| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,051,158 | A→G | D2074G (GAC→GGC) | yeeJ → | putative adhesin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,051,158 | 0 | A | G | 88.7% | 170.5 / 9.0 | 62 | D2074G (GAC→GGC) | yeeJ | putative adhesin |
| Reads supporting (aligned to +/- strand): ref base A (4/3); new base G (26/29); total (30/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01 | |||||||||||
GAGCGATGACAGGTAGCGTCACGGTAAGCGCAGTCACGACCGCTGGTGGAATGCAAACAGTAGATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGACTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAATTTGTGCAATCAGGTACTAACGTGCCCTATATAAAAATTAGCGCAATTGATTACA > NC_000913/2051020‑2051292 | gAGCGATGACAGGTAGCGTCACGGTAAGCGCAGTCACGACCGCTGGTGGAATGCAAACAGTAGATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGa < 1:628806/139‑1 (MQ=255) gATGACAGGTAGCGTCACGGTAAGCGCAGTCACGACCGCTGGTGGAATGCAAACAGTAGATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCtat < 1:713860/139‑1 (MQ=255) cAGGTAGCGTCACGGTAAGCGCAGTCACGACCGCTGGTGGAATGCAAACAGTAGATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGa < 2:382356/139‑1 (MQ=255) cGTCACGGTAAGCGCAGTCACGACCGCTGGTGGAATGCAAACAGTAGATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCt > 1:722380/1‑139 (MQ=255) aCGGTAAGCGCAGTCACGACCGCTGGTGGAATGCAAACAGTAGATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCA‑‑‑‑GTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACg > 2:652890/1‑139 (MQ=255) ggTAAGCGCAGTCACGACCGCTGGTGGAATGCAAACAGTAGATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTa > 2:805094/1‑139 (MQ=255) gcgcAGTCACGACCGCTGGTGGAATGCAAACAGTAGATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAAtt > 2:422347/1‑133 (MQ=255) gcgcAGTCACGACCGCTGGTGGAATGCAAACAGTAGATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAAtt < 1:422347/133‑1 (MQ=255) cGACCGCTGGTGGAATGCAAACAGTAGATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCa < 1:805094/139‑1 (MQ=255) gACCGCTGGTGGAATGCAAACAGTAGATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCAc < 1:1166305/139‑1 (MQ=255) aCCGCTGGTGGAATGCAAACAGTAGATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGACTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACg > 1:499528/1‑139 (MQ=255) tggtggAATGCAAACAGTAGATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACg > 2:1270664/1‑134 (MQ=255) tggtggAATGCAAACAGTAGATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACg < 1:1270664/134‑1 (MQ=255) ggtggAATGCAAACAGTAGATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGatatat < 2:1371828/139‑1 (MQ=255) aaTGCAAACAGTAGATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGc < 2:1263962/139‑1 (MQ=255) aaTGCAAACAGTAGATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGACTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGc < 2:499528/139‑1 (MQ=255) cAAACAGTAGATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCCGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATAACGAGAGTGCTGAATTACGTCTTTTTCTGCACGATATATCAGGCAAta > 2:1107913/1‑138 (MQ=255) gTAGATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCa > 2:762262/1‑139 (MQ=255) gATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACg > 1:1070249/1‑115 (MQ=255) gATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACg < 2:1070249/115‑1 (MQ=255) gCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCtat > 1:177087/1‑73 (MQ=255) gCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCtat < 2:177087/73‑1 (MQ=255) ggtggCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATCGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAggg > 1:986017/1‑139 (MQ=255) gtggCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAAt < 1:1369777/117‑1 (MQ=255) gtggCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAAt > 2:1369777/1‑117 (MQ=255) gtggCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGa > 2:865576/1‑139 (MQ=255) gtggCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGACTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGa > 1:804464/1‑139 (MQ=255) gtggCTGGCCCGGCAGACACCTCCCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGATTTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGa > 1:920439/1‑139 (MQ=255) tggctggcCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGAt < 1:1107913/139‑1 (MQ=255) tggcCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTg > 1:1145357/1‑94 (MQ=255) tggcCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTg < 2:1145357/94‑1 (MQ=255) tggcCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGaa < 2:722380/139‑1 (MQ=255) ggcCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAAt < 2:539601/139‑1 (MQ=255) gcCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAAtt > 1:1166991/1‑139 (MQ=255) aGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGACTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAATTTGTGCaa < 2:804464/139‑1 (MQ=255) acacCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATCGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAATTTGTGCAATc < 2:986017/139‑1 (MQ=255) acacCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAATTTGTGCAATc < 1:865576/139‑1 (MQ=255) tggCAGTCCAGCCTTAAGAGGAATCGGGCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATGACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAATTTGTGCAATCAGGTa < 2:920439/137‑1 (MQ=255) cGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAATTTGTGCAATCAGGTAc > 2:394579/1‑139 (MQ=255) tccgtccTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGa < 2:481761/107‑1 (MQ=255) tccgtccTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGa > 1:481761/1‑107 (MQ=255) ccgtccTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGtt < 1:232732/68‑1 (MQ=255) ccgtccTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGtt > 2:232732/1‑68 (MQ=255) agtccTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAATTTGTGCAATCAGGTACTAACGTg < 1:652890/138‑1 (MQ=255) tccTTAAGAGCAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAATTTGTGCAATCAGGTACTAACGTGcc > 1:1336802/1‑139 (MQ=255) agCAATCGGTCAACACTGAAAGGGGACTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAATTTGTGCAATCAGGTACTAACGTGCCCTATATaa > 2:300040/1‑139 (MQ=255) cAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGaa < 1:861499/43‑1 (MQ=255) cAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGaa > 2:861499/1‑43 (MQ=255) cAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGAt < 2:1345532/81‑1 (MQ=255) cAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGAt > 1:1345532/1‑81 (MQ=255) cAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAg < 2:1394466/95‑1 (MQ=255) cAATCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAg > 1:1394466/1‑95 (MQ=255) tCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAATTTGTGCa > 2:1432356/1‑108 (MQ=255) tCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAATTTGTGCa < 1:1432356/108‑1 (MQ=255) tCGGTCATCACTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAATTTGTGCAATCAGGTACTAACGTGCCCTATATAAAAAtt > 1:1406029/1‑139 (MQ=255) catcaCTGAAAGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAATTTGTGCAATCAGGTACTAACGTGCCCTATATAAAAATTAgcgc > 1:794693/1‑139 (MQ=255) aaaGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAATTTGTGCAATCAGGTACTAAc < 2:1595015/108‑1 (MQ=255) aaaGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAATTTGTGCAATCAGGTACTAAc > 1:1595015/1‑108 (MQ=255) aaGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAATTTGTGCAATCAGGTACTAACGTGCCCTATATAAAAATTAGCGCAATTGATTa < 2:1336802/139‑1 (MQ=255) aaGGGGGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAATTTGTGCAATCAGGTACTAACGTGCCCTATATAAAAATTAGCGCAATTGATTa < 2:794693/139‑1 (MQ=255) ggggGCTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAATTTGTGCAATCAGGTACTAACGTGCCCTATATAAAAATTAGCGCAATTGATTACa < 1:394579/139‑1 (MQ=255) ggACTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAATTTGTGCAATc < 2:1106950/93‑1 (MQ=255) ggACTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAATTTGTGCAATc > 1:1106950/1‑93 (MQ=255) | GAGCGATGACAGGTAGCGTCACGGTAAGCGCAGTCACGACCGCTGGTGGAATGCAAACAGTAGATATAACGCTGGTGGCTGGCCCGGCAGACACCTCGCAGTCCGTCCTTAAGAGCAATCGGTCATCACTGAAAGGGGACTATACCGATAGTGCTGAATTACGTCTTGTTCTGCACGATATATCAGGCAATCCGATCAAAGTTTCTGAAGGGATGGAATTTGTGCAATCAGGTACTAACGTGCCCTATATAAAAATTAGCGCAATTGATTACA > NC_000913/2051020‑2051292 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |