Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 280,024 C→T pseudogene (94/174 nt) insI1 → IS30 transposase,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913280,0240CT100.0% 110.0 / NA 39pseudogene (94/174 nt)insI1IS30 transposase,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/18);  total (21/18)

AAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTAACCCGTTTCAGC  >  NC_000913/279889‑280156
                                                                                                                                       |                                                                                                                                    
aaaCACCATCATACACTAAATCAGCAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTaa                                                                                                                                   >  2:1676696/1‑139 (MQ=255)
        tcatACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGa                                                                                                                           <  2:71398/139‑1 (MQ=255)
                  aaTCAGTAATTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGa                                                                                                                 <  2:1022829/139‑1 (MQ=255)
                               gcagcaTCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCaa                                                                                                    <  2:140247/139‑1 (MQ=255)
                               gcagcaTCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCaa                                                                                                    <  1:1676696/139‑1 (MQ=255)
                                  gcaTCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCaa                                                                                                    >  2:785828/1‑136 (MQ=37)
                                  gcaTCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCaa                                                                                                    <  1:785828/136‑1 (MQ=37)
                                   caTCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAaca                                                                                                <  2:911967/139‑1 (MQ=25)
                                     tCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACAcc                                                                                              >  1:733366/1‑139 (MQ=18)
                                      cACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCg                                                                                             >  2:768983/1‑139 (MQ=18)
                                        ccGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGaa                                                                                           <  2:1065698/139‑1 (MQ=14)
                                        ccGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGaa                                                                                           <  1:768983/139‑1 (MQ=14)
                                                                                      aTGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTAcagc                                             >  1:1008110/1‑139 (MQ=11)
                                                                                      aTGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTAcagc                                             >  2:363707/1‑139 (MQ=11)
                                                                                      aTGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTAcagc                                             >  1:659267/1‑139 (MQ=11)
                                                                                         tCTTGCCCAATACACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAAAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACagcagc                                          <  2:792036/139‑1 (MQ=12)
                                                                                              cccAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCttttt                                     >  2:1538773/1‑139 (MQ=21)
                                                                                              cccAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCttttt                                     <  2:1322803/139‑1 (MQ=37)
                                                                                                   tataCTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAat                                <  2:1008110/139‑1 (MQ=255)
                                                                                                   tataCTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAat                                >  2:1189469/1‑139 (MQ=255)
                                                                                                        tCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTc                           >  1:1573493/1‑139 (MQ=255)
                                                                                                        tCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTc                           >  2:110512/1‑139 (MQ=255)
                                                                                                        tCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTc                           >  2:1440624/1‑139 (MQ=255)
                                                                                                            cATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGt                       >  2:634382/1‑139 (MQ=255)
                                                                                                            cATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGt                       >  1:391383/1‑139 (MQ=255)
                                                                                                             aTGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCtt                                        >  1:104485/1‑121 (MQ=25)
                                                                                                             aTGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCtt                                        <  2:104485/121‑1 (MQ=25)
                                                                                                             aTGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGtt                      >  2:1304123/1‑139 (MQ=255)
                                                                                                                 aCTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTAcagcag                                           >  2:1305042/1‑114 (MQ=17)
                                                                                                                 aCTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTAcagcag                                           <  1:1305042/114‑1 (MQ=17)
                                                                                                                 aCTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTcc                          >  1:1317966/1‑131 (MQ=255)
                                                                                                                 aCTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTtcgtc                  >  1:945478/1‑139 (MQ=255)
                                                                                                                 aCTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGGTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTcc                          <  2:1317966/131‑1 (MQ=255)
                                                                                                                      aTCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTaa             <  2:945478/139‑1 (MQ=255)
                                                                                                                           gTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGCTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTAACCCGt        <  1:1304123/139‑1 (MQ=255)
                                                                                                                               ctgctCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTAAcc           >  2:439708/1‑132 (MQ=255)
                                                                                                                               ctgctCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTAAcc           <  1:439708/132‑1 (MQ=255)
                                                                                                                               ctgctCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTAACCCGTTTCa    >  1:733133/1‑139 (MQ=255)
                                                                                                                                 gctCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTAACCCGTTTCAGc  <  1:270701/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                    
AAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTAACCCGTTTCAGC  >  NC_000913/279889‑280156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: