Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,176,721 G→A T38I (ACC→ATC)  cpdA ← 3',5' cAMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,176,7210GA100.0% 270.5 / NA 82T38I (ACC→ATC) cpdA3',5' cAMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/43);  total (39/43)

TTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCAC  >  NC_000913/3176587‑3176853
                                                                                                                                      |                                                                                                                                    
ttGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGtt                                                                                                                                  <  1:1376166/139‑1 (MQ=255)
  gCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTa                                                                                                                                >  2:968201/1‑139 (MQ=255)
   cGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTAc                                                                                                                               >  1:956637/1‑139 (MQ=255)
                     gCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTg                                                                                                             <  2:1401630/139‑1 (MQ=255)
                         aTAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCttt                                                                                                         >  2:1277064/1‑139 (MQ=255)
                            ggCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTtgt                                                                                                      <  1:667134/139‑1 (MQ=255)
                            ggCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTtgt                                                                                                      <  2:1348183/139‑1 (MQ=255)
                                 cAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCaaa                                                                                                 >  1:1329074/1‑139 (MQ=255)
                                   gagGATTGATCCTGCGCTAACTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTAcc                                                                                                                              >  2:1018538/1‑108 (MQ=255)
                                   gagGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTAcc                                                                                                                              <  1:1018538/108‑1 (MQ=255)
                                      gATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGt                                                                                            <  1:1277064/139‑1 (MQ=255)
                                      gATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGt                                                                                            <  2:1329074/139‑1 (MQ=255)
                                         tGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAg                                                                                         <  1:569826/139‑1 (MQ=255)
                                              cTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCg                                                                                    >  2:1447464/1‑139 (MQ=255)
                                                gcgcTAATTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAg                                                                                                                      >  2:1134461/1‑103 (MQ=255)
                                                gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAg                                                                                                                      <  1:1134461/103‑1 (MQ=255)
                                                gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGt                                                                                            >  2:557741/1‑129 (MQ=255)
                                                gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGt                                                                                            <  1:557741/129‑1 (MQ=255)
                                                gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGt                                                                                  >  2:724272/1‑139 (MQ=255)
                                                gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGt                                                                                  >  1:855394/1‑139 (MQ=255)
                                                 cgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTa                                                                                 >  2:430642/1‑139 (MQ=255)
                                                      aaTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCt                                                                                                                  >  1:1012877/1‑101 (MQ=255)
                                                      aaTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGt                                                                                            <  1:985867/123‑1 (MQ=255)
                                                      aaTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGt                                                                                            >  2:985867/1‑123 (MQ=255)
                                                      aaTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTg                                                                                           >  2:529652/1‑124 (MQ=255)
                                                      aaTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTg                                                                                           <  1:529652/124‑1 (MQ=255)
                                                      aaTCACCTGTCGCGACAATCAGGTCGAATTCGTCCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCt                                                                                                                  <  2:1012877/101‑1 (MQ=255)
                                                       aTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGt                                                                           >  1:1669298/1‑139 (MQ=255)
                                                        tCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTa                                                                          <  1:135106/139‑1 (MQ=255)
                                                           ccTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGatgt                                                                                                                                   <  1:285825/79‑1 (MQ=255)
                                                           ccTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGatgt                                                                                                                                   >  2:285825/1‑79 (MQ=255)
                                                           ccTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGtt                                                                                                                                  <  2:736064/80‑1 (MQ=255)
                                                           ccTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGtt                                                                                                                                  >  1:736064/1‑80 (MQ=255)
                                                           ccTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCa                                                                                                   <  2:1112130/111‑1 (MQ=255)
                                                           ccTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCa                                                                                                   >  1:1112130/1‑111 (MQ=255)
                                                           ccTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCg                                                                                    >  2:1671419/1‑126 (MQ=255)
                                                           ccTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCg                                                                                    <  1:1671419/126‑1 (MQ=255)
                                                           ccTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTaaaa                                                                       >  1:73044/1‑139 (MQ=255)
                                                              gTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATcc                                                                    <  1:1447464/139‑1 (MQ=255)
                                                                cgcgACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTg                                                                  >  2:302694/1‑139 (MQ=255)
                                                                cgcgACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTg                                                                  >  1:1275842/1‑139 (MQ=255)
                                                                cgcgACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTg                                                                  >  1:99428/1‑139 (MQ=255)
                                                                  cgACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTAcc                                                                                                                              <  1:1301002/77‑1 (MQ=255)
                                                                  cgACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTAcc                                                                                                                              >  2:1301002/1‑77 (MQ=255)
                                                                  cgACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGAc                                                                >  1:1465238/1‑139 (MQ=255)
                                                                   gACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGAct                                                               <  1:302694/139‑1 (MQ=255)
                                                                    aCAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGatgt                                                                                                                                   <  2:1117339/70‑1 (MQ=255)
                                                                    aCAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGatgt                                                                                                                                   >  1:1117339/1‑70 (MQ=255)
                                                                      aaTCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCa                                                                                                   >  2:292425/1‑100 (MQ=255)
                                                                      aaTCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCa                                                                                                   <  1:292425/100‑1 (MQ=255)
                                                                       aTCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTgg                                                           <  1:430642/139‑1 (MQ=255)
                                                                       aTCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTgg                                                           <  1:1268133/139‑1 (MQ=255)
                                                                         cAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGcc                                                         <  2:327728/139‑1 (MQ=255)
                                                                           ggTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCa                                                                                                   >  1:714319/1‑95 (MQ=255)
                                                                           ggTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCa                                                                                                   <  2:714319/95‑1 (MQ=255)
                                                                            gTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCa                                                      <  2:1669298/139‑1 (MQ=255)
                                                                              cGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAcc                                                    <  1:1695390/139‑1 (MQ=255)
                                                                                 aTTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAccagc                                                 <  2:734083/139‑1 (MQ=255)
                                                                                 aTTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAccagc                                                 >  1:1604951/1‑139 (MQ=255)
                                                                                  ttCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAccagcc                                                >  1:1652778/1‑139 (MQ=255)
                                                                                  ttCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAc                                                     <  2:1078573/134‑1 (MQ=255)
                                                                                  ttCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAc                                                     >  1:1078573/1‑134 (MQ=255)
                                                                                    cGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAccagccag                                              >  1:859806/1‑139 (MQ=255)
                                                                                               ggCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTa                                   <  2:73044/139‑1 (MQ=255)
                                                                                                gCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTaa                                  >  2:1341051/1‑139 (MQ=255)
                                                                                                    aaTCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAgg                              <  2:1604951/139‑1 (MQ=255)
                                                                                                     aTCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCt                                                                                                           >  1:403649/1‑61 (MQ=255)
                                                                                                     aTCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCt                                                                                                           <  2:403649/61‑1 (MQ=255)
                                                                                                     aTCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGc                             <  1:968201/139‑1 (MQ=255)
                                                                                                     aTCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGc                             <  1:724272/139‑1 (MQ=255)
                                                                                                     aTCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGc                             <  1:262182/139‑1 (MQ=255)
                                                                                                            ccAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCaa                      <  1:1341051/139‑1 (MQ=255)
                                                                                                                 aCCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCt                                                        >  2:533699/1‑100 (MQ=255)
                                                                                                                 aCCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCt                                                        <  1:533699/100‑1 (MQ=255)
                                                                                                                    gCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTtgt                                                                                                      >  1:932430/1‑51 (MQ=255)
                                                                                                                    gCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTtgt                                                                                                      <  2:932430/51‑1 (MQ=255)
                                                                                                                    gCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGc                             >  2:45420/1‑124 (MQ=255)
                                                                                                                    gCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGc                             <  1:45420/124‑1 (MQ=255)
                                                                                                                     ccTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCtttt                                                                                                        >  2:1679389/1‑48 (MQ=255)
                                                                                                                     ccTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCtttt                                                                                                        <  1:1679389/48‑1 (MQ=255)
                                                                                                                       tGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCtt           >  1:1245693/1‑139 (MQ=255)
                                                                                                                            aCTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATgg                  <  1:244529/127‑1 (MQ=255)
                                                                                                                            aCTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATgg                  >  2:244529/1‑127 (MQ=255)
                                                                                                                               ctcCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCa   <  2:301828/139‑1 (MQ=255)
                                                                                                                               ctcCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCa   >  1:453505/1‑139 (MQ=255)
                                                                                                                                tcCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCac  <  2:379369/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                    
TTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCAC  >  NC_000913/3176587‑3176853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑