Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,709,163 T→C S8P (TCC→CCC)  rsxD → SoxR iron‑sulfur cluster reduction factor component, putative membrane protein of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,709,1630TC95.0% 129.0 / ‑3.8 40S8P (TCC→CCC) rsxDSoxR iron‑sulfur cluster reduction factor component, putative membrane protein of electron transport complex
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (19/19);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCTCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCATCGGT  >  NC_000913/1709035‑1709296
                                                                                                                                |                                                                                                                                     
cGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTta                                                                                                                                >  1:611585/1‑136 (MQ=255)
cGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTta                                                                                                                                <  2:611585/136‑1 (MQ=255)
   tACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCTAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCa                                                                                                                          >  1:267176/1‑139 (MQ=255)
   tACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGGTGCCGCGGCTGTTGCCCGCGCTCAGGACAAAAAAGCCGCCCAGCAGAAGGCTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATAcctg                                                                                                                          >  1:1100998/1‑137 (MQ=255)
       agaagaACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAAc                                                                                                                      <  2:955382/139‑1 (MQ=255)
                   tGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACa                                                                                                          >  1:964579/1‑139 (MQ=255)
                     aTCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCCTAACCAGCGCCAGACATc                                                                                                        <  2:964579/139‑1 (MQ=255)
                      tCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATcg                                                                                                       >  2:1646809/1‑139 (MQ=255)
                       ccGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCTAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATcgc                                                                                                      <  2:267176/139‑1 (MQ=255)
                       ccGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATcgc                                                                                                      <  1:1646809/139‑1 (MQ=255)
                           gcAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCAt                                                                                                  <  2:476329/139‑1 (MQ=255)
                                   gCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGt                                                                                          <  1:1397212/139‑1 (MQ=255)
                                               gCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCg                                                                              >  1:801701/1‑139 (MQ=255)
                                                   ttGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGc                                                                          >  1:733515/1‑139 (MQ=255)
                                                          cgcTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTAt                                                                                               <  2:918158/111‑1 (MQ=255)
                                                          cgcTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTAt                                                                                               >  1:918158/1‑111 (MQ=255)
                                                            cTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAgg                                                                 >  1:978871/1‑139 (MQ=255)
                                                                    aaaaaaGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAg                                                         <  2:733515/139‑1 (MQ=255)
                                                                           ccgccCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACt                                                  <  1:1145941/139‑1 (MQ=255)
                                                                                        ggTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTttggt                                     <  2:978871/139‑1 (MQ=255)
                                                                                           tGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCTCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTttggttgg                                  >  1:819985/1‑139 (MQ=255)
                                                                                             tAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTta                                                                                                                                <  1:1660962/43‑1 (MQ=255)
                                                                                             tAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTta                                                                                                                                >  2:1660962/1‑43 (MQ=255)
                                                                                             tAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTgggg                                >  1:1301042/1‑139 (MQ=255)
                                                                                              aaaCGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTg                                                                                           <  2:1396689/79‑1 (MQ=255)
                                                                                              aaaCGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTg                                                                                           >  1:1396689/1‑79 (MQ=255)
                                                                                                    ggACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTgggg                                >  2:422946/1‑132 (MQ=255)
                                                                                                    ggACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTgggg                                <  1:422946/132‑1 (MQ=255)
                                                                                                    ggACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACtctc                         >  2:260645/1‑139 (MQ=255)
                                                                                                                 ttCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTgggg                                <  2:721090/119‑1 (MQ=255)
                                                                                                                 ttCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTgggg                                >  1:721090/1‑119 (MQ=255)
                                                                                                                  tCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAAt                                                              <  1:842664/88‑1 (MQ=255)
                                                                                                                  tCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAAt                                                              >  2:842664/1‑88 (MQ=255)
                                                                                                                    aGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAg                                                                                                             >  2:494634/1‑39 (MQ=255)
                                                                                                                    aGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAg                                                                                                             <  1:494634/39‑1 (MQ=255)
                                                                                                                       aTAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCa                                                                                                   >  2:668107/1‑46 (MQ=255)
                                                                                                                       aTAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCa                                                                                                   <  1:668107/46‑1 (MQ=255)
                                                                                                                        tagctagcTCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCATc     <  2:819985/139‑1 (MQ=255)
                                                                                                                        tagctagcCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATcc               >  2:509915/1‑129 (MQ=255)
                                                                                                                        tagctagcCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATcc               <  1:509915/129‑1 (MQ=255)
                                                                                                                           ctagccCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtttt                                          <  1:1311917/99‑1 (MQ=255)
                                                                                                                           ctagccCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtttt                                          >  2:1311917/1‑99 (MQ=255)
                                                                                                                           ctagccCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCATCGGt  >  2:1110985/1‑139 (MQ=255)
                                                                                                                                |                                                                                                                                     
CGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCTCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCATCGGT  >  NC_000913/1709035‑1709296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: