Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,926,096	G→A	*219* (TAG→TAA)	yobB →	C‑N hydrolase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,926,096	0	G	A	100.0%	244.0 / NA	75	*219* (TAG→TAA)	yobB	C‑N hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/35);  total (40/35)

CAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGT  >  NC_000913/1925963‑1926229                                                                                                                                      |                                                                                                                                       cAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCttt                                                                                                                                   <  2:1665679/139‑1 (MQ=255)   aaTGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCttttt                                                                                                                                 <  2:654793/139‑1 (MQ=255)            ggTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGa                                                                                                                        >  2:73293/1‑139 (MQ=255)                 cAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATg                                                                                                                   <  2:772336/139‑1 (MQ=255)                 cAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATg                                                                                                                   <  2:475525/139‑1 (MQ=255)                        cTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGtt                                                                                                            <  2:258797/139‑1 (MQ=255)                        cTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGtt                                                                                                            >  2:1513198/1‑139 (MQ=255)                                 gAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGTGCATTAt                                                                                                   <  1:73293/139‑1 (MQ=255)                                   atatGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTg                                                                                                                          >  2:575461/1‑114 (MQ=255)                                   atatGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTg                                                                                                                          <  1:575461/114‑1 (MQ=255)                                        gtcgtcTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCtttt                                                                                                                                  <  2:1377570/101‑1 (MQ=255)                                        gtcgtcTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCtttt                                                                                                                                  >  1:1377570/1‑101 (MQ=255)                                            tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATTATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCTTGTTGCGcatcat                                                                                                   <  2:1003073/128‑1 (MQ=255)                                            tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGa                                                                                                                        <  2:479313/107‑1 (MQ=255)                                            tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGa                                                                                                                        >  1:479313/1‑107 (MQ=255)                                            tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCTTGTTGCGCATTAt                                                                                                   >  1:1003073/1‑128 (MQ=255)                                            tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGaaa                                                                                        >  1:1649244/1‑139 (MQ=255)                                            tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGaaa                                                                                        >  1:867410/1‑139 (MQ=255)                                            tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGaaa                                                                                        >  2:1107902/1‑139 (MQ=255)                                            tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGaaa                                                                                        >  2:1273866/1‑139 (MQ=255)                                                 tCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGc                                                                                   <  1:1107902/139‑1 (MQ=255)                                                  cGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCg                                                                                  >  1:861715/1‑139 (MQ=255)                                                     tCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGtt                                                                               <  2:1580412/139‑1 (MQ=255)                                                      cgcgCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                   >  1:286333/1‑118 (MQ=255)                                                      cgcgCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                   <  2:286333/118‑1 (MQ=255)                                                                       attattGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCa                                                                           >  1:183478/1‑125 (MQ=255)                                                                       attattGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCa                                                                           <  2:183478/125‑1 (MQ=255)                                                                       attattGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTg                                                            >  1:844943/1‑139 (MQ=255)                                                                        ttattGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTga                                                           >  2:1416355/1‑139 (MQ=255)                                                                              ttAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTg                                                                             <  1:557954/116‑1 (MQ=255)                                                                              ttAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTg                                                                             >  2:557954/1‑116 (MQ=255)                                                                                aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGc                                                                                                                             >  2:1107700/1‑66 (MQ=255)                                                                                aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGc                                                                                                                             <  1:1107700/66‑1 (MQ=255)                                                                                aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACa                                                                                            <  1:53307/99‑1 (MQ=255)                                                                                aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACa                                                                                            >  2:53307/1‑99 (MQ=255)                                                                                aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAAc                                                                                       <  1:1513979/104‑1 (MQ=255)                                                                                aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAAc                                                                                       >  2:1513979/1‑104 (MQ=255)                                                                                aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGAt                                                        >  1:840149/1‑134 (MQ=255)                                                                                aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGAt                                                        <  2:840149/134‑1 (MQ=255)                                                                                aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCct                                                   >  1:646208/1‑139 (MQ=255)                                                                                aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCct                                                   <  2:1550468/139‑1 (MQ=255)                                                                                aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCct                                                   <  1:1513198/139‑1 (MQ=255)                                                                                   cGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCCTCTg                                                >  1:1034745/1‑139 (MQ=255)                                                                                     gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACa                                                                                            >  2:416548/1‑94 (MQ=255)                                                                                     gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACa                                                                                            <  1:416548/94‑1 (MQ=255)                                                                                     gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCCTCTGtt                                              >  2:1507432/1‑139 (MQ=255)                                                                                     gTCAGCGTTCATCACAGGGTTGGCAAGGCGATAACATTCCATTACACTAAGCTTTTTCGGCCTGGCGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCC‑GGGAGGGATCGTTGAGATTGCCTCTGtt                                              >  2:189290/1‑139 (MQ=255)                                                                                      tCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                   >  2:1533964/1‑86 (MQ=255)                                                                                      tCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                   <  1:778910/86‑1 (MQ=255)                                                                                      tCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                   >  2:778910/1‑86 (MQ=255)                                                                                      tCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                   <  1:1533964/86‑1 (MQ=255)                                                                                      tCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCCTCTGTTg                                             >  2:1050364/1‑139 (MQ=255)                                                                                        aGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCa‑gggagg                                                                    >  2:148335/1‑114 (MQ=255)                                                                                        aGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCa‑gggagg                                                                    <  1:148335/114‑1 (MQ=255)                                                                                               atcaCAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCa‑gggaggg                                                                   <  2:120426/108‑1 (MQ=255)                                                                                               atcaCAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCa‑gggaggg                                                                   >  1:120426/1‑108 (MQ=255)                                                                                               atcaCAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCAtt                                     >  1:1616351/1‑139 (MQ=255)                                                                                                        ttGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGaaaaa                           >  1:677456/1‑139 (MQ=255)                                                                                                         tGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAAt                          >  2:862534/1‑139 (MQ=255)                                                                                                              aGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCa                     <  1:862534/139‑1 (MQ=255)                                                                                                                gCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATg                                                                                                                   >  2:849591/1‑44 (MQ=255)                                                                                                                gCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATg                                                                                                                   <  1:849591/44‑1 (MQ=255)                                                                                                                  gATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAccc                 >  1:1314460/1‑139 (MQ=255)                                                                                                                  gATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAccc                 >  2:737306/1‑139 (MQ=255)                                                                                                                   atatCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAcccc                <  2:861715/139‑1 (MQ=255)                                                                                                                       cATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGa            <  1:1507432/139‑1 (MQ=255)                                                                                                                          tCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTgcgc                                                                                                        <  1:413048/45‑1 (MQ=255)                                                                                                                          tCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTgcgc                                                                                                        >  2:413048/1‑45 (MQ=255)                                                                                                                           ccATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCCTCTGt                                               >  1:1500703/1‑100 (MQ=255)                                                                                                                           ccATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCCTCTGt                                               <  2:1500703/100‑1 (MQ=255)                                                                                                                            cATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCAc       <  1:1416355/139‑1 (MQ=255)                                                                                                                              ttACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                   >  2:649541/1‑46 (MQ=255)                                                                                                                              ttACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                   <  1:649541/46‑1 (MQ=255)                                                                                                                                aCGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTgg   <  2:677456/139‑1 (MQ=255)                                                                                                                                 cGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGt  >  2:232273/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                       CAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGT  >  NC_000913/1925963‑1926229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑