Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,161,529 G→A Q22Q (CAG→CAA)  iceT → putative citrate/iron‑citrate/zinc‑citrate efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,161,5290GA100.0% 216.8 / NA 66Q22Q (CAG→CAA) iceTputative citrate/iron‑citrate/zinc‑citrate efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/34);  total (32/34)

TTTTTCGACCGTCTGCGGCTGCGTTTTTCGCGTAAACCTAAACAAACGGTAACCGAGTAAATGACAGATCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAGTCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGTTGCATATGCACATGGTCATTGTCTCTTATGTGCTGACCGTGGCGGTGATGCTGCCCGCCAGCGG  >  NC_000913/2161404‑2161660
                                                                                                                             |                                                                                                                                   
tttttCGACCGTCTGCGGCTGCGTTTTTCGCGTAAACCTAAACAAACGGTAACCGAGTAAATGACAGATCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGAcacca                                                                                                                        <  1:789389/139‑1 (MQ=255)
                     cGTTTTTCGCGTAAACCTAAACAAACGGTAACCGAGTAAATGACAGATCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAGCACCGCCCTTc                                                                                                   >  1:278883/1‑139 (MQ=255)
                                             acGGTAACCGAGTAAATGACAGATCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGc                                                                                                                                 >  2:1570289/1‑85 (MQ=255)
                                             acGGTAACCGAGTAAATGACAGATCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGc                                                                                                                                 <  1:1570289/85‑1 (MQ=255)
                                             acGGTAACCGAGTAAATGACAGATCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGacac                                                                                                                          <  1:945504/92‑1 (MQ=255)
                                             acGGTAACCGAGTAAATGACAGATCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGacac                                                                                                                          >  2:945504/1‑92 (MQ=255)
                                             acGGTAACCGAGTAAATGACAGATCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCa                                                                                      >  1:1118791/1‑128 (MQ=255)
                                             acGGTAACCGAGTAAATGACAGATCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCa                                                                                      <  2:1118791/128‑1 (MQ=255)
                                             acGGTAACCGAGTAAATGACAGATCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCgggg                                                                           >  1:1116030/1‑139 (MQ=255)
                                               ggTAACCGAGTAAATGACAGATCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCg                                                                                                                                  >  2:175815/1‑82 (MQ=255)
                                               ggTAACCGAGTAAATGACAGATCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCg                                                                                                                                  <  1:175815/82‑1 (MQ=255)
                                                       aGTAAATGACAGATCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGaaa                                                                        <  1:751606/132‑1 (MQ=255)
                                                       aGTAAATGACAGATCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGaaa                                                                        >  2:751606/1‑132 (MQ=255)
                                                       aGTAAATGACAGATCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGtt                                                                 <  2:1302473/139‑1 (MQ=255)
                                                             tGACAGATCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGt                                                                  >  1:1513343/1‑132 (MQ=255)
                                                             tGACAGATCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGt                                                                  <  2:1513343/132‑1 (MQ=255)
                                                              gACAGATCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCCTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGTTGCATATg                                                          <  2:1116030/139‑1 (MQ=255)
                                                                 aGATCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGTTGCatat                                                           <  2:251845/135‑1 (MQ=255)
                                                                 aGATCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGTTGCatat                                                           >  1:251845/1‑135 (MQ=255)
                                                                   aTCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGAcacca                                                                                                                        >  2:914312/1‑72 (MQ=255)
                                                                   aTCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGAcacca                                                                                                                        <  1:914312/72‑1 (MQ=255)
                                                                   aTCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGt                                                                                                                 >  1:679622/1‑79 (MQ=255)
                                                                   aTCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGt                                                                                                                 <  2:679622/79‑1 (MQ=255)
                                                                    tCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGa                                                                          <  2:560430/117‑1 (MQ=255)
                                                                    tCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGa                                                                          >  1:560430/1‑117 (MQ=255)
                                                                       tCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGcc                                                                                                       >  1:1584933/1‑85 (MQ=255)
                                                                       tCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGcc                                                                                                       <  2:1584933/85‑1 (MQ=255)
                                                                         ccGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGTTGCATATGCACATGGTCAt                                               <  2:676877/139‑1 (MQ=255)
                                                                              aGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACAcc                                                                                                          >  1:982090/1‑75 (MQ=255)
                                                                              aGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACAcc                                                                                                          <  2:982090/75‑1 (MQ=255)
                                                                              aGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGcc                                                                                                       <  1:1569754/78‑1 (MQ=255)
                                                                              aGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGcc                                                                                                       >  2:1569754/1‑78 (MQ=255)
                                                                                cACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGcc                                                                                                       >  2:359629/1‑76 (MQ=255)
                                                                                cACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGcc                                                                                                       <  1:359629/76‑1 (MQ=255)
                                                                                cACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCt                                                                                <  2:268630/99‑1 (MQ=255)
                                                                                cACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCt                                                                                >  1:268630/1‑99 (MQ=255)
                                                                                  cccGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTaaa                                                                                                              >  1:511421/1‑67 (MQ=255)
                                                                                  cccGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTaaa                                                                                                              <  2:511421/67‑1 (MQ=255)
                                                                                  cccGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCg                                                                   <  1:1580203/110‑1 (MQ=255)
                                                                                  cccGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCg                                                                   >  2:1580203/1‑110 (MQ=255)
                                                                                      ttGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGaaa                                                                        <  2:1415168/101‑1 (MQ=255)
                                                                                      ttGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGaaa                                                                        >  1:1415168/1‑101 (MQ=255)
                                                                                       tGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGc                                                                                                                                 <  1:168217/43‑1 (MQ=255)
                                                                                       tGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGc                                                                                                                                 >  2:168217/1‑43 (MQ=255)
                                                                                       tGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCg                                                                                                         <  2:793275/67‑1 (MQ=255)
                                                                                       tGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCg                                                                                                         >  1:793275/1‑67 (MQ=255)
                                                                                        ggCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGTTGCATATGCACATGGTCGTTGTCTCTTATGTGCt                                >  1:135627/1‑139 (MQ=255)
                                                                                                tggattgtggCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGcc                                                                                                       >  2:124704/1‑60 (MQ=255)
                                                                                                tggattgtggCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGcc                                                                                                       <  1:124704/60‑1 (MQ=255)
                                                                                                  gattgtggCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGt                                                                  >  1:1423534/1‑95 (MQ=255)
                                                                                                  gattgtggCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGt                                                                  <  2:1423534/95‑1 (MQ=255)
                                                                                                   attgtggCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGTTGCATATGCACATGGTCATTGTCTCTTATGTGCTGACCGTGGCgg                     >  1:1057361/1‑139 (MQ=255)
                                                                                                       tggCTTTCGGCTTCTTTATGCAATCGCTGGCCACCACCATCGTAAACACCGCCCt                                                                                                     <  1:1057250/55‑1 (MQ=255)
                                                                                                       tggCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCt                                                                                                     >  2:1057250/1‑55 (MQ=255)
                                                                                                       tggCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGt                                                                      <  2:611281/86‑1 (MQ=255)
                                                                                                       tggCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGt                                                                      >  1:611281/1‑86 (MQ=255)
                                                                                                       tggCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGTTGCATATGCACATGGTCAt                                               <  2:168727/109‑1 (MQ=255)
                                                                                                       tggCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGTTGCATATGCACATGGTCAt                                               >  1:168727/1‑109 (MQ=255)
                                                                                                       tggCTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGTTGCATATGCACATGGTCATTGTCTCTTATGTGCTGACCGTGGCGGTGAt                 >  1:1583812/1‑139 (MQ=255)
                                                                                                          cTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGTTGCATATGCACATGGTCa                                                <  2:1546590/105‑1 (MQ=255)
                                                                                                          cTTTCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGTTGCATATGCACATGGTCa                                                >  1:1546590/1‑105 (MQ=255)
                                                                                                            ttCGGCTTCTTTATGTAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGTTGCATATGCACATGGTCATTGTCTCTTATGTGCTGACCGTGGCGGTGAtgctgc            <  1:766811/139‑1 (MQ=255)
                                                                                                            ttCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGTTGCa                                                              <  2:1234061/89‑1 (MQ=255)
                                                                                                            ttCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGTTGCa                                                              >  1:1234061/1‑89 (MQ=255)
                                                                                                            ttCGGCTTCTTTATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGTTGCATATGCACATGGTCATTGTCTCTTATGTGCTGACCGTGGCGGTGAtgctgc            <  2:1057361/139‑1 (MQ=255)
                                                                                                                      ttATGCAATCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGTTGCATATGCACATGGTCATTGTCTCTTATGTGCTGACCGTGGCGGTGATGCTGCCCGCCAGCgg  <  2:135627/139‑1 (MQ=255)
                                                                                                                             |                                                                                                                                   
TTTTTCGACCGTCTGCGGCTGCGTTTTTCGCGTAAACCTAAACAAACGGTAACCGAGTAAATGACAGATCTTCCCGACAGCACCCGTTGGCAATTGTGGATTGTGGCTTTCGGCTTCTTTATGCAGTCGCTGGACACCACCATCGTAAACACCGCCCTTCCCTCAATGGCGCAAAGCCTCGGGGAAAGTCCGTTGCATATGCACATGGTCATTGTCTCTTATGTGCTGACCGTGGCGGTGATGCTGCCCGCCAGCGG  >  NC_000913/2161404‑2161660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑