Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	200,214	C→T	P763S (CCA→TCA)	bamA →	BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	200,214	0	C	T	100.0%	155.7 / NA	49	P763S (CCA→TCA)	bamA	BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/24);  total (25/24)

TCATCACCCCGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATCCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAAC  >  NC_000913/200080‑200342                                                                                                                                       |                                                                                                                                 tcatcaCCCCGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAg                                                                                                                              <  2:1320135/139‑1 (MQ=255)     caCCCCGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAat                                                                                                                          <  2:412451/139‑1 (MQ=255)           gACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGt                                                                                                                    <  2:1059864/139‑1 (MQ=255)                cGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTc                                                                                                               >  2:928269/1‑139 (MQ=255)                  tttATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTg                                                                                                             >  2:1669773/1‑139 (MQ=255)                           gATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCg                                                                                                    <  2:414151/139‑1 (MQ=255)                             tAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCa                                                                                                  <  1:1014564/139‑1 (MQ=255)                             tAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCa                                                                                                  <  2:437602/139‑1 (MQ=255)                             tAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATGCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCa                                                                                                  <  1:1076740/139‑1 (MQ=255)                                         cTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAg                                                                                                                              >  1:1397462/1‑99 (MQ=255)                                         cTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAg                                                                                                                              <  2:1397462/99‑1 (MQ=255)                                           cGGTTCGTACTTCCTTCTTCTGGGATATGTGTACCGTTTGGTATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTc                                                                                    >  2:1702032/1‑139 (MQ=255)                                           cGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTc                                                                                    >  1:31745/1‑139 (MQ=255)                                                     ttccttcTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTgggg                                                                          <  1:928269/139‑1 (MQ=255)                                                              ctGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGgtg                                                                 >  1:945842/1‑139 (MQ=255)                                                                 ggATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATgg                                                                                         <  2:1598411/112‑1 (MQ=255)                                                                 ggATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATgg                                                                                         >  1:1598411/1‑112 (MQ=255)                                                                    tatGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCa                                                                                                                            >  2:1454522/1‑74 (MQ=255)                                                                    tatGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCa                                                                                                                            <  1:1454522/74‑1 (MQ=255)                                                                      tGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATc                                                                                                     >  2:1459113/1‑95 (MQ=255)                                                                      tGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATc                                                                                                     <  1:1459113/95‑1 (MQ=255)                                                                      tGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTa                                                         >  1:122706/1‑139 (MQ=255)                                                                             cGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCa                                                                                                                            <  2:954622/65‑1 (MQ=255)                                                                             cGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCa                                                                                                                            >  1:954622/1‑65 (MQ=255)                                                                             cGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAg                                                  >  2:1282585/1‑139 (MQ=255)                                                                             cGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAg                                                  <  2:31745/139‑1 (MQ=255)                                                                                           aCTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGgtg                                                                 <  2:497932/110‑1 (MQ=255)                                                                                           aCTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGgtg                                                                 >  1:497932/1‑110 (MQ=255)                                                                                              gggATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTtctc                                                            >  1:104841/1‑112 (MQ=255)                                                                                              gggATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTtctc                                                            <  2:104841/112‑1 (MQ=255)                                                                                              gggATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGt                                     >  1:1377560/1‑135 (MQ=255)                                                                                              gggATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGt                                     <  2:1377560/135‑1 (MQ=255)                                                                                                 aTTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCa                                                                                                  <  1:305278/71‑1 (MQ=255)                                                                                                 aTTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCa                                                                                                  >  2:305278/1‑71 (MQ=255)                                                                                                         cAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAgg                      >  2:836251/1‑139 (MQ=255)                                                                                                                  ggATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGt             >  2:280048/1‑139 (MQ=255)                                                                                                                  ggATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGt             >  1:1287741/1‑139 (MQ=255)                                                                                                                   gATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGtt            >  2:26660/1‑139 (MQ=255)                                                                                                                   gATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGCGCCGTTGGTGTTCTCCTACGCCCAGGCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGtt            <  1:1702032/139‑1 (MQ=255)                                                                                                                     tatCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTcc          <  1:280048/139‑1 (MQ=255)                                                                                                                      atCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACaa                                                                                            <  1:437159/56‑1 (MQ=255)                                                                                                                      atCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACaa                                                                                            >  2:437159/1‑56 (MQ=255)                                                                                                                       tCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGc                                                                         >  1:1725246/1‑74 (MQ=255)                                                                                                                       tCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGc                                                                         <  2:1725246/74‑1 (MQ=255)                                                                                                                        ccGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGAt                                                                                       <  1:1247889/59‑1 (MQ=255)                                                                                                                        ccGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGAt                                                                                       >  2:1247889/1‑59 (MQ=255)                                                                                                                             cTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAAc  >  1:64604/1‑139 (MQ=255)                                                                                                                                 aGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGAt                                >  2:1621316/1‑105 (MQ=255)                                                                                                                                 aGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGAt                                <  1:1621316/105‑1 (MQ=255)                                                                                                                                       |                                                                                                                                 TCATCACCCCGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATCCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAAC  >  NC_000913/200080‑200342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑