Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,494,950	T→C	Y85H (TAT→CAT)	ypdI →	putative lipoprotein involved in colanic acid biosynthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,494,950	0	T	C	100.0%	254.5 / NA	71	Y85H (TAT→CAT)	ypdI	putative lipoprotein involved in colanic acid biosynthesis
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/40);  total (31/40)

AAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAG  >  NC_000913/2494814‑2495077                                                                                                                                         |                                                                                                                                aaaaaCATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCAt                                                                                                                               <  2:750726/139‑1 (MQ=255)          aGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCAt                                                                                                                               >  2:178713/1‑130 (MQ=255)                 acgacCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCAt                                                                                                                               >  1:551689/1‑123 (MQ=255)                 acgacCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCAt                                                                                                                               <  2:551689/123‑1 (MQ=255)                  cgacCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCg                                                                                                              <  2:872745/139‑1 (MQ=255)                             tctACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCAt                                                                                                                               >  1:406156/1‑111 (MQ=255)                             tctACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCAt                                                                                                                               <  2:406156/111‑1 (MQ=255)                             tctACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCttt                                                                                                   <  1:58998/139‑1 (MQ=255)                               tACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATaa                                                                                                          <  1:1384575/130‑2 (MQ=255)                               tACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATaa                                                                                                          >  2:1384575/1‑129 (MQ=255)                                     aCTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGc                                                                                           <  2:937977/139‑1 (MQ=255)                                     aCTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGc                                                                                           <  2:1152317/139‑1 (MQ=255)                                       ttCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCCCAGAAGAAATGCCGCACCCTTTTCCGACAAAGGCGAATCATCTTATTCAACCTGTGCGATAACGTCTTTCAAATAGCAg                                                                                         >  2:278778/1‑139 (MQ=255)                                       ttCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAg                                                                                         <  2:1205151/139‑1 (MQ=255)                                           gCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGa                                                                                     <  1:1466786/139‑1 (MQ=255)                                            ccATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGaa                                                                                    <  2:1072568/139‑1 (MQ=255)                                                 acaGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATc                                                                               <  1:278778/139‑1 (MQ=255)                                                      cGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTc                                                                          <  2:1525951/139‑1 (MQ=255)                                                         ttttGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctga                                                                       <  1:737571/139‑1 (MQ=255)                                                          tttGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctgat                                                                      >  1:708505/1‑139 (MQ=255)                                                              tttAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCAt                                                                                                                               <  1:173764/78‑1 (MQ=255)                                                                        gagaAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTg                                                                                      <  2:1378992/109‑1 (MQ=255)                                                                        gagaAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTg                                                                                      >  1:1378992/1‑109 (MQ=255)                                                                           aagaTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTg                                                     >  1:337629/1‑139 (MQ=255)                                                                              aTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCt                                                      >  2:1021728/1‑135 (MQ=255)                                                                              aTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCt                                                      <  1:1021728/135‑1 (MQ=255)                                                                                 cgcCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCAt                                                                                                                               <  1:672306/59‑1 (MQ=255)                                                                                 cgcCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATg                                                                            <  2:1329419/110‑1 (MQ=255)                                                                                 cgcCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATg                                                                            >  1:1329419/1‑110 (MQ=255)                                                                                   ccaccaTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATa                                                                                                           <  2:1416545/77‑1 (MQ=255)                                                                                   ccaccaTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATa                                                                                                           >  1:1416545/1‑77 (MQ=255)                                                                                   ccaccaTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACaa                                                                                 <  2:117272/103‑1 (MQ=255)                                                                                   ccaccaTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACaa                                                                                 >  1:117272/1‑103 (MQ=255)                                                                                   ccaccaTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATg                                             >  1:617528/1‑139 (MQ=255)                                                                                   ccaccaTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATg                                             >  1:944080/1‑139 (MQ=255)                                                                                      ccaTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCa                                                                                          >  2:980900/1‑91 (MQ=255)                                                                                      ccaTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCa                                                                                          <  1:980900/91‑1 (MQ=255)                                                                                       caTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGa                                                                                     >  2:1194582/1‑95 (MQ=255)                                                                                       caTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGa                                                                                     <  1:1194582/95‑1 (MQ=255)                                                                                       caTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCACAAATGAGcc                                         >  2:1092678/1‑139 (MQ=255)                                                                                           aaaCCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATcc                                     <  1:572862/139‑1 (MQ=255)                                                                                               cTCACAGAAGAAATGCCGCCCTCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAAACATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGAc                                 <  1:1092678/139‑1 (MQ=255)                                                                                               cTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGAc                                 <  1:885119/139‑1 (MQ=255)                                                                                                 cacaGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTAt                                                                                                                          >  2:1074380/1‑48 (MQ=255)                                                                                                 cacaGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTAt                                                                                                                          <  1:1074380/48‑1 (MQ=255)                                                                                                 cacaGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGt                                                                                                       <  2:150391/67‑1 (MQ=255)                                                                                                 cacaGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGt                                                                                                       >  1:150391/1‑67 (MQ=255)                                                                                                 cacaGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGa                                                                                     >  2:1520176/1‑85 (MQ=255)                                                                                                 cacaGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGa                                                                                     <  1:1520176/85‑1 (MQ=255)                                                                                                 cacaGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAg                                           >  2:1603924/1‑127 (MQ=255)                                                                                                 cacaGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAg                                           <  1:1603924/127‑1 (MQ=255)                                                                                                 cacaGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGAccc                               >  2:1520941/1‑139 (MQ=255)                                                                                                      aagaaATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTc                                                                                                      <  1:892769/63‑1 (MQ=255)                                                                                                      aagaaATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTc                                                                                                      >  2:892769/1‑63 (MQ=255)                                                                                                         aaaTGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGc                       <  2:337629/139‑1 (MQ=255)                                                                                                                gccCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATaa                                                                                                          >  1:205334/1‑48 (MQ=255)                                                                                                                gccCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATaa                                                                                                          <  2:205334/49‑2 (MQ=255)                                                                                                                 cccccTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATa               <  1:1520941/139‑1 (MQ=255)                                                                                                                 cccccTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATa               <  2:708505/139‑1 (MQ=255)                                                                                                                   cccTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTTAACAATc                                                                               <  1:1456609/73‑1 (MQ=255)                                                                                                                   cccTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTTAACAATc                                                                               >  2:1456609/1‑73 (MQ=255)                                                                                                                   cccTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctgatgct                                                                   >  1:376011/1‑85 (MQ=255)                                                                                                                   cccTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctgatgct                                                                   <  2:376011/85‑1 (MQ=255)                                                                                                                       tttCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCccac         >  1:616797/1‑139 (MQ=255)                                                                                                                              cAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACAt                                                                                              <  2:332548/47‑1 (MQ=38)                                                                                                                              cAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACAt                                                                                              >  1:332548/1‑47 (MQ=38)                                                                                                                              cAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATAcccc           <  2:1713109/130‑1 (MQ=255)                                                                                                                              cAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATAcccc           >  1:1713109/1‑130 (MQ=255)                                                                                                                              cAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAg  >  2:208685/1‑139 (MQ=255)                                                                                                                                 aGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTg                                                     <  2:62713/85‑1 (MQ=255)                                                                                                                                 aGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTg                                                     >  1:62713/1‑85 (MQ=255)                                                                                                                                         |                                                                                                                                AAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAG  >  NC_000913/2494814‑2495077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑