| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,510,111 | G→A | G161D (GGC→GAC) | yfeO → | putative ion channel protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,510,111 | 0 | G | A | 92.1% | 96.7 / 1.4 | 38 | G161D (GGC→GAC) | yfeO | putative ion channel protein |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (19/16); total (21/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01 | |||||||||||
CCTGGGGCCGGAACATCCGATCATGACCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGGCACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCG > NC_000913/2509977‑2510230 | ccTGGGGCCGGAACATCCGATCATGACCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACt > 2:1321210/1‑139 (MQ=255) aCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATAtttt > 1:1521721/1‑139 (MQ=255) tCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCa > 1:838302/1‑139 (MQ=255) gCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCa > 2:829887/1‑139 (MQ=255) gCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTgcggcg < 2:656984/102‑1 (MQ=255) gCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTgcggcg > 1:656984/1‑102 (MQ=255) gCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCaaa > 2:429984/1‑121 (MQ=255) gCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCaaa < 1:429984/121‑1 (MQ=255) gCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTg > 2:1279983/1‑139 (MQ=255) gCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTg > 1:933102/1‑139 (MQ=255) tGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGtt < 2:1707242/139‑1 (MQ=255) ggCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTc < 1:1279983/139‑1 (MQ=255) ggCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTc < 2:933102/139‑1 (MQ=255) gcgcgTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTc > 2:408804/1‑122 (MQ=255) gcgcgTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTc < 1:408804/122‑1 (MQ=255) gcgcgTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGtctc > 1:1224729/1‑139 (MQ=255) cgTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAAt < 1:1676751/103‑1 (MQ=255) cgTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAAt > 2:1676751/1‑103 (MQ=255) gTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTg < 1:1321210/139‑1 (MQ=255) aaCCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTgcgc < 1:829887/139‑1 (MQ=255) tGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATgg < 1:715835/94‑1 (MQ=255) tGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATgg > 2:715835/1‑94 (MQ=255) tttAGCCTCTGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCt > 1:1279695/1‑139 (MQ=255) tGCCGGAACCATCGGCGCACTGTTTGGCACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACtt > 1:273387/1‑139 (MQ=255) tGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCa > 2:375/1‑60 (MQ=255) tGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCa < 1:375/60‑1 (MQ=255) tGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTgc < 2:1316523/108‑1 (MQ=255) tGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTgc > 1:1316523/1‑108 (MQ=255) tGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATggcgg < 1:998906/122‑1 (MQ=255) tGCCGGAACCATCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATggcgg > 2:998906/1‑122 (MQ=255) aTCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTgcg > 2:261058/1‑99 (MQ=255) aTCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTgcg < 1:261058/99‑1 (MQ=255) aTCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTg < 1:915002/124‑1 (MQ=255) aTCGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTg > 2:915002/1‑124 (MQ=255) tCGGCGCACTGTTTGGCACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCg < 2:279836/135‑1 (MQ=255) tCGGCGCACTGTTTGGCACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCg > 1:279836/1‑135 (MQ=255) cGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGc < 2:1317447/47‑1 (MQ=255) cGGCGCACTGTTTGACACTCCTGTTGCGGCGGCGTTGATATTTTCGc > 1:1317447/1‑47 (MQ=255) | CCTGGGGCCGGAACATCCGATCATGACCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGGCACTCCTGTTGCGGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCG > NC_000913/2509977‑2510230 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |