Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,673,789 T→C intergenic (‑288/‑124) mdtJ ← / → tqsA multidrug efflux system transporter/pheromone AI‑2 transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,673,7890TC86.8% 190.4 / 11.2 68intergenic (‑288/‑124)mdtJ/tqsAmultidrug efflux system transporter/pheromone AI‑2 transporter
Reads supporting (aligned to +/- strand):  ref base T (4/5);  new base C (32/27);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 7.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGTATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATGGCAAA  >  NC_000913/1673655‑1673920
                                                                                                                                      |                                                                                                                                   
aTGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCa                                                                                                                                 >  2:863829/1‑139 (MQ=255)
  gAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATa                                                                                                                               >  1:1659167/1‑139 (MQ=255)
            gATGGGTAACTGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAAt                                                                                                                     <  2:1021660/139‑1 (MQ=255)
             aTGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAAtt                                                                                                                    >  2:1699998/1‑139 (MQ=255)
                       gTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGTATCATAGCTTAAGAATTAACTCAAAat                                                                                                          >  2:1435056/1‑139 (MQ=255)
                             aaTAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTc                                                                                                    <  2:1659167/139‑1 (MQ=255)
                               tAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCAc                                                                                                  <  1:863829/139‑1 (MQ=255)
                                aaCGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGTATCATAGCTTAAGAATTAACTCAAAATATTTTCAct                                                                                                 >  1:721043/1‑139 (MQ=255)
                                  cGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGTATCa                                                                                                                                 >  1:662430/1‑105 (MQ=255)
                                  cGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGTATCa                                                                                                                                 <  2:662430/105‑1 (MQ=255)
                                      cTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACt                                                                                                                >  2:738949/1‑118 (MQ=255)
                                      cTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACt                                                                                                                <  1:738949/118‑1 (MQ=255)
                                            aaaTTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGTATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAg                                                                                     <  1:1435056/139‑1 (MQ=255)
                                                tGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAg                                                                                     <  2:86332/135‑1 (MQ=255)
                                                tGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAg                                                                                     >  1:86332/1‑135 (MQ=255)
                                                   ccATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCActtctt                                                                                             <  1:285547/124‑1 (MQ=255)
                                                   ccATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCActtctt                                                                                             >  2:285547/1‑124 (MQ=255)
                                                    cATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGa                                                                             >  2:1515448/1‑139 (MQ=255)
                                                        atatTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGAtttt                                                                         >  2:1037561/1‑139 (MQ=255)
                                                           ttACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGt                                                                      <  1:1699998/139‑1 (MQ=255)
                                                             aCAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGTATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTa                                                                    <  2:721043/139‑1 (MQ=255)
                                                              cAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTat                                                                   <  1:1515448/139‑1 (MQ=255)
                                                                   aTCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCt                                                                                                                            <  1:736194/77‑1 (MQ=255)
                                                                   aTCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCt                                                                                                                            >  2:736194/1‑77 (MQ=255)
                                                                   aTCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTc                                                                                                    <  1:1685965/101‑1 (MQ=255)
                                                                   aTCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTc                                                                                                    >  2:1685965/1‑101 (MQ=255)
                                                                   aTCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTAtgatga                                                              >  2:769817/1‑139 (MQ=255)
                                                                    tCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGAc                                                             >  2:1712828/1‑139 (MQ=255)
                                                                         aGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTa                                                                                                                          <  1:938533/73‑1 (MQ=255)
                                                                         aGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTa                                                                                                                          >  2:938533/1‑73 (MQ=255)
                                                                           tgtTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGGTGACGGAGCGa                                                      >  1:1129319/1‑139 (MQ=255)
                                                                             ttGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGTATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTat                                                                   <  2:1692796/124‑1 (MQ=255)
                                                                             ttGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGTATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTat                                                                   >  1:1692796/1‑124 (MQ=255)
                                                                             ttGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGaaa                                                    >  1:1023112/1‑139 (MQ=255)
                                                                              tGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGt                                                                                 >  2:263145/1‑109 (MQ=255)
                                                                              tGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGt                                                                                 <  1:263145/109‑1 (MQ=255)
                                                                              tGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGaaaa                                                   <  1:1712828/139‑1 (MQ=255)
                                                                                     cGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCAt                                                                                                                                   <  2:485921/52‑1 (MQ=255)
                                                                                     cGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCAt                                                                                                                                   >  1:485921/1‑52 (MQ=255)
                                                                                     cGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTc                                                                                                    <  1:1300067/83‑1 (MQ=255)
                                                                                     cGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTc                                                                                                    >  2:1300067/1‑83 (MQ=255)
                                                                                     cGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACatt                                            >  2:252101/1‑139 (MQ=255)
                                                                                     cGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACa                                              >  1:692803/1‑137 (MQ=255)
                                                                                     cGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACa                                              <  2:692803/137‑1 (MQ=255)
                                                                                         aTCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACattatta                                        <  2:1129319/139‑1 (MQ=255)
                                                                                               gatgatAATTGTCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACATTATTATTAGCa                                  <  1:252101/139‑1 (MQ=255)
                                                                                                          tCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTc                                                                                                    <  2:717168/62‑1 (MQ=255)
                                                                                                          tCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTc                                                                                                    >  1:717168/1‑62 (MQ=255)
                                                                                                          tCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAg                                                         >  1:519786/1‑105 (MQ=255)
                                                                                                          tCCTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAg                                                         <  2:519786/105‑1 (MQ=255)
                                                                                                            cTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTCACTCAAAATAtttt                                                                                                     >  2:1224423/1‑59 (MQ=255)
                                                                                                            cTCGGTAGTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATAtttt                                                                                                     <  1:1224423/59‑1 (MQ=255)
                                                                                                                   gTTGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGAc              <  1:769817/139‑1 (MQ=255)
                                                                                                                    ttGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGAt                                                                            <  1:1318902/76‑1 (MQ=255)
                                                                                                                    ttGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGAt                                                                            >  2:1318902/1‑76 (MQ=255)
                                                                                                                    ttGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACgggg                >  2:713910/1‑136 (MQ=255)
                                                                                                                    ttGAACACGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACgggg                <  1:713910/136‑1 (MQ=255)
                                                                                                                         cacGCCTGATTTGCATCATAGCTTAAGAATTAACTc                                                                                                               <  2:441375/36‑1 (MQ=255)
                                                                                                                         cacGCCTGATTTGCATCATAGCTTAAGAATTAACTc                                                                                                               >  1:441375/1‑36 (MQ=255)
                                                                                                                           cGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACa                                              >  1:172960/1‑99 (MQ=255)
                                                                                                                           cGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACa                                              <  2:172960/99‑1 (MQ=255)
                                                                                                                           cGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATgg      >  1:1710625/1‑139 (MQ=255)
                                                                                                                           cGCCTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATgg      >  2:955369/1‑139 (MQ=255)
                                                                                                                             ccTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATGGCa    >  2:1581768/1‑139 (MQ=255)
                                                                                                                              cTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAAc                    >  2:786186/1‑122 (MQ=255)
                                                                                                                              cTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAAc                    <  1:786186/122‑1 (MQ=255)
                                                                                                                              cTGATTTGCATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACATTATTATTAGAAAAGGAAGAAAAAACGGGGACAAGCATGGCaa   >  2:386023/1‑139 (MQ=255)
                                                                                                                               tGATTTGTATCATAGCTTAAGAATTATCTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATGGCaaa  <  2:1023112/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                   
ATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGTATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATGGCAAA  >  NC_000913/1673655‑1673920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: