Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,115,907	C→T	*407* (TAG→TAA)	wcaL ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,115,907	0	C	T	100.0%	98.8 / NA	31	*407* (TAG→TAA)	wcaL	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/15);  total (16/15)

AACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAACGCCGCCAGTCG  >  NC_000913/2115792‑2116041                                                                                                                    |                                                                                                                                       aaCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGc                                                                                                                 >  2:664848/1‑139 (MQ=255) aaCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGc                                                                                                                 <  1:401658/139‑1 (MQ=255)        tGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTtct                                                                                                          <  1:46967/139‑1 (MQ=255)          tCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGtt                                                                                                            <  2:495520/135‑1 (MQ=255)          tCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGtt                                                                                                            >  1:495520/1‑135 (MQ=255)              gcgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTg                                                                                                    <  2:743720/139‑1 (MQ=255)                      cgcgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctg                                                                                            <  1:1056174/139‑1 (MQ=255)                      cgcgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctg                                                                                            <  1:664848/139‑1 (MQ=255)                            ggCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctg                                                                                            >  2:469576/1‑133 (MQ=255)                            ggCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctg                                                                                            <  1:469576/133‑1 (MQ=255)                                       tataGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGc                                                                                                                                >  1:1176953/1‑86 (MQ=255)                                       tATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGc                                                                                                                                <  2:1176953/86‑1 (MQ=255)                                                 ggCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGAt                                                                                                      <  1:445639/102‑1 (MQ=255)                                                 ggCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGAt                                                                                                      >  2:445639/1‑102 (MQ=255)                                                     aGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGc                                                                                                                 <  1:545297/87‑1 (MQ=255)                                                     aGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGc                                                                                                                 >  2:545297/1‑87 (MQ=255)                                                     aGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgc                                                             >  1:267403/1‑139 (MQ=255)                                                      gcgcCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGcc                                                                                                                               >  2:930536/1‑72 (MQ=255)                                                      gcgcCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGcc                                                                                                                               <  1:930536/72‑1 (MQ=255)                                                            aGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGtt                                                      >  2:1510472/1‑139 (MQ=255)                                                               ttGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTga                                                   <  1:1510472/139‑1 (MQ=255)                                                                   cGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctg                                                                                            <  2:1631039/94‑1 (MQ=255)                                                                   cGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctg                                                                                            >  1:1631039/1‑94 (MQ=255)                                                                                cGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATtcg                                  >  2:805942/1‑139 (MQ=255)                                                                                                     ggCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGc                                                                                                                 <  2:1579379/39‑1 (MQ=255)                                                                                                     ggCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGc                                                                                                                 >  1:1579379/1‑39 (MQ=255)                                                                                                      gCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAAcg            >  1:534337/1‑139 (MQ=255)                                                                                                       cATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAAcgc           >  1:225312/1‑139 (MQ=255)                                                                                                       cATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAAcgc           >  2:699448/1‑139 (MQ=255)                                                                                                         tGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctgct                                                                                          <  1:888781/58‑1 (MQ=255)                                                                                                         tGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctgct                                                                                          >  2:888781/1‑58 (MQ=255)                                                                                                                tccttTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAACGCCGCCAGTCg  >  1:931987/1‑139 (MQ=255)                                                                                                                tccttTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAACGCCGCCAGTCg  <  2:267403/139‑1 (MQ=255)                                                                                                                tccttTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAACGCCGCCAGTCg  <  1:699448/139‑1 (MQ=255)                                                                                                                    |                                                                                                                                       AACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAACGCCGCCAGTCG  >  NC_000913/2115792‑2116041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑