Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,589,535 C→T I647I (ATC→ATT)  acrD → aminoglycoside/multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,589,5350CT100.0% 128.7 / NA 40I647I (ATC→ATT) acrDaminoglycoside/multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/21);  total (19/21)

AAGACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCA  >  NC_000913/2589399‑2589651
                                                                                                                                        |                                                                                                                    
aaGACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGa                                                                                                                    <  2:1542428/139‑1 (MQ=255)
 aGACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAg                                                                                                                   <  1:422466/139‑1 (MQ=255)
                      tGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAAc                                                                                              <  2:109020/139‑1 (MQ=255)
                       gCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAAcc                                                                                             >  1:709118/1‑139 (MQ=255)
                        ccACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCa                                                                                            <  2:539820/139‑1 (MQ=255)
                                 gTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAaaga                                                                                   >  2:881593/1‑139 (MQ=255)
                                      ggCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGTGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAg                                                                                                                   <  2:1227549/102‑1 (MQ=255)
                                      ggCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAg                                                                                                                   >  1:1227549/1‑102 (MQ=255)
                                        cccTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAAc                                                                                              <  1:100709/121‑1 (MQ=255)
                                        cccTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAAc                                                                                              >  2:100709/1‑121 (MQ=255)
                                                   aCGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCcag                                                                 <  1:1339265/139‑1 (MQ=255)
                                                                ggCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTcgcg                                                                           <  2:1510084/116‑1 (MQ=255)
                                                                ggCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTcgcg                                                                           >  1:1510084/1‑116 (MQ=255)
                                                                                   cTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCg                                                                                                                 >  2:1253843/1‑59 (MQ=255)
                                                                                   cTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCg                                                                                                                 <  1:1253843/59‑1 (MQ=255)
                                                                                   cTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAAc                                                                                              <  2:552063/78‑1 (MQ=255)
                                                                                   cTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAAc                                                                                              >  1:552063/1‑78 (MQ=255)
                                                                                   cTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGtt                                 >  1:1322883/1‑139 (MQ=255)
                                                                                    tGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTc                                >  1:1646997/1‑139 (MQ=255)
                                                                                            tGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGtt                        <  2:1322883/139‑1 (MQ=255)
                                                                                             ggAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAg                                               >  2:1679601/1‑115 (MQ=255)
                                                                                             ggAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAg                                               <  1:1679601/115‑1 (MQ=255)
                                                                                             ggAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGttt                       <  2:709118/139‑1 (MQ=255)
                                                                                                 cGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTc                                                                              >  1:1277574/1‑80 (MQ=255)
                                                                                                 cGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTc                                                                              <  2:1277574/80‑1 (MQ=255)
                                                                                                         aCAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTcgcg                                                                           <  1:1598654/75‑1 (MQ=255)
                                                                                                         aCAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTcgcg                                                                           >  2:1598654/1‑75 (MQ=255)
                                                                                                                  ccGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCa  >  2:1179623/1‑139 (MQ=255)
                                                                                                                    ggCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAAc                                                                                              >  2:152026/1‑45 (MQ=255)
                                                                                                                    ggCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAAc                                                                                              <  1:152026/45‑1 (MQ=255)
                                                                                                                       aCCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGc         >  2:608810/1‑127 (MQ=255)
                                                                                                                       aCCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGc         <  1:608810/127‑1 (MQ=255)
                                                                                                                           cGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAg                                               >  2:381874/1‑85 (MQ=255)
                                                                                                                           cGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAg                                               <  1:381874/85‑1 (MQ=255)
                                                                                                                           cGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTgg                                      >  1:185438/1‑94 (MQ=255)
                                                                                                                           cGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTgg                                      <  2:185438/94‑1 (MQ=255)
                                                                                                                                gCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGc                                                         >  2:159621/1‑70 (MQ=255)
                                                                                                                                gCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGc                                                         <  1:159621/70‑1 (MQ=255)
                                                                                                                                     tATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGTCCGCCAGCAATTAGCg                                             <  1:842515/77‑1 (MQ=255)
                                                                                                                                     tATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGTCCGCCAGCAATTAGCg                                             >  2:842515/1‑77 (MQ=255)
                                                                                                                                        |                                                                                                                    
AAGACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCA  >  NC_000913/2589399‑2589651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑