Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,131,667	T→C	D135G (GAC→GGC)	wcaC ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,131,667	0	T	C	100.0%	111.0 / NA	31	D135G (GAC→GGC)	wcaC	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/14);  total (17/14)

TGTGCGCGATCAATCTTCACCGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGTCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCGCTGT  >  NC_000913/2131550‑2131797                                                                                                                      |                                                                                                                                   tgtgCGCGATCAATCTTCACCGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCagag                                                                                                               <  2:1211989/139‑1 (MQ=255)                        cggATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCa                                                                                                                              >  1:1574437/1‑101 (MQ=255)                        cggATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCa                                                                                                                              <  2:1574437/101‑1 (MQ=255)                          gATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCAc                                                                                      >  1:1533979/1‑139 (MQ=255)                                        gTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCa                                                                                                                              <  2:1336164/85‑1 (MQ=255)                                        gTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCa                                                                                                                              >  1:1336164/1‑85 (MQ=255)                                          cGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTccagaccaga                                                                                                                <  2:85679/97‑1 (MQ=255)                                          cGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTccagaccaga                                                                                                                >  1:85679/1‑97 (MQ=255)                                            ggCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTc                                                                                                                         >  2:1703159/1‑86 (MQ=255)                                            ggCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTc                                                                                                                         <  1:1703159/86‑1 (MQ=255)                                            ggCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCa                                                                             <  2:1407235/130‑1 (MQ=255)                                            ggCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCa                                                                             >  1:1407235/1‑130 (MQ=255)                                            ggCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcac                                                                    >  1:287585/1‑139 (MQ=255)                                            ggCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcac                                                                    >  2:1380518/1‑139 (MQ=255)                                            ggCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcac                                                                    >  1:1054447/1‑139 (MQ=255)                                            ggCATTTCTGGCAGCCTATTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcac                                                                    >  1:1084636/1‑139 (MQ=255)                                               aTTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcaccac                                                                 <  2:287585/139‑1 (MQ=255)                                                tttCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACg                                                                <  2:1084636/139‑1 (MQ=255)                                                      gCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTa                                                          >  2:1561049/1‑139 (MQ=255)                                                          ccTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcacca                                                                  >  1:43929/1‑127 (MQ=255)                                                          ccTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcacca                                                                  <  2:43929/127‑1 (MQ=255)                                                                ttCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGcc                                                <  2:1054447/139‑1 (MQ=255)                                                                        ttCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACg                                                                >  1:319087/1‑115 (MQ=255)                                                                        ttCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACg                                                                <  2:319087/115‑1 (MQ=255)                                                                          cGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAgcg                                                                       >  2:1682960/1‑106 (MQ=255)                                                                          cGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAgcg                                                                       <  1:1682960/106‑1 (MQ=255)                                                                                                gCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACg                                                                <  1:1708008/91‑1 (MQ=255)                                                                                                gCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACg                                                                >  2:1708008/1‑91 (MQ=255)                                                                                                     cGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCgg           <  1:1384692/139‑1 (MQ=255)                                                                                                           cacTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCGc     >  2:461499/1‑139 (MQ=255)                                                                                                              tCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCGCtgt  >  2:862999/1‑139 (MQ=255)                                                                                                                      |                                                                                                                                   TGTGCGCGATCAATCTTCACCGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGTCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCGCTGT  >  NC_000913/2131550‑2131797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑