Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,970,351	G→A	*230* (TAG→TAA)	mutH →	methyl‑directed mismatch repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,970,351	0	G	A	100.0%	146.0 / NA	45	*230* (TAG→TAA)	mutH	methyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/23);  total (22/23)

CGAAAGCAGCGAATGCGAAAGCGCTTACCGAAGCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACG  >  NC_000913/2970215‑2970476                                                                                                                                         |                                                                                                                              cGAAAGCAGCGAATGCGAAAGCGCTTACCGAAGCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAAcc                                                                                                                             <  1:974157/139‑1 (MQ=255)    aaGCAGCGAATGCGAAAGCGCTTACCGAAGCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATc                                                                                                                          >  2:107698/1‑139 (MQ=255)                                     ttGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTa                                                                                         >  2:953486/1‑139 (MQ=255)                                      tGGTGCCCGGGGCGAACGGATTCTGACACTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTAc                                                                                        >  2:1231052/1‑139 (MQ=255)                                            ccGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTc                                                                                  <  1:953486/139‑1 (MQ=255)                                                ggCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCtt                                                                                                                      >  2:446071/1‑99 (MQ=255)                                                ggCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCtt                                                                                                                      <  1:446071/99‑1 (MQ=255)                                                  cGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGAc                                                                                                                  >  2:1659137/1‑101 (MQ=255)                                                  cGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGAc                                                                                                                  <  1:1659137/101‑1 (MQ=255)                                                    aaCGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCAttttttt                                                                          >  2:981506/1‑139 (MQ=255)                                                        gATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCa                                                                      <  1:981506/139‑1 (MQ=255)                                                           tCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAggg                                                                   <  1:107698/139‑1 (MQ=255)                                                                                   tttGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   <  1:165995/83‑1 (MQ=255)                                                                                   tttGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   >  2:165995/1‑83 (MQ=255)                                                                                                    ccAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGcc                          >  2:622881/1‑139 (MQ=255)                                                                                                        tGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATg                                             >  1:132382/1‑116 (MQ=255)                                                                                                        tGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATg                                             <  2:132382/116‑1 (MQ=255)                                                                                                        tGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGc                      <  1:622881/139‑1 (MQ=255)                                                                                                        tGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGc                      >  2:642233/1‑139 (MQ=255)                                                                                                               ctGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCt               <  1:642233/139‑1 (MQ=255)                                                                                                                 ggCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCg             <  1:1456339/139‑1 (MQ=255)                                                                                                                  gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGAc                                                                                                                  >  1:654326/1‑37 (MQ=255)                                                                                                                  gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGAc                                                                                                                  <  2:654326/37‑1 (MQ=255)                                                                                                                  gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTgg                                                                        <  2:459649/79‑1 (MQ=255)                                                                                                                  gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTgg                                                                        >  1:459649/1‑79 (MQ=255)                                                                                                                  gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTAttt                                                  <  2:1259265/101‑1 (MQ=255)                                                                                                                  gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTAttt                                                  >  1:1259265/1‑101 (MQ=255)                                                                                                                  gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGc                                                <  1:32772/103‑1 (MQ=255)                                                                                                                  gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGc                                                >  2:32772/1‑103 (MQ=255)                                                                                                                    ccGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGc                                                <  2:1183351/101‑1 (MQ=255)                                                                                                                    ccGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGc                                                >  1:1183351/1‑101 (MQ=255)                                                                                                                       tCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   <  2:1016928/47‑1 (MQ=255)                                                                                                                       tCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   >  1:1016928/1‑47 (MQ=255)                                                                                                                       tCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAggg                                                                   >  2:1053809/1‑79 (MQ=255)                                                                                                                       tCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTa                                                            >  1:772369/1‑86 (MQ=255)                                                                                                                       tCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTa                                                            <  2:772369/86‑1 (MQ=255)                                                                                                                       tCATTTGCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAggg                                                                   <  1:1053809/79‑1 (MQ=255)                                                                                                                                 aTCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAg                                                           >  2:1575251/1‑77 (MQ=255)                                                                                                                                 aTCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAg                                                           <  1:1575251/77‑1 (MQ=255)                                                                                                                                 aTCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTg                                                 >  2:1173621/1‑87 (MQ=255)                                                                                                                                 aTCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTg                                                 <  1:1173621/87‑1 (MQ=255)                                                                                                                                 aTCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCGGTacgctgacg  <  2:278478/134‑1 (MQ=255)                                                                                                                                 aTCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCGGTacgctgacg  >  1:278478/1‑134 (MQ=255)                                                                                                                                  tCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCg                                                                                      >  1:861146/1‑49 (MQ=255)                                                                                                                                  tCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCg                                                                                      <  2:861146/49‑1 (MQ=255)                                                                                                                                         |                                                                                                                              CGAAAGCAGCGAATGCGAAAGCGCTTACCGAAGCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACG  >  NC_000913/2970215‑2970476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑