Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,697,915	C→T	*212* (TAG→TAA)	pgpC ←	phosphatidylglycerophosphatase C, membrane bound

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,697,915	0	C	T	100.0%	152.3 / NA	47	*212* (TAG→TAA)	pgpC	phosphatidylglycerophosphatase C, membrane bound
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/23);  total (24/23)

ATCCCAGGCACGTTTCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCA  >  NC_000913/2697786‑2698048                                                                                                                                  |                                                                                                                                      aTCCCAGGCACGTTTCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGt                                                                                                                              <  2:289920/139‑1 (MQ=255)             tttCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTg                                                                                                                         >  2:1655752/1‑132 (MQ=255)             tttCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTg                                                                                                                         <  1:1655752/132‑1 (MQ=255)             tttCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCa                                                                                                                  >  2:811451/1‑139 (MQ=255)             tttCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATAAACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCa                                                                                                                  >  1:1696206/1‑139 (MQ=255)             tttCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTAGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCa                                                                                                                  >  2:1047631/1‑139 (MQ=255)                        tCAGCGCTTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGt                                                                                                       <  1:811451/139‑1 (MQ=255)                          aGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTa                                                                                                     <  2:1696206/139‑1 (MQ=255)                                 gACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACcgc                                                                                                              <  1:743518/123‑1 (MQ=255)                                 gACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACcgc                                                                                                              >  2:743518/1‑123 (MQ=255)                                  aCGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCaa                                                                                             >  2:1479225/1‑139 (MQ=255)                                              ttCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAg                                                                                 >  2:363558/1‑139 (MQ=255)                                                 gTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCa                                                                                                                  <  2:1436978/103‑1 (MQ=255)                                                 gTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCa                                                                                                                  >  1:1436978/1‑103 (MQ=255)                                                  tGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAAt                                                                             >  1:1698648/1‑139 (MQ=255)                                                            cGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCgg                                                                   <  1:1479225/139‑1 (MQ=255)                                                                 tCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGTCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGt                                                              <  1:1047631/139‑1 (MQ=255)                                                                      cAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGa                                                         <  1:363558/139‑1 (MQ=255)                                                                                    cGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCa                                                                                                                                >  1:595504/1‑54 (MQ=255)                                                                                    cGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCa                                                                                                                                <  2:595504/54‑1 (MQ=255)                                                                                     ggTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTa                                                                                                     <  2:135516/80‑1 (MQ=255)                                                                                     ggTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTa                                                                                                     >  1:135516/1‑80 (MQ=255)                                                                                      gTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGc                                         <  2:1698648/139‑1 (MQ=255)                                                                                                 gcggcgCATTATACACGGACGCTATGCTTTACTTTATTTCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCAc                                      >  2:368401/1‑131 (MQ=255)                                                                                                 gcggcgCATTATACACGGACGCTATGCTTTACTTTATTTCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCAc                                      <  1:368401/131‑1 (MQ=255)                                                                                                 gcggcgCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCa                                                                                              <  2:1595259/75‑1 (MQ=255)                                                                                                 gcggcgCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCa                                                                                              >  1:1595259/1‑75 (MQ=255)                                                                                                 gcggcgCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGAtt                                                                >  1:388349/1‑105 (MQ=255)                                                                                                 gcggcgCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGAtt                                                                <  2:388349/105‑1 (MQ=255)                                                                                                 gcggcgCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACt                                                    <  2:1549002/117‑1 (MQ=255)                                                                                                 gcggcgCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACt                                                    >  1:1549002/1‑117 (MQ=255)                                                                                                          tataCACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATa                                                                            >  1:1520981/1‑84 (MQ=255)                                                                                                          tataCACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATa                                                                            <  2:1520981/84‑1 (MQ=255)                                                                                                                  gACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGAt             >  1:945255/1‑139 (MQ=255)                                                                                                                    cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCAc                                                                                                                 >  1:1646508/1‑37 (MQ=255)                                                                                                                    cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCAc                                                                                                                 <  2:1646508/37‑1 (MQ=255)                                                                                                                    cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATg                                                                                        <  1:1114692/62‑1 (MQ=255)                                                                                                                    cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATg                                                                                        >  2:1114692/1‑62 (MQ=255)                                                                                                                    cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGa                                                                                    <  2:1514224/66‑1 (MQ=255)                                                                                                                    cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGa                                                                                    >  2:1245249/1‑66 (MQ=255)                                                                                                                    cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGa                                                                                    >  1:1514224/1‑66 (MQ=255)                                                                                                                    cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGa                                                                                    <  1:1245249/66‑1 (MQ=255)                                                                                                                    cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGAt                                                        >  2:1511691/1‑94 (MQ=255)                                                                                                                    cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGAt                                                        <  1:1511691/94‑1 (MQ=255)                                                                                                                    cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGAttt           >  2:166241/1‑139 (MQ=255)                                                                                                                       tATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTgc        >  2:1401836/1‑139 (MQ=255)                                                                                                                           ctttactttaTTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTc    >  2:870832/1‑139 (MQ=255)                                                                                                                             ttACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCa  <  1:1401836/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                      ATCCCAGGCACGTTTCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCA  >  NC_000913/2697786‑2698048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑