| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,277,867 | C→T | V267I (GTC→ATC) | agaR ← | transcriptional repressor of the aga regulon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,277,867 | 0 | C | T | 97.4% | 242.4 / ‑1.2 | 76 | coding (799/810 nt) | agaR | transcriptional repressor of the aga regulon |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (36/38); minor base . (1/1); total (37/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01 | |||||||||||
TGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAA > NC_000913/3277734‑3278000 | tGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGaa > 2:310133/1‑139 (MQ=255) aaGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCGATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTc < 1:921626/139‑1 (MQ=255) agGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAAc > 2:1719980/1‑139 (MQ=255) ggACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCCCAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAAcc > 2:884927/1‑139 (MQ=255) cctcctGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGcc < 1:41396/139‑1 (MQ=255) tGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCg < 1:310133/139‑1 (MQ=255) gCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGc > 2:1474007/1‑139 (MQ=255) gACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGt < 1:252408/139‑1 (MQ=255) aCTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTc > 2:411768/1‑139 (MQ=255) ccTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCt < 1:411768/139‑1 (MQ=255) ccTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCt < 2:1331125/139‑1 (MQ=255) tCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTAt > 1:1008376/1‑139 (MQ=255) aCCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATcc < 1:884927/139‑1 (MQ=255) cccGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg > 1:115246/1‑122 (MQ=255) cccGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg < 2:115246/122‑1 (MQ=255) gaAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAAccc > 2:469773/1‑104 (MQ=255) gaAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAAccc < 1:469773/104‑1 (MQ=255) cAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg < 2:216441/113‑1 (MQ=255) cAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg > 1:216441/1‑113 (MQ=255) cAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCC‑TTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGcc < 2:304419/139‑1 (MQ=255) gaagaaACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACtt > 2:1328292/1‑92 (MQ=255) gaagaaACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACtt < 1:1328292/92‑1 (MQ=255) gaagaaACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCt > 2:846420/1‑139 (MQ=255) agaaACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtc < 2:1008376/139‑1 (MQ=255) ccATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATcaac < 1:735138/139‑1 (MQ=255) cATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGt < 2:1170018/103‑1 (MQ=255) cATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGt > 1:1170018/1‑103 (MQ=255) tgtTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACtt > 2:942183/1‑74 (MQ=255) tgtTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACtt < 1:942183/74‑1 (MQ=255) tgtTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATg > 1:82261/1‑139 (MQ=255) cacaATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCa > 1:1105328/1‑88 (MQ=255) cacaATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCa < 2:1105328/88‑1 (MQ=255) aaTGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCa > 2:461794/1‑105 (MQ=255) aaTGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCa < 1:461794/105‑1 (MQ=255) aaTGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCG‑‑‑‑‑‑ATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtc > 2:447564/1‑110 (MQ=255) aaTGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCG‑‑‑‑‑‑ATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtc < 1:447564/110‑1 (MQ=255) tGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAgg > 2:1672327/1‑96 (MQ=255) tGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAgg < 1:1672327/96‑1 (MQ=255) gCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCACAGGAATGCCTTCATCAACAATGATCATGTCGATACGtt < 1:1719980/139‑1 (MQ=255) cccGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAAt > 2:1725861/1‑58 (MQ=255) cccGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAAt < 1:1725861/58‑1 (MQ=255) ccgccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGa > 2:503277/1‑139 (MQ=255) cgccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAg < 2:1130398/139‑1 (MQ=255) gccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACg > 1:39210/1‑133 (MQ=255) gccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACg < 2:39210/133‑1 (MQ=255) gccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAACCTTTCGCAGTCCTTc > 1:1296904/1‑85 (MQ=255) gccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAACCTTTCGCAGTCCTTc < 2:1296904/85‑1 (MQ=255) ccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg > 2:212342/1‑78 (MQ=255) ccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg < 1:212342/78‑1 (MQ=255) ttCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATc < 1:1474007/139‑1 (MQ=255) cAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg > 1:1030550/1‑58 (MQ=255) cAGCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg < 2:1030550/58‑1 (MQ=255) gCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGcc < 2:1559204/83‑1 (MQ=255) gCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGcc > 1:1559204/1‑83 (MQ=255) gCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTgg < 1:846420/139‑1 (MQ=255) gCGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTgg > 2:784896/1‑139 (MQ=255) cGGGCATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGa > 1:37771/1‑139 (MQ=255) cATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTcattgttgat > 1:561950/1‑99 (MQ=255) cATCCTTTCTCA‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTcattgttgat < 2:561950/108‑10 (MQ=255) a‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGtt < 1:1056553/96‑1 (MQ=255) a‑‑CTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGtt > 2:1056553/1‑96 (MQ=255) tCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGcc < 2:239086/65‑1 (MQ=255) tCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGcc > 1:239086/1‑65 (MQ=255) tCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACg < 1:28025/92‑1 (MQ=255) tCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACg > 2:28025/1‑92 (MQ=255) tCCCCGATCAGAATCACTTC‑ACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGcc > 2:1668980/1‑64 (MQ=255) tCCCCGATCAGAATCACTTC‑ACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGcc < 1:1668980/64‑1 (MQ=255) ccccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATcaaca < 1:772086/74‑1 (MQ=255) ccccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATcaaca > 2:772086/1‑74 (MQ=255) cccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat < 1:823968/68‑1 (MQ=255) cccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat > 2:823968/1‑68 (MQ=255) cccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGa < 1:544915/86‑1 (MQ=255) cccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGa > 2:544915/1‑86 (MQ=255) cccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTAt > 2:665670/1‑98 (MQ=255) cccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTAt < 1:665670/98‑1 (MQ=255) cccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGaa > 1:1611095/1‑139 (MQ=255) | TGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCA‑‑CTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAA > NC_000913/3277734‑3278000 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |