Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,876,944 G→A A122T (GCC→ACC)  iap → aminopeptidase in alkaline phosphatase isozyme conversion

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,876,9440GA100.0% 234.3 / NA 73A122T (GCC→ACC) iapaminopeptidase in alkaline phosphatase isozyme conversion
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/36);  total (37/36)

AATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTA  >  NC_000913/2876812‑2877067
                                                                                                                                    |                                                                                                                           
aaTAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGc                                                                                                                       >  2:1434446/1‑139 (MQ=255)
               tataCCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACg                                                                                                        >  1:1322742/1‑139 (MQ=255)
                   ccgcccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGa                                                                                                    >  2:1627573/1‑139 (MQ=255)
                   ccgcccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGa                                                                                                    >  1:39341/1‑139 (MQ=255)
                      cccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGc                                                                                                 >  1:125298/1‑139 (MQ=255)
                      cccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGc                                                                                                 >  1:374597/1‑139 (MQ=255)
                              aaTCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTcg                                                                                         <  2:39341/139‑1 (MQ=255)
                                tCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTcggc                                                                                       <  2:1322742/139‑1 (MQ=255)
                                     agagCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCt                                                                                  <  2:374597/139‑1 (MQ=255)
                                       agCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGc                                                                                                                  >  2:257241/1‑105 (MQ=255)
                                       agCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGc                                                                                                                  <  1:257241/105‑1 (MQ=255)
                                            gCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACg                                                                              <  2:1468743/136‑1 (MQ=255)
                                            gCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACg                                                                              >  1:1468743/1‑136 (MQ=255)
                                              aTAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACa                                                                         <  1:1627573/139‑1 (MQ=255)
                                                      aCGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGTTGCTGACGCCGATGCCAATCTCGGCGGGc                                                                                   <  2:1001736/121‑1 (MQ=255)
                                                      aCGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGc                                                                                   >  1:1001736/1‑121 (MQ=255)
                                                      aCGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATgg                                                                 >  2:834950/1‑139 (MQ=255)
                                                            aGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCt                                                                                  >  1:1632203/1‑116 (MQ=255)
                                                            aGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCt                                                                                  <  2:1632203/116‑1 (MQ=255)
                                                              tACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTg                                                                                 >  1:1137926/1‑115 (MQ=255)
                                                              tACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTg                                                                                 <  2:1137926/115‑1 (MQ=255)
                                                                  gTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg                                                     <  1:1434446/139‑1 (MQ=255)
                                                                  gTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg                                                     <  1:834950/139‑1 (MQ=255)
                                                                   tGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccgc                                                    <  2:226207/139‑1 (MQ=255)
                                                                     aTTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACAcc                                                                                                                           >  2:798688/1‑66 (MQ=255)
                                                                     aTTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACAcc                                                                                                                           <  1:798688/66‑1 (MQ=255)
                                                                      ttGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAAt                                                                                             <  1:788192/95‑1 (MQ=255)
                                                                      ttGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAAt                                                                                             >  2:788192/1‑95 (MQ=255)
                                                                        gCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGtt                                               >  1:172929/1‑139 (MQ=255)
                                                                           gCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACAcc                                                                                                                           >  1:1424412/1‑60 (MQ=255)
                                                                           gCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACAcc                                                                                                                           <  2:1424412/60‑1 (MQ=255)
                                                                              cATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGgtg                                         <  1:756675/139‑1 (MQ=255)
                                                                               aTGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGgtgt                                        >  2:1213883/1‑139 (MQ=255)
                                                                               aTGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGgtgt                                        <  1:1213883/139‑1 (MQ=255)
                                                                                 gAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgcc                                                      >  1:1161391/1‑123 (MQ=255)
                                                                                 gAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgcc                                                      <  2:1161391/123‑1 (MQ=255)
                                                                                     gCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCt                                  <  2:172929/139‑1 (MQ=255)
                                                                                       aaaGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGgtg                                         >  2:73164/1‑130 (MQ=255)
                                                                                       aaaGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGgtg                                         <  1:73164/130‑1 (MQ=255)
                                                                                              cgcAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGgtg                                         <  2:717240/123‑1 (MQ=255)
                                                                                              cgcAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGgtg                                         >  1:717240/1‑123 (MQ=255)
                                                                                                     gATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGc                  <  2:125298/139‑1 (MQ=255)
                                                                                                                tGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATAc       >  2:1220132/1‑139 (MQ=255)
                                                                                                                tGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATAc       >  1:7042/1‑139 (MQ=255)
                                                                                                                 ggCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACg      >  2:35494/1‑139 (MQ=255)
                                                                                                                  gcgcATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg                                                     >  2:1067632/1‑91 (MQ=255)
                                                                                                                  gcgcATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg                                                     <  1:1067632/91‑1 (MQ=255)
                                                                                                                  gcgcATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGgtg                                         >  2:79074/1‑103 (MQ=255)
                                                                                                                  gcgcATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGgtg                                         <  1:79074/103‑1 (MQ=255)
                                                                                                                   cgcATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgcc                                                      <  2:1294176/89‑1 (MQ=255)
                                                                                                                   cgcATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgcc                                                      >  1:1294176/1‑89 (MQ=255)
                                                                                                                   cgcATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGcc    >  2:1563061/1‑139 (MQ=255)
                                                                                                                    gcATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAg                       >  1:1676824/1‑119 (MQ=255)
                                                                                                                    gcATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAg                       <  2:1676824/119‑1 (MQ=255)
                                                                                                                     cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCt                                                                                  <  2:1013116/59‑1 (MQ=255)
                                                                                                                     cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCt                                                                                  >  1:1013116/1‑59 (MQ=255)
                                                                                                                     cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACg                                                                              >  2:1336236/1‑63 (MQ=255)
                                                                                                                     cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACg                                                                              <  1:1336236/63‑1 (MQ=255)
                                                                                                                     cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGaa                              <  2:601409/111‑1 (MQ=255)
                                                                                                                     cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGaa                              >  1:601409/1‑111 (MQ=255)
                                                                                                                     cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGa                               <  2:890517/110‑1 (MQ=255)
                                                                                                                     cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGa                               >  1:890517/1‑110 (MQ=255)
                                                                                                                     cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGc                  >  2:504220/1‑123 (MQ=255)
                                                                                                                     cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGc                  <  1:504220/123‑1 (MQ=255)
                                                                                                                     cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTa  <  1:1220132/139‑1 (MQ=255)
                                                                                                                       tCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTg                                                                                 >  2:466855/1‑58 (MQ=255)
                                                                                                                       tCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTg                                                                                 <  1:466855/58‑1 (MQ=255)
                                                                                                                        cTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgcc                                                      >  2:1002413/1‑84 (MQ=255)
                                                                                                                        cTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgcc                                                      <  1:1002413/84‑1 (MQ=255)
                                                                                                                                ttACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACg                                                                              <  2:1194612/52‑1 (MQ=255)
                                                                                                                                ttACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACg                                                                              >  1:1194612/1‑52 (MQ=255)
                                                                                                                                ttACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGa                                                                     >  2:562053/1‑61 (MQ=255)
                                                                                                                                ttACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGa                                                                     <  1:562053/61‑1 (MQ=255)
                                                                                                                                    |                                                                                                                           
AATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTA  >  NC_000913/2876812‑2877067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑