| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,721,193 | C→T | A414V (GCA→GTA) | pka → | protein lysine acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,721,193 | 0 | C | T | 98.2% | 182.1 / ‑6.8 | 57 | A414V (GCA→GTA) | pka | protein lysine acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (26/30); minor base A (0/1); total (26/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCCAGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCC > NC_000913/2721068‑2721320 | gCCCAGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCGGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCCCAAGAGGTACGACGTTTATTc > 2:1012137/1‑139 (MQ=255) cgcTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGcc < 1:1708662/139‑1 (MQ=255) ctgctCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCgg > 2:1461802/1‑139 (MQ=255) gctCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGc < 1:440167/139‑1 (MQ=255) tCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTg < 1:1012137/139‑1 (MQ=255) cccGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGc > 2:191704/1‑139 (MQ=255) cccGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGc > 2:1308217/1‑139 (MQ=255) aGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTAc > 1:718029/1‑139 (MQ=255) aTTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCggaagga < 2:1166730/139‑1 (MQ=255) aaGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGAc > 2:863813/1‑112 (MQ=255) aaGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGAc < 1:863813/112‑1 (MQ=255) aGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGTACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACt > 2:1013831/1‑139 (MQ=255) gCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTg > 1:1008849/1‑139 (MQ=255) gTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCa > 2:965025/1‑133 (MQ=255) gTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCa < 1:965025/133‑1 (MQ=255) aaaGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGTACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCtttt < 1:1013831/139‑1 (MQ=255) aaaGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCtttt < 1:328097/139‑1 (MQ=255) aaaGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCAGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCtttt < 1:547520/139‑1 (MQ=255) aGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGGGGCGAGCACTCCTCGCAAGAGGAACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTCTTAt < 2:1008849/139‑1 (MQ=255) aGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTAt < 1:1308217/139‑1 (MQ=255) tcCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAAc > 2:1298399/1‑117 (MQ=255) tcCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAAc < 1:1298399/117‑1 (MQ=255) gcagcaAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTac > 2:333630/1‑60 (MQ=255) gcagcaAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTac < 1:333630/60‑1 (MQ=255) caAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTAc < 1:1461802/139‑1 (MQ=255) caAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTAc < 2:718029/139‑1 (MQ=255) aaTATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCgg < 1:480271/99‑1 (MQ=255) aaTATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCgg > 2:480271/1‑99 (MQ=255) tctTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATc > 1:1355647/1‑139 (MQ=255) ttGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTg > 2:272023/1‑103 (MQ=255) ttGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTg < 1:272023/103‑1 (MQ=255) tGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGc > 2:898009/1‑69 (MQ=255) tGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGc < 1:898009/69‑1 (MQ=255) tGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCg < 1:1413943/71‑1 (MQ=255) tGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCg > 2:1413943/1‑71 (MQ=255) tGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGAc > 2:417740/1‑73 (MQ=255) tGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGAc < 1:417740/73‑1 (MQ=255) tGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTg < 1:11300/102‑1 (MQ=255) tGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTg > 2:11300/1‑102 (MQ=255) gACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCa < 1:1462205/95‑1 (MQ=255) gACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCa > 2:1462205/1‑95 (MQ=255) gACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCaa > 2:1647197/1‑139 (MQ=255) aCGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTg > 2:432023/1‑63 (MQ=255) aCGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTg < 1:432023/63‑1 (MQ=255) aCGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCg < 1:1679868/74‑1 (MQ=255) aCGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCg > 2:1679868/1‑74 (MQ=255) cGAGCTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTACTCAGCGAAGCCGGGCTGGCGACCTACCGTACCCCGGAAGGAACCATCACTGGTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACt < 2:689216/139‑1 (MQ=255) cgAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCAt > 1:167089/1‑78 (MQ=255) cgAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCAt < 2:167089/78‑1 (MQ=255) cgAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACt > 2:1299374/1‑126 (MQ=255) cgAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACt < 1:1299374/126‑1 (MQ=255) gCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCg < 2:1355647/139‑1 (MQ=255) gCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCg < 1:1647197/139‑1 (MQ=255) cACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCggaa < 1:1191522/66‑1 (MQ=255) cACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCggaa > 2:1191522/1‑66 (MQ=255) ctcGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGccc > 2:1427888/1‑139 (MQ=255) ctcGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGccc < 1:191704/139‑1 (MQ=255) | GCCCAGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCC > NC_000913/2721068‑2721320 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |