Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,022,606	G→A	*246* (TAG→TAA)	fliP →	flagellar biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,022,606	0	G	A	100.0%	219.3 / NA	67	*246* (TAG→TAA)	fliP	flagellar biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/33);  total (34/33)

CGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACG  >  NC_000913/2022474‑2022741                                                                                                                                     |                                                                                                                                        cGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGc                                                                                                                                   >  1:1644480/1‑139 (MQ=255) cGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGc                                                                                                                                   >  2:1306793/1‑139 (MQ=255) cGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTCGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTaaagagcc                                                                                                                                   >  2:1026446/1‑137 (MQ=255)      tGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAAt                                                                                                                              <  2:1644480/139‑1 (MQ=255)                 gggATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAAt                                                                                                                   <  2:929910/139‑1 (MQ=255)                 gggATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAAt                                                                                                                   <  2:487292/139‑1 (MQ=255)                       atgatgGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCa                                                                                                             <  2:1544671/139‑1 (MQ=255)                           tgGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGATGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGCGGCAAAATGACACCTGAATCGGTCatgat                                                                                                         >  2:1118581/1‑139 (MQ=255)                             gTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCatgatga                                                                                                       >  1:875236/1‑139 (MQ=255)                                 ccccAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAAt                                                                                                                              <  2:668350/112‑1 (MQ=255)                                 ccccAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAAt                                                                                                                              >  1:668350/1‑112 (MQ=255)                                 ccccAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGt                                                                                                               <  2:481067/127‑1 (MQ=255)                                 ccccAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGt                                                                                                               >  1:481067/1‑127 (MQ=255)                                 ccccAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATggg                                                                                                   >  1:928349/1‑139 (MQ=255)                                  cccAGCCACCATTGCTCTGCCCTTTAAACTGATGCTG‑TTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGa                                                                                                 >  2:523867/1‑139 (MQ=255)                                    cAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGAc                                                                                                >  2:1496848/1‑139 (MQ=255)                                        caccaTTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGaa                                                                                            <  2:1489039/139‑1 (MQ=255)                                        caccaTTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGaa                                                                                            >  2:1025991/1‑139 (MQ=255)                                             ttGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGc                                                                                                                                   >  1:203356/1‑95 (MQ=255)                                             ttGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGc                                                                                                                                   <  2:203356/95‑1 (MQ=255)                                                     cccTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACGGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTcg                                                                               >  1:320875/1‑139 (MQ=255)                                                     cccTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGC‑AAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTcgc                                                                              >  2:1184399/1‑139 (MQ=255)                                                      ccTTTAAACTGATGCTG‑TTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTcgcg                                                                             <  1:523867/139‑1 (MQ=255)                                                                 aTGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAG‑TAAAGAGGCAAAATGACACCTGAATCGGTCatg                                                                                                           >  1:1679697/1‑98 (MQ=255)                                                                 aTGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAG‑TAAAGAGGCAAAATGACACCTGAATCGGTCatg                                                                                                           <  2:1679697/98‑1 (MQ=255)                                                                  tGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGc                                                                  <  1:1306793/139‑1 (MQ=255)                                                                  tGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGc                                                                  <  1:1026446/139‑1 (MQ=255)                                                                     tGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGAAGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGc                                                               <  1:778119/139‑1 (MQ=255)                                                                       tttGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGt                                                                                                               >  1:962222/1‑89 (MQ=255)                                                                       tttGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGt                                                                                                               <  2:962222/89‑1 (MQ=255)                                                                         tGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCg                                                           <  1:1025991/139‑1 (MQ=255)                                                                           tACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCt                                                         >  1:1505810/1‑139 (MQ=255)                                                                               ggtggATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGc                                                                        <  1:101581/120‑1 (MQ=255)                                                                               ggtggATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGc                                                                        >  2:101581/1‑120 (MQ=255)                                                                                gtggATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGacac                                                                                                                         >  1:716322/1‑70 (MQ=255)                                                                                gtggATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGacac                                                                                                                         <  2:716322/70‑1 (MQ=255)                                                                                 tggATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTAttgtt                                                   >  1:444585/1‑139 (MQ=255)                                                                                   gATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAAt                                                                                                                   >  1:772265/1‑73 (MQ=255)                                                                                   gATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAAt                                                                                                                   <  2:772265/73‑1 (MQ=255)                                                                                        ctggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCg                                                           >  2:42239/1‑124 (MQ=255)                                                                                        ctggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCg                                                           <  1:42239/124‑1 (MQ=255)                                                                                         tggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGt                                           <  2:928349/139‑1 (MQ=255)                                                                                          ggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAAt                                                                                                                              >  1:956225/1‑55 (MQ=255)                                                                                          ggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAAt                                                                                                                              <  2:956225/55‑1 (MQ=255)                                                                                          ggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGaa                                                                                            >  1:649873/1‑89 (MQ=255)                                                                                          ggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGaa                                                                                            <  2:649873/89‑1 (MQ=255)                                                                                          ggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGTTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGtt                                          >  2:568808/1‑139 (MQ=255)                                                                                          ggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGcc                                                              <  2:1183622/119‑1 (MQ=255)                                                                                          ggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGcc                                                              >  1:1183622/1‑119 (MQ=255)                                                                                             aaTTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGt                                       >  1:266767/1‑139 (MQ=255)                                                                                               ttGCTGGTCGGTTCGCTGGCGCAGATCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCa                                     <  1:1118581/139‑1 (MQ=255)                                                                                               ttGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCa                                     <  1:1496848/139‑1 (MQ=255)                                                                                               ttGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCa                                     <  1:95066/139‑1 (MQ=255)                                                                                               ttGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCa                                     <  2:227692/139‑1 (MQ=255)                                                                                               ttGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGC‑AAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCAc                                    <  1:1184399/139‑1 (MQ=255)                                                                                                tGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCAc                                    <  2:444585/139‑1 (MQ=255)                                                                                                  cTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAAt                                                                                                                   <  2:571577/58‑1 (MQ=255)                                                                                                  cTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAAt                                                                                                                   >  1:571577/1‑58 (MQ=255)                                                                                                     gTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCatga                                                                                                          >  1:1419399/1‑64 (MQ=255)                                                                                                     gTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCatga                                                                                                          <  2:1419399/64‑1 (MQ=255)                                                                                                      tCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCt                              >  1:1236700/1‑139 (MQ=255)                                                                                                       cGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTcgcg                                                                             <  2:851191/91‑1 (MQ=255)                                                                                                       cGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTcgcg                                                                             >  1:851191/1‑91 (MQ=255)                                                                                                          ttCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTatc                          >  1:32168/1‑139 (MQ=255)                                                                                                            cGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTatcat                        >  2:1404085/1‑139 (MQ=255)                                                                                                                       agCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGc             >  2:170153/1‑139 (MQ=255)                                                                                                                           tttACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGgc         >  2:1346149/1‑139 (MQ=255)                                                                                                                                  cTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACg  <  2:333835/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        CGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACG  >  NC_000913/2022474‑2022741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑