Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,139,759	C→T	*618* (TAG→TAA)	asmA ←	suppressor of OmpF assembly mutants, putative outer membrane protein assembly factor, inner membrane‑anchored periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,139,759	0	C	T	100.0%	227.5 / NA	70	*618* (TAG→TAA)	asmA	suppressor of OmpF assembly mutants, putative outer membrane protein assembly factor, inner membrane‑anchored periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/33);  total (37/33)

AGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCACTTGCAGGC  >  NC_000913/2139627‑2139889                                                                                                                                     |                                                                                                                                   agtagCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACAtc                                                                                                                              >  2:50238/1‑139 (MQ=255)     gCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACAtcttct                                                                                                                          <  1:50238/139‑1 (MQ=255)          gAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGc                                                                                                                     >  1:388228/1‑139 (MQ=255)                   gTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATAATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACttcttc                                                                                                            <  2:1547993/139‑1 (MQ=255)                        cGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATc                                                                                                       >  1:1027598/1‑139 (MQ=255)                                  ttCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTca                                                                                                           >  2:1095838/1‑125 (MQ=255)                                  ttCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTca                                                                                                           <  1:1095838/125‑1 (MQ=255)                                          tACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATc                                                                                     >  1:314178/1‑139 (MQ=255)                                            cATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGGCTGGTCACCTGGTAACTTACATCTTCTCCAGCAAc                                                                                                                  <  1:1149448/108‑1 (MQ=255)                                            cATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGGCTGGTCACCTGGTAACTTACATCTTCTCCAGCAAc                                                                                                                  >  2:1149448/1‑108 (MQ=255)                                                 tCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGc                                                                                                                     <  2:1199682/100‑1 (MQ=255)                                                 tCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGc                                                                                                                     >  1:1199682/1‑100 (MQ=255)                                                 tCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAAc                                                                                                                  <  2:1172193/103‑1 (MQ=255)                                                 tCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTc                                                                              >  1:1270756/1‑139 (MQ=255)                                                 tCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTGATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAAc                                                                                                                  >  1:1172193/1‑103 (MQ=255)                                                   aGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCg                                                                            <  2:1027598/139‑1 (MQ=255)                                                     aaTATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAAc                                                                                                                  >  2:826090/1‑99 (MQ=255)                                                     aaTATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAAc                                                                                                                  >  1:85922/1‑99 (MQ=255)                                                     aaTATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAAc                                                                                                                  <  2:85922/99‑1 (MQ=255)                                                     aaTATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAAc                                                                                                                  <  1:826090/99‑1 (MQ=255)                                                     aaTATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCttt                                                                                                    <  2:745490/113‑1 (MQ=255)                                                     aaTATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCttt                                                                                                    >  1:745490/1‑113 (MQ=255)                                                     aaTATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCt                                                                          <  1:618057/139‑1 (MQ=255)                                                          aaTGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAAc                                                                                                                  <  1:1547583/94‑1 (MQ=255)                                                          aaTGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAAc                                                                                                                  >  2:1547583/1‑94 (MQ=255)                                                          aaTGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGc                                                                                                  >  2:1543337/1‑110 (MQ=255)                                                          aaTGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGc                                                                                                  <  1:1543337/110‑1 (MQ=255)                                                           atgatgCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTa                                                                                             <  1:619548/114‑1 (MQ=255)                                                           atgatgCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTa                                                                                             >  2:619548/1‑114 (MQ=255)                                                               tgCCGCCACGAACGGCTGATGCGACGCTTTACGCGTCTTACCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATTTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTc                                                                >  1:1055340/1‑139 (MQ=255)                                                                 ccgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCAt                                                              >  2:1027171/1‑139 (MQ=255)                                                                 ccgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCAt                                                              >  2:446600/1‑139 (MQ=255)                                                                   gccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCt                                                                          <  1:342924/125‑1 (MQ=255)                                                                   gccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCt                                                                          >  2:342924/1‑125 (MQ=255)                                                                    ccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCa                                                           >  2:1708590/1‑139 (MQ=255)                                                                        gAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCg                                                       <  1:1708590/139‑1 (MQ=255)                                                                                 aTGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                         <  2:214045/96‑1 (MQ=255)                                                                                 aTGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                         >  1:214045/1‑96 (MQ=255)                                                                                  tGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCt                                             <  1:446600/139‑1 (MQ=255)                                                                                  tGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCt                                             <  2:1270756/139‑1 (MQ=255)                                                                                      aCGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGt                                         >  1:1606639/1‑139 (MQ=255)                                                                                        gCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTcc                                       >  2:926337/1‑139 (MQ=255)                                                                                                   cTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCaa                                                                                                                   >  2:1316703/1‑52 (MQ=255)                                                                                                   cTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCaa                                                                                                                   <  1:1316703/52‑1 (MQ=255)                                                                                                      aTCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCttt                                                                                                    >  2:1371259/1‑64 (MQ=255)                                                                                                      aTCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCttt                                                                                                    <  1:1371259/64‑1 (MQ=255)                                                                                                       tCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTgcgc                        <  2:388228/139‑1 (MQ=255)                                                                                                           gCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTgcgc                        <  2:1158340/135‑1 (MQ=255)                                                                                                           gCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTa                    >  1:1117698/1‑139 (MQ=255)                                                                                                           gCCTACAGGACTGGTCACCTGGTAACTTAAATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCGTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTgcgc                        >  1:1158340/1‑135 (MQ=255)                                                                                                              tACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCt                                      >  1:818726/1‑118 (MQ=255)                                                                                                              tACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCt                                      <  2:818726/118‑1 (MQ=255)                                                                                                                cAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTca                                                                                                           <  2:1438132/47‑1 (MQ=255)                                                                                                                cAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTca                                                                                                           >  1:1438132/1‑47 (MQ=255)                                                                                                                cAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCttt                                                                                                    >  2:351182/1‑54 (MQ=255)                                                                                                                cAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCttt                                                                                                    <  1:351182/54‑1 (MQ=255)                                                                                                                     cTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                         <  1:1436439/60‑1 (MQ=255)                                                                                                                     cTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                         >  2:1436439/1‑60 (MQ=255)                                                                                                                     cTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCAc          >  2:781036/1‑139 (MQ=255)                                                                                                                        gTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGc                                                                      <  2:483901/76‑1 (MQ=255)                                                                                                                        gTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGc                                                                      >  1:483901/1‑76 (MQ=255)                                                                                                                        gTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCg                                                       >  2:4055/1‑91 (MQ=255)                                                                                                                        gTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCg                                                       <  1:4055/91‑1 (MQ=255)                                                                                                                        gTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCt                                      <  1:1513046/108‑1 (MQ=255)                                                                                                                        gTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCt                                      >  2:1513046/1‑108 (MQ=255)                                                                                                                        gTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTgcg                         <  1:263073/121‑1 (MQ=255)                                                                                                                        gTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTgcg                         >  2:263073/1‑121 (MQ=255)                                                                                                                        gTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCACTTg       >  2:391827/1‑139 (MQ=255)                                                                                                                            ccTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCACTTGCAgg   <  1:391827/139‑1 (MQ=255)                                                                                                                             cTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCACTTGCAGGc  <  1:926337/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                   AGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCACTTGCAGGC  >  NC_000913/2139627‑2139889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑