Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,056,273 G→A P11P (CCG→CCA)  fau → 5‑formyltetrahydrofolate cyclo‑ligase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,056,2730GA100.0% 279.6 / NA 84P11P (CCG→CCA) fau5‑formyltetrahydrofolate cyclo‑ligase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/43);  total (41/43)

ACAGCCTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTC  >  NC_000913/3056138‑3056396
                                                                                                                                       |                                                                                                                           
aCAGCCTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaa                                                                                                                          >  2:1269798/1‑139 (MQ=255)
 cAGCCTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaac                                                                                                                         <  2:964620/139‑1 (MQ=255)
      tGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTCTCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAAcag                                                                                                                    <  2:1129086/139‑1 (MQ=255)
      tGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAAcag                                                                                                                    >  1:474398/1‑139 (MQ=255)
                          tCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGc                                                                                                <  1:1327168/139‑1 (MQ=255)
                                 ttATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTAcc                                                                                         <  2:1538692/139‑1 (MQ=255)
                                        gCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTAcc                                                                                         >  1:931174/1‑132 (MQ=255)
                                        gCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTAcc                                                                                         <  2:931174/132‑1 (MQ=255)
                                        gCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatga                                                                                  >  2:43426/1‑139 (MQ=255)
                                        gCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatga                                                                                  >  1:1598787/1‑139 (MQ=255)
                                         cACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatgat                                                                                 <  1:1628670/139‑1 (MQ=255)
                                             aGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatga                                                                                  >  1:1069817/1‑134 (MQ=255)
                                             aGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatga                                                                                  <  2:1069817/134‑1 (MQ=255)
                                              gCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACtt                                                                            >  1:882386/1‑139 (MQ=255)
                                                  tGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaacaa                                                                                                                       <  1:1170569/92‑1 (MQ=255)
                                                  tGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaacaa                                                                                                                       >  2:1170569/1‑92 (MQ=255)
                                                  tGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATcc                                                                        >  1:411888/1‑139 (MQ=255)
                                                         aCTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAATGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCggtg                                                                 >  1:1386185/1‑139 (MQ=255)
                                                         aCTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAAcag                                                                                                                    <  2:278215/88‑1 (MQ=255)
                                                         aCTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAAcag                                                                                                                    >  1:278215/1‑88 (MQ=255)
                                                         aCTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatgat                                                                                 >  1:1554524/1‑123 (MQ=255)
                                                         aCTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatgat                                                                                 <  2:1554524/123‑1 (MQ=255)
                                                         aCTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCggtg                                                                 >  2:1440109/1‑139 (MQ=255)
                                                           tACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCggtggt                                                               <  2:1621022/139‑1 (MQ=255)
                                                           tACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCggtggt                                                               <  2:1141589/139‑1 (MQ=255)
                                                            aCCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                              <  2:1598787/139‑1 (MQ=255)
                                                              cAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGAt                                                            <  1:1269798/139‑1 (MQ=255)
                                                               aGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAATGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATg                                                           <  2:1386185/139‑1 (MQ=255)
                                                                gAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATgg                                                          <  2:1688447/139‑1 (MQ=255)
                                                                    tCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                              <  2:104966/131‑1 (MQ=255)
                                                                    tCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                              >  1:104966/1‑131 (MQ=255)
                                                                     cccACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACAt                                                     >  1:580270/1‑139 (MQ=255)
                                                                      ccACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaacaa                                                                                                                       >  1:981750/1‑72 (MQ=255)
                                                                      ccACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaacaa                                                                                                                       <  2:981750/72‑1 (MQ=255)
                                                                      ccACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATa                                                    >  1:898519/1‑139 (MQ=255)
                                                                       cACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAg                                                                                                 >  1:1467825/1‑93 (MQ=255)
                                                                       cACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAg                                                                                                 <  2:1467825/93‑1 (MQ=255)
                                                                       cACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                              <  2:179936/128‑1 (MQ=255)
                                                                       cACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                              >  1:179936/1‑128 (MQ=255)
                                                                         cTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACgg                                                 <  2:580270/139‑1 (MQ=255)
                                                                          tGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGt                                                <  2:411888/139‑1 (MQ=255)
                                                                           gACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTc                                               >  2:506658/1‑139 (MQ=255)
                                                                            aCATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGc                                                                                                <  1:45908/89‑1 (MQ=255)
                                                                            aCATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGc                                                                                                >  2:45908/1‑89 (MQ=255)
                                                                            aCATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCg                                              >  1:1329068/1‑139 (MQ=255)
                                                                                 aTCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTa                                         <  2:882386/139‑1 (MQ=255)
                                                                                      gACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCct                                    >  1:566881/1‑139 (MQ=255)
                                                                                       aCAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTcaac                                                                                                    <  2:586538/74‑1 (MQ=255)
                                                                                       aCAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTcaac                                                                                                    >  1:586538/1‑74 (MQ=255)
                                                                                       aCAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGc                                                                                                <  2:1056833/78‑1 (MQ=255)
                                                                                       aCAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGc                                                                                                >  1:480361/1‑78 (MQ=255)
                                                                                       aCAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGc                                                                                                <  2:480361/78‑1 (MQ=255)
                                                                                       aCAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGc                                                                                                >  1:1056833/1‑78 (MQ=255)
                                                                                       aCAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCggtggt                                                               <  1:1385093/111‑1 (MQ=255)
                                                                                       aCAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCggtggt                                                               >  2:1385093/1‑111 (MQ=255)
                                                                                       aCAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACgg                                                 <  1:780030/125‑1 (MQ=255)
                                                                                       aCAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACgg                                                 >  2:780030/1‑125 (MQ=255)
                                                                                        cAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCctct                                  >  1:272951/1‑139 (MQ=255)
                                                                                        cAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCctct                                  >  2:1105038/1‑139 (MQ=255)
                                                                                          ggAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                              >  1:146522/2‑109 (MQ=255)
                                                                                          ggAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                              <  2:146522/108‑1 (MQ=255)
                                                                                           gAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCtt                               >  1:568304/1‑139 (MQ=255)
                                                                                                   cAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGc                       <  2:566881/139‑1 (MQ=255)
                                                                                                      aaTGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGaa                                                                                                              <  1:1393903/49‑1 (MQ=255)
                                                                                                      aaTGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGaa                                                                                                              >  2:1393903/1‑49 (MQ=255)
                                                                                                               gCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACAcc           <  2:898519/139‑1 (MQ=255)
                                                                                                                cAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                              <  1:52151/87‑1 (MQ=255)
                                                                                                                cAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                              >  2:52151/1‑87 (MQ=255)
                                                                                                                cAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATa                                                    >  2:1563701/1‑97 (MQ=255)
                                                                                                                cAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATa                                                    <  1:1563701/97‑1 (MQ=255)
                                                                                                                cAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACgg                                                 >  1:1017819/1‑100 (MQ=255)
                                                                                                                cAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACgg                                                 <  2:1017819/100‑1 (MQ=255)
                                                                                                                    gtcgtcgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTAcc                                                                                         >  2:533987/1‑56 (MQ=255)
                                                                                                                    gtcgtcgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTAcc                                                                                         <  1:533987/56‑1 (MQ=255)
                                                                                                                    gtcgtcgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGcc      <  1:43426/139‑1 (MQ=255)
                                                                                                                    gtcgtcgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGcc      <  2:272951/139‑1 (MQ=255)
                                                                                                                        tcgtGCGTTAACGCCAGAACAACAGCGGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTc  >  2:519145/1‑139 (MQ=255)
                                                                                                                        tcgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTcaac                                                                                                    <  2:1159373/41‑1 (MQ=255)
                                                                                                                        tcgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTaaac                                                                                                    >  1:1159373/1‑41 (MQ=25)
                                                                                                                        tcgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTc  <  1:1440109/139‑1 (MQ=255)
                                                                                                                               ttAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                              <  2:717819/72‑1 (MQ=255)
                                                                                                                               ttAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                              >  1:717819/1‑72 (MQ=255)
                                                                                                                               ttAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTAtt                                       <  2:700989/95‑1 (MQ=255)
                                                                                                                               ttAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTAtt                                       >  1:700989/1‑95 (MQ=255)
                                                                                                                                       |                                                                                                                           
ACAGCCTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTC  >  NC_000913/3056138‑3056396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑