Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 170,349 A→G H191R (CAC→CGC)  fhuC → iron(3+)‑hydroxamate import ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913170,3490AG100.0% 198.5 / NA 55H191R (CAC→CGC) fhuCiron(3+)‑hydroxamate import ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/28);  total (27/28)

CTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCACCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGATATCAATATGGCGGCACGCTACTGTGATTATCTGGTCGCCCTGCGCGGCGGTGAAATGATTGCTCAGGGAACGCCTGCGGAA  >  NC_000913/170223‑170479
                                                                                                                              |                                                                                                                                  
ctGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCa                                                                                                                        >  1:1294102/1‑139 (MQ=255)
  ggcggcGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAgg                                                                                                                      >  2:1695317/1‑139 (MQ=255)
      gcgAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGc                                                                                                                   >  1:791586/1‑138 (MQ=255)
      gcgAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGc                                                                                                                   <  2:791586/138‑1 (MQ=255)
                  gggCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGt                                                                                                      <  2:1138439/139‑1 (MQ=255)
                              ccATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGtt                                                                                                                               <  1:1623645/102‑1 (MQ=255)
                              ccATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGtt                                                                                                                               >  2:1623645/1‑102 (MQ=255)
                              ccATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGtt                                                                                          >  1:1528304/1‑139 (MQ=255)
                                aTGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGc                                                                                        <  2:1528304/139‑1 (MQ=255)
                                 tGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCa                                                                                       <  2:1294102/139‑1 (MQ=255)
                                 tGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCa                                                                                       <  1:1695317/139‑1 (MQ=255)
                                 tGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGAGATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCa                                                                                       <  2:134737/139‑1 (MQ=255)
                                  gCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCAc                                                                                      >  2:1084435/1‑139 (MQ=255)
                                     ggtggCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGat                                                                                   <  1:996640/139‑1 (MQ=255)
                                            cAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGATATCAata                                                                            >  1:1016419/1‑139 (MQ=255)
                                                aTAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGtt                                                                                                                               <  1:697212/84‑1 (MQ=255)
                                                aTAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGtt                                                                                                                               >  2:697212/1‑84 (MQ=255)
                                                aTAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGATATCAATATggc                                                                        >  2:1601628/1‑139 (MQ=255)
                                                  aGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGATATCAATATggcgg                                                                      <  2:803584/139‑1 (MQ=255)
                                                         gTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGc                                                                                                              <  1:1080794/92‑1 (MQ=255)
                                                         atCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGc                                                                                                              >  2:1080794/2‑92 (MQ=255)
                                                              ttGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCgtgt                                                                                           <  1:486375/106‑1 (MQ=255)
                                                              ttGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCgtgt                                                                                           >  2:486375/1‑106 (MQ=255)
                                                              ttGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGATATCAATATGGCGGCACGCTACtgtg                                                          >  1:679254/1‑139 (MQ=255)
                                                                 cTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGATATCAATATGGCGGCACGCTACTGTGAtt                                                       <  1:1084435/139‑1 (MQ=255)
                                                                               cTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGATATCAATATGGCGGCAc                                                                   <  2:1019192/113‑1 (MQ=255)
                                                                               cTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGATATCAATATGGCGGCAc                                                                   >  1:1019192/1‑113 (MQ=255)
                                                                                       tGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCa                                                                                                                        >  1:1402030/1‑52 (MQ=255)
                                                                                       tGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCa                                                                                                                        <  2:1402030/52‑1 (MQ=255)
                                                                                       tGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGa                                                                                                          <  1:1633063/66‑1 (MQ=255)
                                                                                       tGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGa                                                                                                          >  2:1633063/1‑66 (MQ=255)
                                                                                              cGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCa                                                                                       >  2:610971/1‑78 (MQ=255)
                                                                                              cGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCa                                                                                       <  1:610971/78‑1 (MQ=255)
                                                                                              cGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGATATCAATATGGCGGCACGCTACTGTGATTATCTGGTCGCCCTGcgcg                                     >  2:414223/1‑128 (MQ=255)
                                                                                              cGCCCACCAGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGATATCAATATGGCGGCACGCTACTGTGATTATCTGGTCGCCCTGcgcg                                     <  1:414223/128‑1 (MQ=255)
                                                                                                   accaGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGATATCAATATGGCGGCACGCTACTGTGATTATCTGGTCGCCCTGCGCGGCGGTGAAATg                          >  1:1185055/1‑134 (MQ=255)
                                                                                                   accaGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGATATCAATATGGCGGCACGCTACTGTGATTATCTGGTCGCCCTGCGCGGCGGTGAAATg                          <  2:1185055/134‑1 (MQ=255)
                                                                                                    ccaGGTTGATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGATATCAATATGGCGGCACGCTACTGTGATTATCTGGTCGCCCTGCGCGGCGGTGAAATGATTGCt                    <  2:679254/139‑1 (MQ=255)
                                                                                                           gATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCa                                                                                       >  2:1097026/1‑65 (MQ=255)
                                                                                                           gATGTGCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCa                                                                                       <  1:1097026/65‑1 (MQ=255)
                                                                                                              gtgCTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGATATCAATATGGCGGCa                                                                    >  2:282201/1‑81 (MQ=255)
                                                                                                              gtgCTGTCGCTGGTGCGACGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGATATCAATATGGCGGCa                                                                    <  1:282201/81‑1 (MQ=255)
                                                                                                                 cTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTg                                                                                                 <  1:772737/49‑1 (MQ=255)
                                                                                                                 cTGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTg                                                                                                 >  2:772737/1‑49 (MQ=255)
                                                                                                                  tGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCa                                                                                       <  2:957683/58‑1 (MQ=255)
                                                                                                                  tGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCa                                                                                       >  1:957683/1‑58 (MQ=255)
                                                                                                                  tGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGATATCAATATGGCGGCACGCTACTGTGATTATCTGGTCGCCCTGCGCGGCGGTGAAATGa                         >  1:961785/1‑120 (MQ=255)
                                                                                                                  tGTCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGATATCAATATGGCGGCACGCTACTGTGATTATCTGGTCGCCCTGCGCGGCGGTGAAATGa                         <  2:961785/120‑1 (MQ=255)
                                                                                                                    tCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGAc                                                                                                         <  1:916521/38‑1 (MQ=255)
                                                                                                                    tCGCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGAc                                                                                                         >  2:916521/1‑38 (MQ=255)
                                                                                                                      gCTGGTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGATATCAATATGGCGGCACGCTACTGTGATTATCTGGTCGCCCTGCGCGGCGGTGAAATGATTGCTCAGGGAACGCCTGCGGaa  >  1:1459478/1‑139 (MQ=255)
                                                                                                                         ggTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGt                                                                                                      <  1:956511/36‑1 (MQ=255)
                                                                                                                         ggTGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGt                                                                                                      >  2:956511/1‑36 (MQ=255)
                                                                                                                           tGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGATATCAATATGGCGGCACGCTACTGTGATTATCTGGTCGCCCTgcg                                       >  1:803587/1‑97 (MQ=255)
                                                                                                                           tGCGCCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGATATCAATATGGCGGCACGCTACTGTGATTATCTGGTCGCCCTgcg                                       <  2:803587/97‑1 (MQ=255)
                                                                                                                              |                                                                                                                                  
CTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCACCAGGTTGATGTGCTGTCGCTGGTGCACCGTTTAAGTCAGGAGCGTGGCCTGACGGTCATTGCCGTGTTGCACGATATCAATATGGCGGCACGCTACTGTGATTATCTGGTCGCCCTGCGCGGCGGTGAAATGATTGCTCAGGGAACGCCTGCGGAA  >  NC_000913/170223‑170479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑