=255)                                                                                                                                tCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGaa                                                                                      <  2:518237/55‑1 (MQ=255)                                                                                                                                tCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGaa                                                                                      >  1:518237/1‑55 (MQ=255)                                                                                                                                tCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGaa                                                                                      >  1:1420398/1‑55 (MQ=255)                                                                                                                                tCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGaa                                                                                      >  1:1357751/1‑55 (MQ=255)                                                                                                                                tCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCg  >  1:846702/1‑139 (MQ=255)                                                                                                                                 cccACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAaga                                                                                           <  1:884181/49‑1 (MQ=255)                                                                                                                                 cccACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAaga                                                                                           >  2:884181/1‑49 (MQ=255)                                                                                                                                 cccACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAag                                                                                            >  1:722492/1‑48 (MQ=255)                                                                                                                                 cccACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAag                                                                                            <  2:722492/48‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     ACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCG  >  NC_000913/1673181‑1673446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑