Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,301,599 G→A G39S (GGC→AGC)  yhbU → U32 peptidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,301,5990GA100.0% 179.0 / NA 55G39S (GGC→AGC) yhbUU32 peptidase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/25);  total (30/25)

ACCTTCAGGAAAAATTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCGGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGTGGATA  >  NC_000913/3301469‑3301731
                                                                                                                                  |                                                                                                                                    
aCCTTCAGGAAAAATTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAAc                                                                                                                              >  1:978034/1‑139 (MQ=255)
 ccTTCAGGAAAAATTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGc                                                                                                                                    >  1:1551046/1‑132 (MQ=255)
 ccTTCAGGAAAAATTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGc                                                                                                                                    <  2:1551046/132‑1 (MQ=255)
     cAGGAAAAATTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTAc                                                                                                                         <  2:1626662/139‑1 (MQ=255)
           aaaTTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGaa                                                                                                                   >  2:252864/1‑139 (MQ=255)
                        gctCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGaa                                                                                                      <  1:252864/139‑1 (MQ=255)
                                ctgccGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCgaga                                                                                                                    <  1:716054/117‑1 (MQ=255)
                                ctgccGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCgaga                                                                                                                    >  2:716054/1‑117 (MQ=255)
                                   ccGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGttt                                                                                           >  2:710917/1‑139 (MQ=255)
                                             cccGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCgaga                                                                                                                    <  2:764530/104‑1 (MQ=255)
                                             cccGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCgaga                                                                                                                    >  1:764530/1‑104 (MQ=255)
                                                      tAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATcgccgc                                                                        <  1:710917/139‑1 (MQ=255)
                                                              ccATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATcgccgc                                                                        <  2:1399073/131‑1 (MQ=255)
                                                              ccATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATcgccgc                                                                        >  1:1399073/1‑131 (MQ=255)
                                                              ccATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACt                                                                   <  2:443579/136‑1 (MQ=255)
                                                              ccATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACt                                                                   >  1:443579/1‑136 (MQ=255)
                                                                           cgcAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCgg                                                                                                  <  2:1111890/92‑1 (MQ=255)
                                                                           cgcAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCgg                                                                                                  >  1:1111890/1‑92 (MQ=255)
                                                                           cgcAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACa                                                                               >  1:1646918/1‑111 (MQ=255)
                                                                           cgcAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACa                                                                               <  2:1646918/111‑1 (MQ=255)
                                                                                     gTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGc                                                                                                    <  1:814671/80‑1 (MQ=255)
                                                                                     gTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGc                                                                                                    >  2:814671/1‑80 (MQ=255)
                                                                                           atCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCgg                                   <  2:978034/139‑1 (MQ=255)
                                                                                             cGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATcgcc                                                                          >  2:670247/1‑98 (MQ=255)
                                                                                             cGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATcgcc                                                                          <  1:670247/98‑1 (MQ=255)
                                                                                              gggCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTg                                                                                                            <  2:984355/63‑1 (MQ=255)
                                                                                              gggCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTg                                                                                                            >  1:984355/1‑63 (MQ=255)
                                                                                               ggCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGtt                                                                                            >  1:979960/1‑78 (MQ=255)
                                                                                               ggCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGtt                                                                                            <  2:979960/78‑1 (MQ=255)
                                                                                                gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCt                                                                                                                                  >  1:26603/1‑39 (MQ=255)
                                                                                                gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCt                                                                                                                                  <  2:26603/39‑1 (MQ=255)
                                                                                                gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACAt                                                             <  2:831077/108‑1 (MQ=255)
                                                                                                gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACAt                                                             >  1:831077/1‑108 (MQ=255)
                                                                                                gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGAtt                                                      <  1:1679211/115‑1 (MQ=255)
                                                                                                gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGAtt                                                      >  2:1679211/1‑115 (MQ=255)
                                                                                                          gatACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGtt                    >  1:1062550/1‑139 (MQ=255)
                                                                                                             aCCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGc                 >  2:1537524/1‑139 (MQ=255)
                                                                                                               cAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCaa                                                                      >  2:82594/1‑84 (MQ=255)
                                                                                                               cAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCaa                                                                      <  1:82594/84‑1 (MQ=255)
                                                                                                                 aTGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGT‑CATCAACATCGCCGCAAACTTCACATCGCGATTaa                                                    <  2:192809/99‑1 (MQ=255)
                                                                                                                 aTGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGT‑CATCAACATCGCCGCAAACTTCACATCGCGATTaa                                                    >  1:192809/1‑99 (MQ=255)
                                                                                                                  tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTg                                                                                         <  2:24514/62‑1 (MQ=255)
                                                                                                                  tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTg                                                                                         <  1:1189327/62‑1 (MQ=255)
                                                                                                                  tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTg                                                                                         >  2:1189327/1‑62 (MQ=255)
                                                                                                                  tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTg                                                                                         >  1:24514/1‑62 (MQ=255)
                                                                                                                  tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTg                                            <  2:955778/107‑1 (MQ=255)
                                                                                                                  tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTg                                            >  1:955778/1‑107 (MQ=255)
                                                                                                                      cGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTgcgc                                         <  2:314616/106‑1 (MQ=255)
                                                                                                                      cGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTgcgc                                         >  1:314616/1‑106 (MQ=255)
                                                                                                                       gTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGt       >  2:633640/1‑139 (MQ=255)
                                                                                                                       gTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGt       >  2:231023/1‑139 (MQ=255)
                                                                                                                            ttCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGtt                                                                                            <  1:1174345/49‑1 (MQ=255)
                                                                                                                            ttCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGtt                                                                                            >  2:1174345/1‑49 (MQ=255)
                                                                                                                            ttCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGTGGata  >  1:1568183/1‑139 (MQ=255)
                                                                                                                            ttCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGTGGata  >  1:1684374/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                                    
ACCTTCAGGAAAAATTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCGGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGTGGATA  >  NC_000913/3301469‑3301731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑