Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,842,414	G→A	*475* (TAG→TAA)	ascB →	cryptic 6‑phospho‑beta‑glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,842,414	0	G	A	100.0%	208.1 / NA	64	*475* (TAG→TAA)	ascB	cryptic 6‑phospho‑beta‑glucosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/32);  total (32/32)

AATGAGCAAACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCC  >  NC_000913/2842282‑2842548                                                                                                                                     |                                                                                                                                       aaTGAGCAAACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACa                                                                                                                                  <  2:1536610/139‑1 (MQ=255)     aGCAAACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGc                                                                                                                              >  1:337098/1‑139 (MQ=255)       cAAACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCg                                                                                                                            >  1:1275790/1‑139 (MQ=255)                 ggCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGt                                                                                                                  >  2:841118/1‑139 (MQ=255)                            tgttgACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                       <  2:438941/139‑1 (MQ=255)                            tgttgACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                       <  2:337098/139‑1 (MQ=255)                            tgttgACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                       <  1:751855/139‑1 (MQ=255)                            tgttgACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                       <  1:681382/139‑1 (MQ=255)                                  ccGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                 <  1:1516214/139‑1 (MQ=255)                                      gacgacGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                       >  2:940634/1‑129 (MQ=255)                                      gacgacGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                       <  1:940634/129‑1 (MQ=255)                                        cgacgCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATg                                                                                           <  1:1042504/139‑1 (MQ=255)                                             cAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCa                                                                                      <  2:1275790/139‑1 (MQ=255)                                             cAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCa                                                                                      <  1:183707/139‑1 (MQ=255)                                                       tACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGa                                                                            >  1:44624/1‑139 (MQ=255)                                                          gCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAgg                                                                         >  1:1208686/1‑139 (MQ=255)                                                                      gcgTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGt                                                                                                                                <  1:1048085/72‑1 (MQ=255)                                                                      gcgTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGt                                                                                                                                >  2:1048085/1‑72 (MQ=255)                                                                                tCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTa                                                                                                        >  1:1612542/1‑86 (MQ=255)                                                                                tCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTa                                                                                                        <  2:1612542/86‑1 (MQ=255)                                                                                  aTTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCgg                                                   >  2:1122819/1‑137 (MQ=255)                                                                                  aTTCTGGTGCTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCgg                                                   <  1:1122819/137‑1 (MQ=255)                                                                                    tCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATaa                                                                 <  2:111343/121‑1 (MQ=255)                                                                                    tCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATaa                                                                 >  1:111343/1‑121 (MQ=255)                                                                                           gtATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCa                                                                                      <  2:1517162/93‑1 (MQ=255)                                                                                           gtATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCa                                                                                      >  1:1517162/1‑93 (MQ=255)                                                                                              taAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                       >  2:502425/1‑73 (MQ=255)                                                                                              taAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                       <  1:502425/73‑1 (MQ=255)                                                                                                   aaGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCAt                                <  2:1208686/139‑1 (MQ=255)                                                                                                      tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGAt                                                                   <  2:1505341/101‑1 (MQ=255)                                                                                                      tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGAt                                                                   >  1:1505341/1‑101 (MQ=255)                                                                                                      tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAgcgcagcgca                                                       <  2:1395760/113‑1 (MQ=255)                                                                                                      tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAgcgcagcgca                                                       >  1:1395760/1‑113 (MQ=255)                                                                                                      tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCag                             >  1:178567/1‑139 (MQ=255)                                                                                                      tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCag                             >  1:1702000/1‑139 (MQ=255)                                                                                                      tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCag                             >  1:1611415/1‑139 (MQ=255)                                                                                                      tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCag                             >  2:287038/1‑139 (MQ=255)                                                                                                       gATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCaga                            >  1:1204257/1‑139 (MQ=255)                                                                                                        aTTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCAt                                >  1:1269879/1‑134 (MQ=255)                                                                                                        aTTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCAt                                <  2:1269879/134‑1 (MQ=255)                                                                                                             cAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCgg                                                   <  1:44077/110‑1 (MQ=255)                                                                                                             cAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCgg                                                   >  2:44077/1‑110 (MQ=255)                                                                                                             cAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGt                      <  1:287038/139‑1 (MQ=255)                                                                                                                tAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCggg                                                  >  2:1034670/1‑108 (MQ=255)                                                                                                                tAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCggg                                                  <  1:1034670/108‑1 (MQ=255)                                                                                                                     gggAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCAttt                                     >  2:1166637/1‑116 (MQ=255)                                                                                                                     gggAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCAttt                                     <  1:1166637/116‑1 (MQ=255)                                                                                                                       gAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCAt                                >  2:1073759/1‑119 (MQ=255)                                                                                                                       gAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCAt                                <  1:1073759/119‑1 (MQ=255)                                                                                                                        aaGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCgg                                                   >  2:681693/1‑99 (MQ=255)                                                                                                                        aaGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCgg                                                   <  1:681693/99‑1 (MQ=255)                                                                                                                        aaGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCa           >  1:225223/1‑139 (MQ=255)                                                                                                                          gATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                       <  1:1321522/45‑1 (MQ=255)                                                                                                                          gATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                       >  2:1321522/1‑45 (MQ=255)                                                                                                                          gATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAg         >  2:435203/1‑139 (MQ=255)                                                                                                                          gATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACG‑ACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCa                                        >  1:1090971/1‑107 (MQ=255)                                                                                                                          gATTTAGACTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACG‑ACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCa                                        <  2:1090971/107‑1 (MQ=37)                                                                                                                            tttAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAt                                                                                            <  2:536193/54‑1 (MQ=255)                                                                                                                            tttAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAt                                                                                            >  1:536193/1‑54 (MQ=255)                                                                                                                            tttAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGaa       <  2:1611415/139‑1 (MQ=255)                                                                                                                             ttAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAAc      >  1:1076890/1‑139 (MQ=255)                                                                                                                              tAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACa     <  2:1630225/139‑1 (MQ=255)                                                                                                                                gagTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCAtt                                      >  2:392470/1‑104 (MQ=255)                                                                                                                                gagTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCAtt                                      <  1:392470/104‑1 (MQ=255)                                                                                                                                 agtaagtaACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATcc  <  2:1702000/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       AATGAGCAAACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCC  >  NC_000913/2842282‑2842548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑