Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,199,429 T→C A71A (GCA→GCG glnE ← fused deadenylyltransferase/adenylyltransferase for glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,199,4290TC100.0% 103.9 / NA 29A71A (GCA→GCGglnEfused deadenylyltransferase/adenylyltransferase for glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/14);  total (15/14)

CTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCATGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCG  >  NC_000913/3199301‑3199555
                                                                                                                                |                                                                                                                              
ctctctTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTa                                                                                                                        >  2:1342660/1‑137 (MQ=255)
ctctctTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTa                                                                                                                        <  1:1342660/137‑1 (MQ=255)
ctctctTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAAt                                                                                                                      >  1:440719/1‑139 (MQ=255)
        aGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGGGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCAt                                                                                                              >  1:1302513/1‑139 (MQ=255)
               aGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCcgcg                                                                                                                          >  1:1244799/1‑120 (MQ=255)
               aGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCcgcg                                                                                                                          <  2:1244799/120‑1 (MQ=255)
               aGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCgg                                                                                                       >  1:1385150/1‑139 (MQ=255)
               aGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCgg                                                                                                       >  1:1625643/1‑139 (MQ=255)
                     aGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTgcg                                                                                                 <  2:1625643/139‑1 (MQ=255)
                           tGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGtt                                                                                           >  2:125751/1‑139 (MQ=255)
                                   ggCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATtcgt                                                                                                          >  1:1734712/1‑116 (MQ=255)
                                   ggCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATtcgt                                                                                                          <  2:1734712/116‑1 (MQ=255)
                                                 tAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCa                                                                     <  1:125751/139‑1 (MQ=255)
                                                                    ccGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg                                                  <  2:1350408/139‑1 (MQ=255)
                                                                    ccGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg                                                  <  2:1385150/139‑1 (MQ=255)
                                                                        aGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGt                                              >  1:170201/1‑139 (MQ=255)
                                                                              cgcATTAACCCGGCTTCGTCACTGACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGcc                                                                                                                <  2:804247/67‑1 (MQ=255)
                                                                              cgcATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGcc                                                                                                                >  1:804247/1‑67 (MQ=255)
                                                                                  ttAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACaaa                                    <  2:440719/139‑1 (MQ=255)
                                                                                           cTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg                                                  <  2:1308002/116‑1 (MQ=255)
                                                                                           cTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg                                                  >  1:1308002/1‑116 (MQ=255)
                                                                                                   ctcacATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAagtaagta                   <  1:1232301/139‑1 (MQ=255)
                                                                                                                gcgcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg                                                  >  1:1522402/1‑95 (MQ=255)
                                                                                                                gcgcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg                                                  <  2:1522402/95‑1 (MQ=255)
                                                                                                                 cgcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg                                                  <  1:1546566/94‑1 (MQ=255)
                                                                                                                 cgcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg                                                  >  2:1546566/1‑94 (MQ=255)
                                                                                                                  gcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCtgtg    >  2:318771/1‑139 (MQ=255)
                                                                                                                    ctcctGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTgcg  >  2:1480388/1‑139 (MQ=255)
                                                                                                                    ctcctGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTgcg  <  1:1456794/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                              
CTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCATGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCG  >  NC_000913/3199301‑3199555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: