Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	287,977	A→G	M397V (ATG→GTG)	yagH →	CP4‑6 prophage, putative xylosidase/arabinosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	287,977	0	A	G	100.0%	184.5 / NA	50	M397V (ATG→GTG)	yagH	CP4‑6 prophage, putative xylosidase/arabinosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/31);  total (19/31)

GCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACA  >  NC_000913/287855‑288112                                                                                                                           |                                                                                                                                        gctcgctcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGt                                                                                                                         <  2:557410/139‑1 (MQ=255) gctcgctcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGt                                                                                                                         <  2:379797/139‑1 (MQ=255)      ctcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACt                                                                                                                    >  2:1336710/1‑139 (MQ=255)               cgcCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCaga                                                                                                           >  2:930946/1‑139 (MQ=255)                  ccGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAgcg                                                                                                        <  1:755427/139‑1 (MQ=255)                       cttcttACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCgggg                                                                                                   <  1:537653/139‑1 (MQ=255)                        ttcttACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGc                                                                                                  <  1:581912/139‑1 (MQ=255)                           ttACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCgggg                                                                                                   <  2:1432082/135‑1 (MQ=255)                           ttACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCgggg                                                                                                   >  1:1432082/1‑135 (MQ=255)                           ttACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGa                                                                                               <  1:916208/139‑1 (MQ=255)                           ttACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGa                                                                                               <  1:715394/139‑1 (MQ=255)                            tACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGAc                                                                                              >  1:87535/1‑139 (MQ=255)                            tACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGAc                                                                                              <  1:1514201/139‑1 (MQ=255)                              cGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCt                                                                                            <  2:87535/139‑1 (MQ=255)                               ggCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTg                                                                                           >  2:627152/1‑139 (MQ=255)                                                  gCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAAc                                                                        >  2:78052/1‑139 (MQ=255)                                                        ttCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGc                                                                  >  2:1653825/1‑139 (MQ=255)                                                        ttCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGc                                                                  <  1:1653825/139‑1 (MQ=255)                                                        ttCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGc                                                                  <  1:810901/139‑1 (MQ=255)                                                               ttCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCt                                                           <  1:78052/139‑1 (MQ=255)                                                                  aCCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTtg                                                        <  2:1331545/139‑1 (MQ=255)                                                                       aTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCt                                                                                                 <  1:1314795/93‑1 (MQ=255)                                                                       aTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCt                                                                                                 >  2:1314795/1‑93 (MQ=255)                                                                         cGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTa                                                 <  1:930946/139‑1 (MQ=255)                                                                           aCCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCt                                                                                            <  2:479377/94‑1 (MQ=255)                                                                           aCCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCt                                                                                            >  2:1502026/1‑94 (MQ=255)                                                                           aCCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCt                                                                                            <  1:131073/94‑1 (MQ=255)                                                                           aCCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCt                                                                                            <  1:1502026/94‑1 (MQ=255)                                                                           aCCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCt                                                                                            >  1:479377/1‑94 (MQ=255)                                                                           aCCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCt                                                                                            >  2:131073/1‑94 (MQ=255)                                                                           aCCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTa                                                                <  2:682416/122‑1 (MQ=255)                                                                           aCCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTa                                                                >  1:682416/1‑122 (MQ=255)                                                                           aCCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACg                                               >  1:510699/1‑139 (MQ=255)                                                                              gTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACgagg                                            >  2:462366/1‑139 (MQ=255)                                                                              gTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACgagg                                            <  1:462366/139‑1 (MQ=255)                                                                              gTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACgagg                                            <  1:310633/139‑1 (MQ=255)                                                                              gTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACgagg                                            <  1:1336710/139‑1 (MQ=255)                                                                                  cgcgcCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCcagca                                                                                                             <  1:805772/70‑1 (MQ=255)                                                                                  cgcgcCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCcagca                                                                                                             >  2:805772/1‑70 (MQ=255)                                                                                   gcgcCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGa                                       <  1:627152/139‑1 (MQ=255)                                                                                     gccgcTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACa                                     <  2:510699/139‑1 (MQ=255)                                                                                                   ttCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGAc                                                                                              >  2:410897/1‑68 (MQ=255)                                                                                                   ttCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGAc                                                                                              <  1:1357429/68‑1 (MQ=255)                                                                                                   ttCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGAc                                                                                              <  1:410897/68‑1 (MQ=255)                                                                                                   ttCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGAc                                                                                              >  2:1357429/1‑68 (MQ=255)                                                                                                      gCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCa                                                                             >  1:1273477/1‑82 (MQ=255)                                                                                                      gCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCa                                                                             <  2:1273477/82‑1 (MQ=255)                                                                                                                     aCGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCt                                                                                            <  1:899786/52‑1 (MQ=255)                                                                                                                     aCGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCt                                                                                            >  2:899786/1‑52 (MQ=255)                                                                                                                        cgGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACcaca  <  2:1723344/139‑1 (MQ=255)                                                                                                                           |                                                                                                                                        GCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACA  >  NC_000913/287855‑288112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑