| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,022,810 | C→T | A65A (GCC→GCT) | fliQ → | flagellar biosynthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,022,810 | 0 | C | T | 97.9% | 149.1 / ‑4.1 | 48 | A65A (GCC→GCT) | fliQ | flagellar biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (24/23); total (25/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
GGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATATCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCT > NC_000913/2022702‑2022944 | ggTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGAt < 1:266228/139‑1 (MQ=255) tCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTa > 2:378335/1‑139 (MQ=255) gggCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTc > 2:1631628/1‑139 (MQ=255) ccTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGc < 2:1505810/139‑1 (MQ=255) ttATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCAc > 1:248602/1‑139 (MQ=255) tATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCAcc < 2:303871/139‑1 (MQ=255) aGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCa > 2:1106157/1‑139 (MQ=255) tGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGa < 1:1690416/93‑1 (MQ=255) tGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGa > 2:1690416/1‑93 (MQ=255) gCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTg < 2:248602/139‑1 (MQ=255) cAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGa < 1:310624/91‑1 (MQ=255) cAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGa > 2:310624/1‑91 (MQ=255) gccgccACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGa > 1:71602/1‑88 (MQ=255) gccgccACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGa < 2:71602/88‑1 (MQ=255) gccgccACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTTCTGGATTACGTCCGCa < 1:1077557/118‑1 (MQ=255) gccgccACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGc < 1:288477/103‑1 (MQ=255) gccgccACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGc > 2:288477/1‑103 (MQ=255) gccgccACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCa > 2:1077557/1‑118 (MQ=255) cgccACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGtt < 2:292613/99‑1 (MQ=255) cgccACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGtt > 1:292613/1‑99 (MQ=255) ccACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTatat > 2:687143/1‑139 (MQ=255) gATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATATCATCgga < 1:378335/139‑2 (MQ=255) aTTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAAc < 2:761968/120‑1 (MQ=255) aTTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAAc > 1:761968/1‑120 (MQ=255) cGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATATCATCGGGTAACCg < 1:57533/139‑1 (MQ=255) tGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGAt < 2:1321405/86‑1 (MQ=255) tGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGAt > 1:1321405/1‑86 (MQ=255) aCGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATATCATCGGGTAACCGTACTATg > 2:1136044/1‑139 (MQ=255) cGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGc > 2:855334/1‑77 (MQ=255) cGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGc < 1:855334/77‑1 (MQ=255) cGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATATCATCGGGTaa > 2:440577/1‑112 (MQ=255) cGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATATCATCGGGTaa < 1:440577/113‑2 (MQ=255) cGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATATCATCGGGTAACCGTACTATGTTGCAGGTGACAAGCg > 1:660769/1‑139 (MQ=255) cGAAAATCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATATCATCGGGTAACCGTACTATGTTGCAGGTGACAAGCg > 1:657495/1‑139 (MQ=255) aaaaTCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTgg > 1:1236071/1‑45 (MQ=255) aaaaTCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTgg < 2:1236071/45‑1 (MQ=255) aaaaTCATCGCCGTATTTATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATATCATCGGGTAACCGTACTATGTTGCAGGTGACAAGCGaa > 1:1335836/1‑139 (MQ=255) ttATCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATATCATCGGGTAACCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGt < 1:1631628/139‑1 (MQ=255) tCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATATCATCGGGTAACCGTACTATGTTGCAGGTGACAAGCGAAc < 1:714823/121‑1 (MQ=255) tCGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATATCATCGGGTAACCGTACTATGTTGCAGGTGACAAGCGAAc > 2:714823/1‑121 (MQ=255) cGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTatat < 2:829068/81‑1 (MQ=255) cGCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTatat > 1:829068/1‑81 (MQ=255) gCCATTATTATTGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATATCATCGGGTAACCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAAc > 1:1703253/1‑139 (MQ=255) tattattGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATATCATCGGGTAACCGTACTATGTTg > 1:442754/1‑98 (MQ=255) tattattGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATATCATCGGGTAACCGTACTATGTTg < 2:442754/98‑1 (MQ=255) ttGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATATCATCGGGTAACCGTACTATGTTGCAGGTGACAAg > 1:457331/1‑104 (MQ=255) ttGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATATCATCGGGTAACCGTACTATGTTGCAGGTGACAAg < 2:457331/104‑1 (MQ=255) ttGCTGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATATCATCGGGTAACCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCt < 1:687143/139‑1 (MQ=255) | GGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATATCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCT > NC_000913/2022702‑2022944 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |