Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,085,461 A→G S151P (TCC→CCC)  speA ← biosynthetic arginine decarboxylase, PLP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,085,4610AG100.0% 220.8 / NA 62S151P (TCC→CCC) speAbiosynthetic arginine decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/33);  total (29/33)

CCAGATAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACG  >  NC_000913/3085326‑3085595
                                                                                                                                       |                                                                                                                                      
ccAGATAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGAcc                                                                                                                                     <  2:473401/139‑1 (MQ=255)
  aGATAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCgg                                                                                                                                   >  1:1647709/1‑139 (MQ=255)
     tAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCtt                                                                                                                                >  1:1706802/1‑139 (MQ=255)
       gACCTTGTTCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTcc                                                                                                                              <  1:1031416/139‑1 (MQ=255)
       gACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTcc                                                                                                                              <  1:723592/139‑1 (MQ=255)
        aCCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCa                                                                                                                             >  2:1178279/1‑139 (MQ=255)
             gtgCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGAccc                                                                                                                        >  2:923642/1‑139 (MQ=255)
             gtgCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGAccc                                                                                                                        <  2:1647709/139‑1 (MQ=255)
                  ccATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCgg                                                                                                                   >  2:1687310/1‑139 (MQ=255)
                   cATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGt                                                                                                                  <  1:1383135/139‑1 (MQ=255)
                   cATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGt                                                                                                                  >  1:703718/1‑139 (MQ=255)
                     tcttctCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCgg                                                                                                                   <  2:1228436/136‑1 (MQ=255)
                     tcttctCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCgg                                                                                                                   >  1:1228436/1‑136 (MQ=255)
                       ttctCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGc                                                                                                              <  1:846037/139‑1 (MQ=255)
                        tctcGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGcc                                                                                                             <  1:1025924/139‑1 (MQ=255)
                             ccAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCgaat                                                                                                        >  2:1087983/1‑139 (MQ=255)
                                 ttAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCgaatgaat                                                                                                    >  1:159761/1‑139 (MQ=255)
                                     tGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGAcc                                                                                                                                     >  1:314345/1‑102 (MQ=255)
                                     tGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGAcc                                                                                                                                     <  2:314345/102‑1 (MQ=255)
                                               atatatTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCa                                                                                      <  2:1441892/139‑1 (MQ=255)
                                                    ttCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACgcgg                                                                                 <  1:179487/139‑1 (MQ=255)
                                                       gcgGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGcggcgg                                                                              <  1:1087983/139‑1 (MQ=255)
                                                         ggTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACgcgg                                                                                 >  1:361562/1‑134 (MQ=255)
                                                         ggTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACgcgg                                                                                 <  2:361562/134‑1 (MQ=255)
                                                         ggTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                            <  2:159761/139‑1 (MQ=255)
                                                         ggTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                            >  2:830104/1‑139 (MQ=255)
                                                         ggTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                            >  1:41358/1‑139 (MQ=255)
                                                            cTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTg                                                                         >  1:1335314/1‑139 (MQ=255)
                                                              ttATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGt                                                                       <  1:830104/139‑1 (MQ=255)
                                                                        tGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCTCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGAt                                                             <  2:903367/139‑1 (MQ=255)
                                                                         gCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGa                                                              >  2:344731/1‑137 (MQ=255)
                                                                         gCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGa                                                              <  1:344731/137‑1 (MQ=255)
                                                                             aCGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGAccc                                                                                                                        >  2:1389797/1‑75 (MQ=255)
                                                                             aCGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGAccc                                                                                                                        <  1:1389797/75‑1 (MQ=255)
                                                                                  gACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACgcgg                                                                                 >  2:1164968/1‑109 (MQ=255)
                                                                                  gACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACgcgg                                                                                 <  1:1164968/109‑1 (MQ=255)
                                                                                           ggTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCa                                                                                                  <  1:1284184/83‑1 (MQ=255)
                                                                                           ggTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCa                                                                                                  >  2:1284184/1‑83 (MQ=255)
                                                                                             tCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATc                                        <  2:1335314/139‑1 (MQ=255)
                                                                                             tCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATc                                        <  1:936094/139‑1 (MQ=255)
                                                                                               aTGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATaaa                                                     <  1:372180/124‑1 (MQ=255)
                                                                                               aTGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATaaa                                                     >  2:372180/1‑124 (MQ=255)
                                                                                                tGCCAGCATGTGCCAGTACCGCCCTCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGAATCAATTACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGcc                                     >  2:910694/1‑139 (MQ=255)
                                                                                                    aGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCTGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTta                                 <  1:923642/139‑1 (MQ=255)
                                                                                                    aGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTta                                 <  1:1687310/139‑1 (MQ=255)
                                                                                                    aGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTta                                 <  2:7286/139‑1 (MQ=255)
                                                                                                    aGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTta                                 <  2:41358/139‑1 (MQ=255)
                                                                                                           gCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTg                                                               <  2:1625586/102‑1 (MQ=255)
                                                                                                           gCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTg                                                               >  1:1625586/1‑102 (MQ=255)
                                                                                                                    gCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTccc                 >  1:432703/1‑139 (MQ=255)
                                                                                                                     ccATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCg                                       >  1:1441879/1‑116 (MQ=255)
                                                                                                                     ccATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCg                                       <  2:1441879/116‑1 (MQ=255)
                                                                                                                     ccATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCt                >  1:533939/1‑139 (MQ=255)
                                                                                                                     ccATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCt                >  2:1489903/1‑139 (MQ=255)
                                                                                                                        tCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCg                                                                                                                    >  2:668707/1‑36 (MQ=255)
                                                                                                                        tCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCg                                                                                                                    <  1:668707/36‑1 (MQ=255)
                                                                                                                         cAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCa            >  2:575450/1‑139 (MQ=255)
                                                                                                                             tCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGTGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTc                   <  1:1069708/128‑1 (MQ=255)
                                                                                                                             tCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGAACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTc                   >  2:1069708/1‑128 (MQ=255)
                                                                                                                               ggCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTg      >  2:1618115/1‑139 (MQ=255)
                                                                                                                                  tttGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAAc   <  2:1126188/139‑1 (MQ=255)
                                                                                                                                   ttGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAAcg  <  1:575450/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CCAGATAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACG  >  NC_000913/3085326‑3085595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑