Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,299,999 G→A A12T (GCC→ACC)  yhbQ → GIY‑YIG nuclease superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,299,9990GA100.0% 166.3 / NA 52A12T (GCC→ACC) yhbQGIY‑YIG nuclease superfamily protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/26);  total (26/26)

AACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGC  >  NC_000913/3299868‑3300122
                                                                                                                                   |                                                                                                                           
aacaTGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACaa                                                                                                                      >  1:1561191/1‑139 (MQ=255)
  caTGTTGTTTTGCCCGCCAACGGCTGATGGCGATGAGTGTTTTCATTGCTGTTCTTTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACa                                                                                                                       <  2:718746/136‑1 (MQ=255)
  caTGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACa                                                                                                                       >  1:718746/1‑136 (MQ=255)
    tgttgtTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAg                                                                                                                  >  1:221392/1‑139 (MQ=255)
             gccagccaACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATAcc                                                                                                         <  2:1561191/139‑1 (MQ=255)
             gccagccaACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATAcc                                                                                                         >  1:357573/1‑139 (MQ=255)
                       ggCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACGCCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATcacca                                                                                               <  2:221392/139‑1 (MQ=255)
                       ggCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATcacca                                                                                               <  2:283012/139‑1 (MQ=255)
                            aTGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCt                                                                                                                >  1:484296/1‑117 (MQ=255)
                            aTGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCt                                                                                                                <  2:484296/117‑1 (MQ=255)
                                              aTTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATcag                                                                        <  2:522945/139‑1 (MQ=255)
                                                              ataCTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCa                                                        <  2:357573/139‑1 (MQ=255)
                                                                    tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgggg                                                  >  1:1441190/1‑139 (MQ=255)
                                                                      ggCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAAt                                                                                                                     >  1:1706059/1‑70 (MQ=255)
                                                                      ggCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAAt                                                                                                                     <  2:1706059/70‑1 (MQ=255)
                                                                      ggCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATAcc                                                                                                         <  2:701806/82‑1 (MQ=255)
                                                                      ggCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATAcc                                                                                                         >  1:701806/1‑82 (MQ=255)
                                                                      ggCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCCAAGGGGCg                                                >  2:1057680/1‑139 (MQ=255)
                                                                      ggCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCg                                                >  1:1693908/1‑139 (MQ=255)
                                                                           gACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGc                                           >  1:587514/1‑139 (MQ=255)
                                                                            aCCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAAt                                                                                                                     <  1:55918/64‑1 (MQ=255)
                                                                            aCCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAAt                                                                                                                     >  2:55918/1‑64 (MQ=255)
                                                                                   cATGCGACGCAGACGATGACGCCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATcagca                                                                      <  2:1298827/104‑1 (MQ=255)
                                                                                   cATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATcagca                                                                      >  1:1298827/1‑104 (MQ=255)
                                                                                       cGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCt                                                                                                                >  2:336140/1‑58 (MQ=255)
                                                                                       cGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCt                                                                                                                <  1:336140/58‑1 (MQ=255)
                                                                                       cGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATc                                                                                                   >  2:32815/1‑71 (MQ=255)
                                                                                       cGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATc                                                                                                   <  1:32815/71‑1 (MQ=255)
                                                                                       cGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAAcgccg                                                                               <  2:1429362/91‑1 (MQ=255)
                                                                                       cGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAAcgccg                                                                               >  1:1429362/1‑91 (MQ=255)
                                                                                       cGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATcag                                                                        <  2:911964/98‑1 (MQ=255)
                                                                                       cGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATcag                                                                        >  1:911964/1‑98 (MQ=255)
                                                                                        gACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCa                                          <  2:881730/127‑1 (MQ=255)
                                                                                        gACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCa                                          >  1:881730/1‑127 (MQ=255)
                                                                                          cGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgg                                                    <  2:897451/115‑1 (MQ=255)
                                                                                          cGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAgg                            <  2:1657360/139‑1 (MQ=255)
                                                                                          cGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTGATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgg                                                    >  1:897451/1‑115 (MQ=255)
                                                                                              gACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGaa                        >  2:1585113/1‑139 (MQ=255)
                                                                                               aCGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATcacca                                                                                               >  2:727282/1‑67 (MQ=255)
                                                                                               aCGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATcacca                                                                                               <  1:727282/67‑1 (MQ=255)
                                                                                                              tttCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAAcg                                                                                  <  2:162548/65‑1 (MQ=255)
                                                                                                              tttCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAAcg                                                                                  >  1:162548/1‑65 (MQ=255)
                                                                                                                    tACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCATTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCgcgc  <  2:476489/139‑1 (MQ=255)
                                                                                                                    tACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCgcgc  <  1:1585113/139‑1 (MQ=255)
                                                                                                                          aTCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAAcg                                                                                  >  1:882697/1‑53 (MQ=255)
                                                                                                                          aTCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAAcg                                                                                  <  2:882697/53‑1 (MQ=255)
                                                                                                                          aTCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATcag                                                                        <  1:14376/63‑1 (MQ=255)
                                                                                                                          aTCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATcag                                                                        >  2:14376/1‑63 (MQ=255)
                                                                                                                           tCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAGGGGCGAAAGCa                                          >  1:135014/1‑91 (MQ=255)
                                                                                                                           tCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAGGGGCGAAAGCa                                          <  2:135014/91‑1 (MQ=255)
                                                                                                                               tACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGaa                                                                                    <  2:87397/46‑1 (MQ=255)
                                                                                                                               tACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGaa                                                                                    >  1:87397/1‑46 (MQ=255)
                                                                                                                                   |                                                                                                                           
AACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGC  >  NC_000913/3299868‑3300122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: