| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,276,530 | G→A | S77S (TCG→TCA) | yejF → | microcin C ABC transporter ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,276,530 | 0 | G | A | 96.5% | 171.4 / ‑1.7 | 57 | S77S (TCG→TCA) | yejF | microcin C ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (25/30); total (26/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.22e-01 | |||||||||||
AGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCGCTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTAAATCCATTGCATACCCTGGAAAAACAGCTTTATGAAGTGCTTTCACT > NC_000913/2276397‑2276661 | aGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCactgct < 2:589790/139‑1 (MQ=255) cTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCa > 2:1165755/1‑139 (MQ=255) ggCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACg > 1:236490/1‑139 (MQ=255) gCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGc > 2:1458324/1‑139 (MQ=255) cGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGcc < 2:236490/139‑1 (MQ=255) ggcgctggTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCaa > 2:937807/1‑139 (MQ=255) tgggtgAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTgcg < 1:878886/139‑1 (MQ=255) tgggtgAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTgcg < 1:937807/139‑1 (MQ=255) cAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAcg < 1:1715865/139‑1 (MQ=255) gTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCgg > 1:5819/1‑139 (MQ=255) gCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGtaataa > 1:1524674/1‑139 (MQ=255) aaGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGcc > 2:1643713/1‑100 (MQ=255) aaGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGcc < 1:1643713/100‑1 (MQ=255) gcTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGAttttt < 1:1458324/139‑1 (MQ=255) tGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCa < 2:1027755/139‑1 (MQ=255) tCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAgg < 2:343130/139‑1 (MQ=255) gCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAcg > 2:782115/1‑100 (MQ=255) gCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAcg < 1:782115/100‑1 (MQ=255) gCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGgt > 1:846208/1‑139 (MQ=255) ccTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGgtg < 1:251943/139‑1 (MQ=255) cTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAcg < 2:1452673/98‑1 (MQ=255) cTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAcg > 1:1452673/1‑98 (MQ=255) cTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGAttttt < 1:997995/123‑1 (MQ=255) cTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGAttttt > 2:997995/1‑123 (MQ=255) gCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATc > 1:880510/1‑77 (MQ=255) gCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATc < 2:880510/77‑1 (MQ=255) gCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCg > 2:436325/1‑139 (MQ=255) cTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGt > 2:465382/1‑139 (MQ=255) ttCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGgtg > 1:1155066/1‑130 (MQ=255) ttCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCATCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGgtg < 2:1155066/130‑1 (MQ=255) ccccGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGAt < 1:1459071/100‑1 (MQ=255) ccccGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGAt > 2:1459071/1‑100 (MQ=255) cccGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTAAATCCa < 2:1062003/139‑1 (MQ=255) ccgGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGATTCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTAAATCCATTGc < 2:1038206/139‑1 (MQ=255) ccgGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCACGAACCGATGGTGTCGTTAAATCCATTGc > 1:1038206/1‑139 (MQ=255) ggTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCGCTGCTTCACGCCAGCGATCAAACGtt < 1:725742/69‑1 (MQ=255) ggTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCGCTGCTTCACGCCAGCGATCAAACGtt > 2:725742/1‑69 (MQ=255) gTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCa < 2:1552293/107‑1 (MQ=255) gTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCa > 1:1552293/1‑107 (MQ=255) gAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTgcgc > 1:1414132/1‑69 (MQ=255) gAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTgcgc < 2:1414132/69‑1 (MQ=255) ctcCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAcg > 1:783739/1‑69 (MQ=255) ctcCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAcg < 2:783739/69‑1 (MQ=255) ctcCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTAAATCCATTGCATACCCTGGaaaaa < 2:73484/139‑1 (MQ=255) ctcCGGCGATATTCGTTTGCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTAAATCCATTGCATACCCTGGaaaaa < 1:1165755/139‑1 (MQ=255) cGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGAt < 1:1326037/87‑1 (MQ=255) cGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGAt > 2:1326037/1‑87 (MQ=255) gCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTAAATCCATTGCATACCCTGGAAAAACAGCt < 1:436325/139‑1 (MQ=255) cGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGgt > 1:79623/1‑102 (MQ=255) cGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGgt < 2:79623/102‑1 (MQ=255) ttCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAcg > 1:385327/1‑58 (MQ=255) ttCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAcg < 2:385327/58‑1 (MQ=255) tGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTaa > 2:476476/1‑95 (MQ=255) tGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTaa < 1:476476/95‑1 (MQ=255) ggCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAc > 2:903831/1‑46 (MQ=255) ggCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAc < 1:903831/46‑1 (MQ=255) gCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTAAATCCATTGCATACCCTGGAAAAACAGCTTTATGAAGTGCTTTCACt < 1:465382/139‑1 (MQ=255) gCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTAAATCCATTGCATACCCTGGAAAAACAGCTTTATGAAGTGCTTTCACt < 2:5819/139‑1 (MQ=255) | AGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCGCTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTAAATCCATTGCATACCCTGGAAAAACAGCTTTATGAAGTGCTTTCACT > NC_000913/2276397‑2276661 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |