Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,781,964	C→G	G190G (GGC→GGG)	ydiS →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,781,964	0	C	G	100.0%	215.4 / NA	59	G190G (GGC→GGG)	ydiS	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (29/30);  total (29/30)

GGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCC  >  NC_000913/1781834‑1782090                                                                                                                                   |                                                                                                                               gggATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACAcc                                                                                                                        >  1:711346/1‑139 (MQ=255)  ggATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCa                                                                                                                       >  2:1568520/1‑139 (MQ=255)                 aGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                              <  2:1328951/117‑1 (MQ=255)                 aGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                        <  1:1186519/139‑1 (MQ=255)                 aGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                        <  2:194493/139‑1 (MQ=255)                    gAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGc                                                                                                     <  1:381933/139‑1 (MQ=255)                                tCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                        <  2:1573319/124‑1 (MQ=255)                                tCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                        >  1:1573319/1‑124 (MQ=255)                                   ggCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGaa                                                                                                                    >  1:315365/1‑109 (MQ=255)                                   ggCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGaa                                                                                                                    <  2:315365/109‑1 (MQ=255)                                   ggCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGc                                                                                                     >  2:1659995/1‑124 (MQ=255)                                   ggCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGc                                                                                                     <  1:1659995/124‑1 (MQ=255)                                    gCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCg                                                                                     >  1:1602012/1‑139 (MQ=255)                                    gCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCg                                                                                     >  1:489612/1‑139 (MQ=255)                                     cTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGa                                                                                    >  2:119031/1‑139 (MQ=255)                                             gTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTg                                                                            <  2:1250075/139‑1 (MQ=255)                                                      aTGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGc                                                                   >  2:1069230/1‑139 (MQ=255)                                                          ttGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGtt                                                               >  2:758505/1‑139 (MQ=255)                                                           tGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGttt                                                              <  1:1568520/139‑1 (MQ=255)                                                                gctcgctGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACCGATcgttgat                                                                                                        >  2:734431/1‑86 (MQ=255)                                                                gctcgctGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACCGATcgttgat                                                                                                        <  1:734431/92‑7 (MQ=255)                                                                 ctcgctGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCacac                                                                                                                         >  1:1253267/1‑74 (MQ=255)                                                                 ctcgctGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCacac                                                                                                                         <  2:1253267/74‑1 (MQ=255)                                                                 ctcgctGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTa                                                                                           >  1:987788/1‑104 (MQ=255)                                                                 ctcgctGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTa                                                                                           <  2:987788/104‑1 (MQ=255)                                                                  tcgctGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGtt                                                       <  1:1069230/139‑1 (MQ=255)                                                                      tGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTcccc                                                   <  1:758505/139‑1 (MQ=255)                                                                             ggTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGAc                                            <  2:1602012/139‑1 (MQ=255)                                                                                    gCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGa                                     >  2:1297076/1‑139 (MQ=255)                                                                                        ccGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATggg                                 <  2:711346/139‑1 (MQ=255)                                                                                               gCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGa                          <  2:492356/139‑1 (MQ=255)                                                                                                catcatTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAAc                                                                                                                   <  1:1131813/49‑1 (MQ=255)                                                                                                catcatTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAAc                                                                                                                   >  2:1131813/1‑49 (MQ=255)                                                                                                       aCGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAAc                                                                                                                   <  2:988649/42‑1 (MQ=255)                                                                                                       aCGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAAc                                                                                                                   >  1:988649/1‑42 (MQ=255)                                                                                                        cGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGaa                                                                                                                    >  2:887956/1‑40 (MQ=255)                                                                                                        cGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGaa                                                                                                                    <  1:887956/40‑1 (MQ=255)                                                                                                             ttGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAgg                                                                              <  1:1233671/73‑1 (MQ=255)                                                                                                             ttGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAgg                                                                              >  2:1233671/1‑73 (MQ=255)                                                                                                             ttGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACcaa            >  2:508745/1‑139 (MQ=255)                                                                                                             ttGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACcaa            >  1:252076/1‑139 (MQ=255)                                                                                                               ggTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATggg                                 >  1:234937/1‑116 (MQ=255)                                                                                                               ggTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATggg                                 <  2:234937/116‑1 (MQ=255)                                                                                                               ggTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCgggg                            >  1:364664/1‑121 (MQ=255)                                                                                                               ggTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCgggg                            <  2:364664/121‑1 (MQ=255)                                                                                                               ggTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACcaaca          >  2:1734542/1‑139 (MQ=255)                                                                                                                 tgtTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAg        <  2:489612/139‑1 (MQ=255)                                                                                                                 tgtTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAg        <  1:508745/139‑1 (MQ=255)                                                                                                                  gtTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCttt                                                                                                  >  1:947334/1‑48 (MQ=255)                                                                                                                  gtTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCttt                                                                                                  <  2:947334/48‑1 (MQ=255)                                                                                                                  gtTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGc                                                                   <  1:1268010/79‑1 (MQ=255)                                                                                                                  gtTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGc                                                                   >  2:1268010/1‑79 (MQ=255)                                                                                                                   ttAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATggg                                 >  1:774732/1‑112 (MQ=255)                                                                                                                   ttAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATggg                                 <  2:774732/112‑1 (MQ=255)                                                                                                                       agagGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTcc  >  2:1191979/1‑139 (MQ=255)                                                                                                                           gTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAg        <  1:1423249/129‑1 (MQ=255)                                                                                                                           gTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAg        >  2:1423249/1‑129 (MQ=255)                                                                                                                              aTTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTcc                                                     >  1:376407/1‑81 (MQ=255)                                                                                                                              aTTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTcc                                                     <  2:376407/81‑1 (MQ=255)                                                                                                                                   |                                                                                                                               GGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCC  >  NC_000913/1781834‑1782090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑