Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,244,245	G→A	A112A (GCG→GCA)	yeiG →	S‑formylglutathione hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,244,245	0	G	A	100.0%	160.2 / NA	49	A112A (GCG→GCA)	yeiG	S‑formylglutathione hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/28);  total (21/28)

GCGGAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGG  >  NC_000913/2244117‑2244349                                                                                                                                 |                                                                                                         gcggAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTAcc                                                                                                <  1:330652/139‑1 (MQ=255)     aaCTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGAt                                                                                            <  2:555450/139‑1 (MQ=255)     aaCTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGAt                                                                                            <  1:1704829/139‑1 (MQ=255)               tGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTAcc                                                                                                <  2:157126/125‑1 (MQ=255)               tGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTAcc                                                                                                >  1:157126/1‑125 (MQ=255)                      gTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGAt                                                                                     <  1:120232/129‑1 (MQ=255)                      gTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGAt                                                                                     >  2:120232/1‑129 (MQ=255)                      gTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGcgcg                                                                           <  1:78736/139‑1 (MQ=255)                           gCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGaa                                                                      >  2:898440/1‑139 (MQ=255)                                    cAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACg                                                                                                       <  2:488110/97‑1 (MQ=255)                                    cAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACg                                                                                                       >  1:488110/1‑97 (MQ=255)                                      gCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGAt                                                                                            >  1:844376/1‑106 (MQ=255)                                      gCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGAt                                                                                            <  2:844376/106‑1 (MQ=255)                                           cgcgGCGAAAAGGTTGCCAACGACGATGGCTACGTTTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTc                                                      >  2:249358/1‑139 (MQ=255)                                              ggcgAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGAt                                                                                            >  2:297302/1‑98 (MQ=255)                                              ggcgAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGAt                                                                                            <  1:297302/98‑1 (MQ=255)                                                cgAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGgc                                                              >  1:474616/1‑126 (MQ=255)                                                cgAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGgc                                                              <  2:474616/126‑1 (MQ=255)                                                               cgacgaTGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGc                                  <  1:249358/139‑1 (MQ=255)                                                                         tACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTgcg                        <  2:1505868/139‑1 (MQ=255)                                                                          aCGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTa                                                                   >  1:1279148/1‑95 (MQ=255)                                                                          aCGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTa                                                                   <  2:1279148/95‑1 (MQ=255)                                                                          aCGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTgcgc                       >  1:544296/1‑139 (MQ=255)                                                                            gATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGc                                                                                                      >  1:556131/1‑58 (MQ=255)                                                                            gATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGc                                                                                                      <  2:556131/58‑1 (MQ=255)                                                                            gATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTgcgc                                                                            <  1:1309716/84‑1 (MQ=255)                                                                            gATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTgcgc                                                                            >  2:1309716/1‑84 (MQ=255)                                                                                  ggCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGAt                                                                         >  1:1343489/1‑81 (MQ=255)                                                                                  ggCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGAt                                                                         <  2:1343489/81‑1 (MQ=255)                                                                                    ccAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGt             <  2:544296/139‑1 (MQ=255)                                                                                    ccAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGt             <  1:898440/139‑1 (MQ=255)                                                                                    ccAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGt             <  1:634484/139‑1 (MQ=255)                                                                                      aGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTc                                                  <  2:1650859/100‑1 (MQ=255)                                                                                      aGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTc                                                  >  1:1650859/1‑100 (MQ=255)                                                                                        ggCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGAt                                                                                            >  1:1280435/1‑56 (MQ=255)                                                                                        ggCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGAt                                                                                            <  2:1280435/56‑1 (MQ=255)                                                                                        ggCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGa                                                                       <  1:857152/77‑1 (MQ=255)                                                                                        ggCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGa                                                                       >  2:857152/1‑77 (MQ=255)                                                                                          cgcAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCa       <  1:1079346/139‑1 (MQ=255)                                                                                                           aTGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCg                             >  1:1049174/1‑100 (MQ=255)                                                                                                           aTGCCACGCAACCGCCGTGGACAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCg                             <  2:1049174/100‑1 (MQ=255)                                                                                                             gCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCaa      >  2:128349/1‑121 (MQ=255)                                                                                                             gCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCaa      <  1:128349/121‑1 (MQ=255)                                                                                                                 cGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTc                                                  >  1:654491/1‑73 (MQ=255)                                                                                                                 cGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTc                                                  <  2:654491/73‑1 (MQ=255)                                                                                                                 cGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTa                                          <  1:736491/81‑1 (MQ=255)                                                                                                                 cGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTa                                          >  2:736491/1‑81 (MQ=255)                                                                                                                           gTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATggg  >  2:1682427/1‑111 (MQ=255)                                                                                                                           gTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATggg  <  1:1682427/111‑1 (MQ=255)                                                                                                                                 |                                                                                                         GCGGAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGG  >  NC_000913/2244117‑2244349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑