Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,240,587 A→G Y215C (TAC→TGC)  sstT → sodium:serine/threonine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,240,5870AG93.8% 207.7 / 1.7 64Y215C (TAC→TGC) sstTsodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base G (30/30);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

TGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTG  >  NC_000913/3240461‑3240718
                                                                                                                              |                                                                                                                                   
tGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAActgct                                                                                                                         >  2:321897/1‑139 (MQ=255)
 ggTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAActgctg                                                                                                                        >  1:400923/1‑139 (MQ=255)
  gTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTgg                                                                                                                       >  1:1393941/1‑139 (MQ=255)
           atGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTgg                                                                                                              <  2:400923/139‑1 (MQ=255)
            tGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGcgcg                                                                                                                                 >  1:1167807/1‑119 (MQ=255)
            tGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGcgcg                                                                                                                                 <  2:1167807/119‑1 (MQ=255)
             gTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGtt                                                                                                            <  2:1393941/139‑1 (MQ=255)
                 aaTGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTTGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCt                                                                                                        <  2:853193/139‑1 (MQ=255)
                 aaTGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCt                                                                                                        >  1:853193/1‑139 (MQ=255)
                   tGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGt                                                                                                      <  2:763817/139‑1 (MQ=255)
                   tGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGt                                                                                                      <  2:332941/139‑1 (MQ=255)
                    gCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTgg                                                                                                              <  1:134133/130‑1 (MQ=255)
                    gCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTgg                                                                                                              >  2:134133/1‑130 (MQ=255)
                        ttACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGtt                                                                                                 >  2:852318/1‑139 (MQ=255)
                               tATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACtggtg                                                                                          >  1:1593226/1‑139 (MQ=255)
                                   gTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGgcgc                                                                                      <  2:288549/139‑1 (MQ=255)
                                     gAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCtg                                                                                    <  1:852318/139‑1 (MQ=255)
                                      aaaCTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCtgg                                                                                   >  2:1018556/1‑139 (MQ=255)
                                      aaaCTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCtgg                                                                                   >  1:1150279/1‑139 (MQ=255)
                                            gTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTgg                                                                                                              <  1:1707709/106‑1 (MQ=255)
                                            gTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTgg                                                                                                              >  2:1707709/1‑106 (MQ=255)
                                            gTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTg                                                                                                                  <  1:195654/102‑1 (MQ=255)
                                            gTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTg                                                                                                                  >  2:195654/1‑102 (MQ=255)
                                               aTTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAAcc                                                                          <  2:1593226/139‑1 (MQ=255)
                                                ttcgcttcgcACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAAcc                                                                          >  1:1083519/1‑138 (MQ=255)
                                                ttcgcttcgcACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAAcc                                                                          <  2:1083519/138‑1 (MQ=255)
                                                     ttcgcACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGc                                                                    <  1:811458/139‑1 (MQ=255)
                                                      tcgcACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCTCACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTgg                                                                                                          >  2:203055/1‑100 (MQ=255)
                                                      tcgcACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCt                                                                   >  2:1350401/1‑139 (MQ=255)
                                                      tcgcACCGATTGGTATTTTTGGGCTGGTCTCTTCTACCCTGGCAACCACCGGTTTCTCCTCACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTgg                                                                                                          <  1:203055/100‑1 (MQ=255)
                                                        gcACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACT‑GTGGCGCTGGTGGTTAACCCATTGCtggt                                                                >  2:1413312/1‑139 (MQ=255)
                                                              aTTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGcgcg                                                                                                                                 <  1:1435974/69‑1 (MQ=255)
                                                              aTTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGcgcg                                                                                                                                 >  2:1435974/1‑69 (MQ=255)
                                                              aTTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTgg                                                                                                              <  1:264944/88‑1 (MQ=255)
                                                              aTTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTgg                                                                                                              >  2:264944/1‑88 (MQ=255)
                                                              aTTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTgg                                                                                                              <  2:1676250/88‑1 (MQ=255)
                                                              aTTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTgg                                                                                                              >  1:1676250/1‑88 (MQ=255)
                                                              aTTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCtggtgtggt                                                           >  1:839173/1‑139 (MQ=255)
                                                                         tGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTg                                                                                                       >  1:1104777/1‑84 (MQ=255)
                                                                         tGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTg                                                                                                       <  2:1104777/84‑1 (MQ=255)
                                                                         tGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTggtggt                                                                               >  2:737982/1‑108 (MQ=255)
                                                                         tGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTggtggt                                                                               <  1:737982/108‑1 (MQ=255)
                                                                          gggCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATtcgtc                                               <  1:321897/139‑1 (MQ=255)
                                                                            gCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTcgtcgt                                             >  2:1498905/1‑139 (MQ=255)
                                                                                gTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTg                                                                                                                  >  2:1342390/1‑66 (MQ=255)
                                                                                gTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTg                                                                                                                  <  1:1342390/66‑1 (MQ=255)
                                                                                gTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAAcc                                         >  1:616220/1‑139 (MQ=255)
                                                                                         aCCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGc                                <  1:1453452/139‑1 (MQ=255)
                                                                                           ccTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACtggtgg                                                                                         <  1:321269/80‑1 (MQ=255)
                                                                                           ccTGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACtggtgg                                                                                         >  2:321269/1‑80 (MQ=255)
                                                                                             tGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTgg                                                                                                          >  1:1165741/1‑61 (MQ=255)
                                                                                             tGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTgg                                                                                                          <  2:1165741/61‑1 (MQ=255)
                                                                                             tGGCAACCACCGGTTTCTCCACACTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGt                            <  1:1498905/139‑1 (MQ=255)
                                                                                                    caccGGTTTCTCCACACTGTGGGGCTGCGCGCAAct                                                                                                                            <  2:1413880/36‑1 (MQ=255)
                                                                                                    caccGGTTTCTCCACACTGTGGGGCTGCGCGCAAct                                                                                                                            >  1:1413880/1‑36 (MQ=255)
                                                                                                                 acacTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGaaa        <  2:1341875/139‑1 (MQ=255)
                                                                                                                   acTGTGGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACT‑GTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCg     <  1:1413312/139‑1 (MQ=255)
                                                                                                                       tgGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCGTTGCTGGTGTGGTGGAAAAtt                                                   >  1:1078107/1‑90 (MQ=255)
                                                                                                                       tgGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAAtt                                                   <  2:1078107/90‑1 (MQ=255)
                                                                                                                       tgGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGgtg  >  1:608668/1‑139 (MQ=255)
                                                                                                                       tgGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGG‑AAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCg     <  1:63765/135‑1 (MQ=255)
                                                                                                                       tgGGGCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGG‑AAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCg     >  2:63765/1‑135 (MQ=255)
                                                                                                                        ggggCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAAccc                                                                         >  2:1337171/1‑67 (MQ=255)
                                                                                                                        ggggCTGCGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAAccc                                                                         <  1:1337171/67‑1 (MQ=255)
                                                                                                                              |                                                                                                                                   
TGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTG  >  NC_000913/3240461‑3240718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑