| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,152,876 | G→A | P85S (CCG→TCG) | yegL ← | VMA domain protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,152,876 | 0 | G | A | 98.3% | 189.0 / ‑4.2 | 60 | P85S (CCG→TCG) | yegL | VMA domain protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (32/27); total (32/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.81e-01 | |||||||||||
GTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑GAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGT > NC_000913/2152740‑2153007 | gTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGAATCGCCCTGGGCAAACAGGATGGGCG‑aaa > 1:1174142/1‑139 (MQ=255) ggAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAAtt > 1:1346555/1‑139 (MQ=255) aaTCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAgcg > 1:100601/1‑139 (MQ=255) tCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGC‑‑GAAAAAAATTAgcggcg < 2:555801/139‑1 (MQ=255) ccACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAgcggcg < 2:1346555/139‑1 (MQ=255) ccACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAgcggcg < 1:1382145/139‑1 (MQ=255) gggCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTgg > 1:174836/1‑139 (MQ=255) ataataCGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAg > 1:1022903/1‑139 (MQ=255) ataataCGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAg > 2:643476/1‑139 (MQ=255) ataataCGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAg > 1:1141281/1‑139 (MQ=255) gAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGgttgtt > 1:897215/1‑139 (MQ=255) tACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCcac > 2:1199080/1‑139 (MQ=255) tACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCcac < 2:100601/139‑1 (MQ=255) tttGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCa < 2:648432/139‑1 (MQ=255) tttGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCa < 1:1199080/139‑1 (MQ=255) cTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAAc > 1:340696/1‑139 (MQ=255) tCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACg > 2:66474/1‑139 (MQ=255) cGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTca < 2:340696/139‑1 (MQ=255) tCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGaa > 2:1682407/1‑130 (MQ=255) tCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGaa < 1:1682407/130‑1 (MQ=255) cGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGc > 2:1134468/1‑139 (MQ=255) cATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCAct < 2:174836/139‑1 (MQ=255) atatCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCActc < 2:1022903/139‑1 (MQ=255) tatCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAgcggcg < 2:225107/80‑1 (MQ=255) tatCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAgcggcg > 1:225107/1‑80 (MQ=255) ggCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTaa > 2:1543237/1‑139 (MQ=255) ggCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCg < 2:1265080/121‑1 (MQ=255) ggCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCg > 1:1265080/1‑121 (MQ=255) ggCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTaaa > 1:454420/1‑139 (MQ=255) ggCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTaaa > 1:143020/1‑139 (MQ=255) ggCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTaaa > 2:679338/1‑139 (MQ=255) gCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGaa > 1:785061/1‑107 (MQ=255) gCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGaa < 2:785061/107‑1 (MQ=255) tGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGgttgt < 1:1493106/83‑1 (MQ=255) tGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGgttgt > 1:1200506/1‑83 (MQ=255) tGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGgttgt < 2:1200506/83‑1 (MQ=255) tGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGgttgt > 2:1493106/1‑83 (MQ=255) tGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAg > 1:117417/1‑139 (MQ=255) aaTTGCCGCACCCAGTGGTGTATCGGCCTGGGCAAAAAGGATGGGCG‑AAAAAAAAATAGCGGCGCTGGTAAAGGGTTGTTCCACATGCCCCGGGCCGAAAGTCACAATGCCGGGCTCCACCCTTTTTAAAGCCAGCGGa < 2:1174142/139‑1 (MQ=255) aTGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGgttgt < 1:1443949/78‑1 (MQ=255) aTGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGgttgt > 2:1443949/1‑78 (MQ=255) cGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAggg > 1:1360572/1‑69 (MQ=255) cGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAggg < 2:1360572/69‑1 (MQ=255) cGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGaa < 2:993814/93‑1 (MQ=255) cGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGaa > 1:993814/1‑93 (MQ=255) cgcccATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCagcaag > 2:1276851/3‑139 (MQ=255) cACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAggg < 1:797001/67‑1 (MQ=255) cACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAggg > 2:797001/1‑67 (MQ=255) ccATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAg < 2:1141281/139‑1 (MQ=255) ccATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAg < 1:643476/139‑1 (MQ=255) ccATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAg < 1:1276851/139‑1 (MQ=255) ttGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTtc < 2:143020/139‑1 (MQ=255) aTCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTcaca < 2:1553132/85‑1 (MQ=255) aTCGCCCTGGGCAAACAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTcaca > 1:1553132/1‑85 (MQ=255) aCAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTa < 2:1414819/93‑1 (MQ=255) aCAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTa > 1:1414819/1‑93 (MQ=255) aCAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATcc > 1:1728615/1‑139 (MQ=255) cAGGATGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCg < 1:679338/139‑1 (MQ=255) aTGGGCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAATTCATCACGAAAGGTAACCAATCCGGCGt > 1:1171884/1‑139 (MQ=255) gCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGgttgt < 1:333085/35‑1 (MQ=255) gCG‑AAAAAAAATTAGCGGCGCTGGTAAAGGgttgt > 2:333085/1‑35 (MQ=255) | GTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCG‑GAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGT > NC_000913/2152740‑2153007 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |