Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,330,396 G→A *478* (TAG→TAA)  dacB → D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,330,3960GA100.0% 148.0 / NA 46*478* (TAG→TAA) dacBD‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/23);  total (23/23)

AGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTA  >  NC_000913/3330261‑3330531
                                                                                                                                        |                                                                                                                                       
agcgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATc                                                                                                                                       >  1:923545/1‑139 (MQ=255)
       gggCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGa                                                                                                                                >  2:782483/1‑139 (MQ=255)
       gggCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGa                                                                                                                                <  1:782483/139‑1 (MQ=255)
            aCGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAAccc                                                                                                                           <  1:1273305/139‑1 (MQ=255)
            aCGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAAccc                                                                                                                           <  2:1125544/139‑1 (MQ=255)
                      tttGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAg                                                                                                                                 >  1:1040746/1‑123 (MQ=255)
                      tttGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAg                                                                                                                                 <  2:1040746/123‑1 (MQ=255)
                          tgCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCgg                                                                                                             <  2:706414/139‑1 (MQ=255)
                                   tttCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGa                                                                                                    <  2:923545/139‑1 (MQ=255)
                                           tATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGatta                                                                                                 <  2:596920/134‑1 (MQ=255)
                                           tATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGatta                                                                                                 >  1:596920/1‑134 (MQ=255)
                                             tGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACaa                                                                                          >  2:253725/1‑139 (MQ=255)
                                              gCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACaaa                                                                                         <  1:1020953/139‑1 (MQ=255)
                                                          gCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTg                                                                                      <  1:1600458/130‑1 (MQ=255)
                                                          gCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTg                                                                                      >  2:1600458/1‑130 (MQ=255)
                                                           cGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGttt                                                                            >  1:668389/1‑139 (MQ=255)
                                                                  gCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAg                                                                                                     <  2:128516/107‑1 (MQ=255)
                                                                  gCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAg                                                                                                     >  1:128516/1‑107 (MQ=255)
                                                                     tAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATg                                                                  >  2:1598053/1‑139 (MQ=255)
                                                                        tCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAAccc                                                                                                                           >  2:1583806/1‑79 (MQ=255)
                                                                        tCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAAccc                                                                                                                           <  1:1583806/79‑1 (MQ=255)
                                                                             gTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGatta                                                                                                 >  1:269131/1‑100 (MQ=255)
                                                                             gTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGatta                                                                                                 <  2:269131/100‑1 (MQ=255)
                                                                                      tAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTc                                                                                                       >  1:858098/1‑85 (MQ=255)
                                                                                      tAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTc                                                                                                       <  2:858098/85‑1 (MQ=255)
                                                                                             tttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                          >  1:152294/2‑139 (MQ=255)
                                                                                                     aGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCg                                              <  1:1640092/127‑1 (MQ=255)
                                                                                                     aGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCg                                              >  2:1640092/1‑127 (MQ=255)
                                                                                                         gTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc                              <  2:668389/139‑1 (MQ=255)
                                                                                                          tttGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGcc                                                                                                               >  2:281189/1‑57 (MQ=255)
                                                                                                          tttGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGcc                                                                                                               <  1:281189/57‑1 (MQ=255)
                                                                                                                        tttATCAGAACAATTAATCAAAAAGAAACCCCGGca                                                                                                                      <  1:1367083/36‑1 (MQ=255)
                                                                                                                        tttATCAGAACAATTAATCAAAAAGAAACCCCGGca                                                                                                                      >  2:1367083/1‑36 (MQ=255)
                                                                                                                        tttATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCg                                                                                                              <  1:551658/44‑1 (MQ=255)
                                                                                                                        tttATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCg                                                                                                              >  2:551658/1‑44 (MQ=255)
                                                                                                                        tttATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGat                                                                                                   >  2:759437/1‑55 (MQ=255)
                                                                                                                        tttATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGat                                                                                                   <  1:759437/55‑1 (MQ=255)
                                                                                                                        tttATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                                           <  1:685523/95‑1 (MQ=255)
                                                                                                                        tttATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                                           >  2:685523/1‑95 (MQ=255)
                                                                                                                        tttATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTg               <  2:152294/139‑1 (MQ=255)
                                                                                                                           aTCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCaa            >  1:767662/1‑139 (MQ=255)
                                                                                                                              aGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtc         >  1:539874/1‑139 (MQ=255)
                                                                                                                                aaCAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaa                                     >  2:1527041/1‑109 (MQ=255)
                                                                                                                                aaCAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaa                                     <  1:1527041/109‑1 (MQ=255)
                                                                                                                                   aaTTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTg    <  1:1598053/139‑1 (MQ=255)
                                                                                                                                     ttAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTa  >  1:32028/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                       
AGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTA  >  NC_000913/3330261‑3330531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑