Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,478,092	A→G	V82V (GTA→GTG)	dsdX →	D‑serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,478,092	0	A	G	100.0%	230.2 / NA	64	V82V (GTA→GTG)	dsdX	D‑serine transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/37);  total (27/37)

GTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCG  >  NC_000913/2477964‑2478210                                                                                                                                 |                                                                                                                       gTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCg                                                                                                              <  2:1014140/139‑1 (MQ=255)     gAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCaga                                                                                                          <  1:563636/139‑1 (MQ=255)        cGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCagaaag                                                                                                       >  1:597787/1‑139 (MQ=255)         gatgatGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCagaaaga                                                                                                      <  2:1408264/139‑1 (MQ=255)                 gCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCt                                                                                              <  2:67892/139‑1 (MQ=255)                  cATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCgggg                                                                                                                 >  2:436710/1‑119 (MQ=255)                  cATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCgggg                                                                                                                 <  1:436710/119‑1 (MQ=255)                     ggggCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGaca                                                                                          >  1:1254441/1‑139 (MQ=255)                           aCTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCaa                                                                                    >  2:805029/1‑139 (MQ=255)                            cTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCg                                                                                                              <  1:863093/112‑1 (MQ=255)                            cTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCg                                                                                                              >  2:863093/1‑112 (MQ=255)                                   tGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCg                                                                            <  1:805029/139‑1 (MQ=255)                                   tGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCg                                                                            <  1:424800/139‑1 (MQ=255)                                    ggTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGc                                                                           <  1:591248/139‑1 (MQ=255)                                         aTGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCgggg                                                                                                                 >  2:1320210/1‑96 (MQ=255)                                         aTGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCgggg                                                                                                                 <  1:1320210/96‑1 (MQ=255)                                         aTGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCt                                                                      >  2:1346831/1‑139 (MQ=255)                                               ttGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGt                                                                >  1:1528489/1‑139 (MQ=255)                                                  aaaGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGa                                                             <  2:1528489/139‑1 (MQ=255)                                                      tGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCt                                                                          >  2:318858/1‑122 (MQ=255)                                                      tGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTc                                                         >  2:670866/1‑139 (MQ=255)                                                      tGGAATTGGCGGAACGCTGGGGGTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCt                                                                          <  1:318858/122‑1 (MQ=255)                                                       ggAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCa                                                        >  2:1019600/1‑139 (MQ=255)                                                        gAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTTCCGCTGGCTTTCAGTTGATGTCAt                                                       <  1:1449030/139‑1 (MQ=255)                                                        gAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCAt                                                       <  1:306622/139‑1 (MQ=255)                                                          aTTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTa                                                     >  2:1067006/1‑139 (MQ=255)                                                             ggCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATgg                                                  >  1:37647/1‑139 (MQ=255)                                                                   aCGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTgg                                            <  2:1449779/139‑1 (MQ=255)                                                                   aCGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTgg                                            <  2:597787/139‑1 (MQ=255)                                                                   aCGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTgg                                            >  2:1163086/1‑139 (MQ=255)                                                                               cTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGa                                                                                            >  2:349877/1‑79 (MQ=255)                                                                               cTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGa                                                                                            <  1:349877/79‑1 (MQ=255)                                                                               cTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTa                                                     <  1:896714/118‑1 (MQ=255)                                                                               cTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTa                                                     >  2:896714/1‑118 (MQ=255)                                                                                tCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTtg                               <  2:301337/139‑1 (MQ=255)                                                                                 cGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGa                                                                                            >  1:684842/1‑77 (MQ=255)                                                                                 cGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGa                                                                                            <  2:684842/77‑1 (MQ=255)                                                                                  gCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTtgtg                             <  1:670866/139‑1 (MQ=255)                                                                                     gCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCa                          <  1:385024/139‑1 (MQ=255)                                                                                         ttATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCAc                      >  2:1110627/1‑139 (MQ=255)                                                                                          tATCGGCCTTGGCACGATACTGGGAAAAATGATGTAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                     <  1:1110627/139‑1 (MQ=255)                                                                                          tATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                     <  1:1067006/139‑1 (MQ=255)                                                                                            tCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCt                   <  1:1163086/139‑1 (MQ=255)                                                                                                 cTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCtgtttg              <  1:851889/139‑1 (MQ=255)                                                                                                   tGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCaga                                                                                                          <  2:1598457/45‑1 (MQ=255)                                                                                                   tGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCaga                                                                                                          >  1:1598457/1‑45 (MQ=255)                                                                                                   tGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGc                                                                           <  1:1566360/76‑1 (MQ=255)                                                                                                   tGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGc                                                                           >  2:1566360/1‑76 (MQ=255)                                                                                                   tGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTtgtg                             >  1:1694038/1‑122 (MQ=255)                                                                                                   tGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTtgtg                             <  2:1694038/122‑1 (MQ=255)                                                                                                     gCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGc                                                                           <  2:276128/74‑1 (MQ=255)                                                                                                     gCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGc                                                                           >  1:276128/1‑74 (MQ=255)                                                                                                       aCGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAg        <  2:1254441/139‑1 (MQ=255)                                                                                                          aTACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTg                                                                                                  >  2:381761/1‑46 (MQ=255)                                                                                                          aTACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTg                                                                                                  <  1:381761/46‑1 (MQ=255)                                                                                                             cTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCACTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGcg  <  1:1019600/139‑1 (MQ=255)                                                                                                              tGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                     >  2:1100383/1‑119 (MQ=255)                                                                                                              tGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                     <  1:1100383/119‑1 (MQ=255)                                                                                                                   aaaaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGcg  >  2:202259/1‑133 (MQ=255)                                                                                                                   aaaaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGcg  <  1:202259/133‑1 (MQ=255)                                                                                                                     aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCttt                                                                    >  2:941309/1‑65 (MQ=255)                                                                                                                     aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCttt                                                                    <  1:941309/65‑1 (MQ=255)                                                                                                                     aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                     <  2:1588629/112‑1 (MQ=255)                                                                                                                     aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                     >  1:1588629/1‑112 (MQ=255)                                                                                                                                 |                                                                                                                       GTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCG  >  NC_000913/2477964‑2478210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑