Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,863,004 T→C R78R (CGT→CGC)  gapA → glyceraldehyde‑3‑phosphate dehydrogenase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,863,0040TC100.0% 336.4 / NA 92R78R (CGT→CGC) gapAglyceraldehyde‑3‑phosphate dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/46);  total (46/46)

GACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGT  >  NC_000913/1862870‑1863137
                                                                                                                                      |                                                                                                                                     
gACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATc                                                                                                                                   <  1:1545425/139‑1 (MQ=255)
   cTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCgg                                                                                                                                <  2:1374830/139‑1 (MQ=255)
   cTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCgg                                                                                                                                <  2:1660102/139‑1 (MQ=255)
   cTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCgg                                                                                                                                <  2:200963/139‑1 (MQ=255)
    tGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGc                                                                                                                               <  1:688190/139‑1 (MQ=255)
                        gCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAg                                                                                                           >  2:515707/1‑139 (MQ=255)
                               tGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGgtgt                                                                                                    >  2:1387601/1‑139 (MQ=255)
                                gCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGtt                                                                                                   <  1:515707/139‑1 (MQ=255)
                                  tGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCgg                                                                                                                                >  1:471613/1‑108 (MQ=255)
                                  tGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCgg                                                                                                                                <  2:471613/108‑1 (MQ=255)
                                  tGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGtt                                                                                                   <  1:322138/137‑1 (MQ=255)
                                  tGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGtt                                                                                                   >  2:322138/1‑137 (MQ=255)
                                  tGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGa                                                                                                 >  2:817241/1‑139 (MQ=255)
                                   gAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTaa                                                                                                                            <  1:1021667/111‑1 (MQ=255)
                                   gAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTaa                                                                                                                            >  2:1021667/1‑111 (MQ=255)
                                   gAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGAc                                                                                                >  2:210930/1‑139 (MQ=255)
                                     aaTATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGt                                                                                              <  2:211051/139‑1 (MQ=255)
                                          gACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTg                                                                                                        >  2:1328397/1‑124 (MQ=255)
                                          gACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTg                                                                                                        <  1:1328397/124‑1 (MQ=255)
                                            cTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAAc                                                                                                                           >  2:1152865/1‑103 (MQ=255)
                                            cTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAAc                                                                                                                           <  1:887383/103‑1 (MQ=255)
                                            cTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAAc                                                                                                                           >  2:887383/1‑103 (MQ=255)
                                            cTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAAc                                                                                                                           <  1:1152865/103‑1 (MQ=255)
                                              ccACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGa                                                                                     <  1:1267924/139‑1 (MQ=255)
                                              ccACTCACGGCCGTTTCGACGGTACCGTTGAAGTCAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGa                                                                                     <  1:1112647/139‑1 (MQ=255)
                                               cACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGaa                                                                                    >  2:1030925/1‑139 (MQ=255)
                                                aCTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAg                                                                                   >  2:1451701/1‑139 (MQ=255)
                                                 cTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGc                                                                                  >  1:675270/1‑139 (MQ=255)
                                                 cTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGc                                                                                  >  1:422494/1‑139 (MQ=255)
                                                 cTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGc                                                                                  >  1:1295831/1‑139 (MQ=255)
                                                        ccGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           <  1:210930/139‑1 (MQ=255)
                                                        ccGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           <  2:675270/139‑1 (MQ=255)
                                                           tttCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCt                                                                                       >  1:474672/1‑124 (MQ=255)
                                                           tttCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCt                                                                                       <  2:474672/124‑1 (MQ=255)
                                                           tttCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTg                                                                        >  2:1513926/1‑139 (MQ=255)
                                                            ttCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGaaa                                                                                                                     >  2:1096479/1‑93 (MQ=255)
                                                            ttCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGaaa                                                                                                                     <  1:1096479/93‑1 (MQ=255)
                                                            ttCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGt                                                                                              >  1:475229/1‑116 (MQ=255)
                                                            ttCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGt                                                                                              <  2:475229/116‑1 (MQ=255)
                                                              cGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTc                                                                     <  1:341080/139‑1 (MQ=255)
                                                                              gTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGt                                                                                                          >  1:687804/1‑86 (MQ=255)
                                                                              gTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGt                                                                                                          <  2:687804/86‑1 (MQ=255)
                                                                               tGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGc                                                    <  1:1529420/139‑1 (MQ=255)
                                                                                 aaaGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGAAGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGCCGTTGTCGCTGAACCAACTGGTCTGTGCctgtctcttatacacatct                                                  >  2:167542/1‑126 (MQ=255)
                                                                                   aGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGt                                                >  1:1592487/1‑139 (MQ=255)
                                                                                      cGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAg                                                                                                           <  1:1176838/77‑1 (MQ=255)
                                                                                      cGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAg                                                                                                           >  2:1176838/1‑77 (MQ=255)
                                                                                         tCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCaa                                                                                <  1:448067/101‑1 (MQ=255)
                                                                                         tCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCaa                                                                                >  2:448067/1‑101 (MQ=255)
                                                                                         tCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGt                                                >  2:685593/1‑133 (MQ=255)
                                                                                         tCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGt                                                <  1:685593/133‑1 (MQ=255)
                                                                                         tCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAacac                                          >  1:89583/1‑139 (MQ=255)
                                                                                           aTCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGt                                                >  1:880185/1‑131 (MQ=255)
                                                                                           aTCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGt                                                <  2:880185/131‑1 (MQ=255)
                                                                                           aTCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACAt                                        >  1:835227/1‑139 (MQ=255)
                                                                                                aTCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCg                                   >  1:1448912/1‑139 (MQ=255)
                                                                                                 tCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGc                                  <  1:1451701/139‑1 (MQ=255)
                                                                                                  cGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCt                                 >  1:413105/1‑139 (MQ=255)
                                                                                                   gTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTg                                >  2:38110/1‑139 (MQ=255)
                                                                                                      aaCGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGaaa                                                        <  1:1364331/112‑1 (MQ=255)
                                                                                                      aaCGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGaaa                                                        >  2:1364331/1‑112 (MQ=255)
                                                                                                       aCGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGaaa                                                                                                                     >  2:676287/1‑50 (MQ=255)
                                                                                                       aCGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGaaa                                                                                                                     <  1:676287/50‑1 (MQ=255)
                                                                                                       aCGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTaaa                                             <  2:14877/122‑1 (MQ=255)
                                                                                                       aCGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTaaa                                             >  1:14877/1‑122 (MQ=255)
                                                                                                       aCGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGc                            >  2:1251580/1‑139 (MQ=255)
                                                                                                          gTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTaaa                                             <  1:1351916/119‑1 (MQ=255)
                                                                                                          gTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTaaa                                             >  2:1351916/1‑119 (MQ=255)
                                                                                                          gTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCgaa                         >  2:1696517/1‑139 (MQ=255)
                                                                                                           tAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           <  1:770182/88‑1 (MQ=255)
                                                                                                           tAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           >  2:770182/1‑88 (MQ=255)
                                                                                                             aaaaaaTCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTg                                                     >  1:962467/1‑108 (MQ=255)
                                                                                                             aaaaaaTCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTg                                                     <  2:962467/108‑1 (MQ=255)
                                                                                                              aaaaaTCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGt                                                                                                          <  2:1620267/54‑1 (MQ=255)
                                                                                                              aaaaaTCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGt                                                                                                          >  1:1620267/1‑54 (MQ=255)
                                                                                                              aaaaaTCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGaaa                     <  2:89583/139‑1 (MQ=255)
                                                                                                              aaaaaTCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGaaa                     <  2:413105/139‑1 (MQ=255)
                                                                                                              aaaaaTCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGaaa                     <  1:1251580/139‑1 (MQ=255)
                                                                                                                 aaTCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTg                  <  1:435152/139‑1 (MQ=255)
                                                                                                                   tCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGt                <  1:1030925/139‑1 (MQ=255)
                                                                                                                   tCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGt                <  1:1513926/139‑1 (MQ=255)
                                                                                                                   tCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGt                <  2:1295831/139‑1 (MQ=255)
                                                                                                                               cTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTcc    >  2:296535/1‑139 (MQ=255)
                                                                                                                                 gAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAAc                                                       >  2:1249362/1‑86 (MQ=255)
                                                                                                                                 gAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAAc                                                       <  1:1249362/86‑1 (MQ=255)
                                                                                                                                 gAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAac                                            >  1:1585296/1‑97 (MQ=255)
                                                                                                                                 gAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAac                                            <  2:1585296/97‑1 (MQ=255)
                                                                                                                                 gAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGt  >  1:199968/1‑139 (MQ=255)
                                                                                                                                  aaCGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTc                                                                          >  1:1611026/1‑66 (MQ=255)
                                                                                                                                  aaCGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTc                                                                          <  2:1611026/66‑1 (MQ=255)
                                                                                                                                  aaCGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGt                <  2:1343287/124‑1 (MQ=255)
                                                                                                                                  aaCGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGt                >  1:1343287/1‑124 (MQ=255)
                                                                                                                                      |                                                                                                                                     
GACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGT  >  NC_000913/1862870‑1863137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑