Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,418,548 T→C A53A (GCT→GCC yhdV → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,418,5480TC94.2% 285.2 / 3.3 87A53A (GCT→GCCyhdVputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base C (40/42);  total (42/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.71e-01

CCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATA  >  NC_000913/3418421‑3418683
                                                                                                                               |                                                                                                                                       
ccGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCcggcgg                                                                                                                              <  2:1608918/139‑1 (MQ=255)
 cGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCggg                                                                                                                             >  1:851020/1‑139 (MQ=255)
      tGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAgc                                                                                                                        >  1:641787/1‑139 (MQ=255)
      tGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAgc                                                                                                                        >  2:763071/1‑139 (MQ=255)
       gCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAgct                                                                                                                       >  1:1676725/1‑139 (MQ=255)
         tGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAgctgc                                                                                                                     <  1:675382/139‑1 (MQ=255)
           gCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGctgctg                                                                                                                   >  1:797442/1‑139 (MQ=255)
                 tgtgCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTg                                                                                                             <  1:763071/139‑1 (MQ=255)
                         cGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACg                                                                                                     <  1:372995/139‑1 (MQ=255)
                              cGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATggg                                                                                                <  2:1676725/139‑1 (MQ=255)
                                   tGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATGACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGatca                                                                                           <  2:504292/139‑1 (MQ=255)
                                    gCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCt                                                                                                              <  2:417702/119‑1 (MQ=255)
                                    gCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCt                                                                                                              >  1:417702/1‑119 (MQ=255)
                                    gCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGatcat                                                                                          >  2:5678/1‑139 (MQ=255)
                                     cGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGatcatc                                                                                         >  1:1235394/1‑139 (MQ=255)
                                                 tGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGaaa                                                                             <  1:5678/139‑1 (MQ=255)
                                                    cGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATaa                                                                          <  2:1439278/139‑1 (MQ=255)
                                                       tCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGct                                                                                      <  1:1345726/124‑1 (MQ=255)
                                                       tCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGct                                                                                      >  2:1345726/1‑124 (MQ=255)
                                                         aGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGaa                                                                     <  2:797442/139‑1 (MQ=255)
                                                               gtcTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTgg                                                                                                            <  2:1545176/94‑1 (MQ=255)
                                                               gtcTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTgg                                                                                                            >  1:1545176/1‑94 (MQ=255)
                                                                tcTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGgcg                                                              >  2:1589297/1‑139 (MQ=255)
                                                                          acCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCcg                                                    >  2:860669/1‑139 (MQ=255)
                                                                          acCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCAAAATAACTGAACAGGGCGCGGAGGTCcg                                                    >  1:1037999/1‑139 (MQ=255)
                                                                              aTACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGct                                                >  1:503616/1‑139 (MQ=255)
                                                                              aTACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGct                                                >  1:1417724/1‑139 (MQ=255)
                                                                               tACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGct                                                                                      <  1:600539/100‑1 (MQ=255)
                                                                               tACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGct                                                                                      >  2:600539/1‑100 (MQ=255)
                                                                                 ccTGTATGAAAGGCTCGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCATCTGCTGTTGCTGGCCTGACGATGGTGATCATCGCTCTATCGAAATAACTGATCAGGGCGCGGAGTTCCACGCTCtt                                             <  1:1059710/139‑1 (MQ=255)
                                                                                    gTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGaa                                                                     >  1:977046/1‑112 (MQ=255)
                                                                                    gTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGaa                                                                     <  2:977046/112‑1 (MQ=255)
                                                                                    gTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGcgcg                                                            <  2:81695/121‑1 (MQ=255)
                                                                                    gTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGcgcg                                                            >  1:81695/1‑121 (MQ=255)
                                                                                    gTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCttttt                                          >  2:671/1‑139 (MQ=255)
                                                                                     tATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGct                                                                                      <  1:265896/94‑1 (MQ=255)
                                                                                     tATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGct                                                                                      >  2:265896/1‑94 (MQ=255)
                                                                                      tataagagacagACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTaa                                        <  1:480195/128‑1 (MQ=255)
                                                                                      aTGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAgctg                                                                                                                      <  1:905219/61‑1 (MQ=255)
                                                                                      aTGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAgctg                                                                                                                      >  2:905219/1‑61 (MQ=255)
                                                                                      aTGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTaa                                        >  1:206651/1‑139 (MQ=255)
                                                                                      aTGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTaa                                        <  1:860669/139‑1 (MQ=255)
                                                                                      aTGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTaa                                        <  2:206651/139‑1 (MQ=255)
                                                                                       tGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCcggcgg                                                                                                                              <  1:1205368/52‑1 (MQ=255)
                                                                                       tGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCcggcgg                                                                                                                              >  2:1205368/1‑52 (MQ=255)
                                                                                       tGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAAc                                       <  2:1235394/139‑1 (MQ=255)
                                                                                         aaaGGCACGACTCAGGATGACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGa                                     >  1:931062/1‑139 (MQ=255)
                                                                                          aaGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAgg                                                                 >  1:1434537/1‑110 (MQ=255)
                                                                                          aaGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAgg                                                                 <  2:1434537/110‑1 (MQ=255)
                                                                                           aGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGa                                                                               >  1:85599/1‑95 (MQ=255)
                                                                                           aGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGa                                                                               <  2:85599/95‑1 (MQ=255)
                                                                                           aGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATa                                   <  2:1700220/139‑1 (MQ=255)
                                                                                           aGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATa                                   <  2:1417724/139‑1 (MQ=255)
                                                                                           aGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATa                                   <  2:503616/139‑1 (MQ=255)
                                                                                             gCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCcggc                                                                                                                                >  1:1079564/1‑44 (MQ=255)
                                                                                             gCACGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCcggc                                                                                                                                <  2:1079564/44‑1 (MQ=255)
                                                                                               aCGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTg                                                                                                        >  2:523524/1‑66 (MQ=255)
                                                                                               aCGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTg                                                                                                        <  1:523524/66‑1 (MQ=255)
                                                                                               aCGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGaaa                                                                             >  2:183871/1‑93 (MQ=255)
                                                                                               aCGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGaaa                                                                             <  1:183871/93‑1 (MQ=255)
                                                                                                cGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCa                                                                                                                          <  1:500134/47‑1 (MQ=255)
                                                                                                cGACTCAGGATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCa                                                                                                                          >  2:500134/1‑47 (MQ=255)
                                                                                                       ggATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTAt                             <  2:1219450/133‑1 (MQ=255)
                                                                                                       ggATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTAt                             >  1:1219450/1‑133 (MQ=255)
                                                                                                        gATAACTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCAGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTAtctctcta                      <  2:1037999/139‑3 (MQ=255)
                                                                                                         atgaCTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCAt                     <  2:931062/136‑1 (MQ=255)
                                                                                                             cTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAgctg                                                                                                                      <  2:1671287/38‑1 (MQ=255)
                                                                                                             cTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAgctg                                                                                                                      >  1:1671287/1‑38 (MQ=255)
                                                                                                             cTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATATCTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTaa                                        >  1:408243/1‑116 (MQ=255)
                                                                                                             cTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATATCTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTaa                                        <  2:408243/116‑1 (MQ=255)
                                                                                                             cTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGaa                                                                     <  1:689409/87‑1 (MQ=255)
                                                                                                             cTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGaa                                                                     >  2:689409/1‑87 (MQ=255)
                                                                                                             cTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCcgcg                                                  >  2:467758/1‑106 (MQ=255)
                                                                                                             cTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCcgcg                                                  <  1:467758/106‑1 (MQ=255)
                                                                                                             cTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAAc                                       >  1:685165/1‑117 (MQ=255)
                                                                                                             cTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAAc                                       <  2:685165/117‑1 (MQ=255)
                                                                                                             cTGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGATGATGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAAAAGGGCCCGGGGTTCCGCGCTCTTTTAAACGAAACAGTAactgactcttata                 >  2:480195/1‑129 (MQ=255)
                                                                                                              tGGGAAACGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGAt                >  1:1310329/1‑139 (MQ=255)
                                                                                                                  aaaCGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATaaaa            >  1:9911/1‑139 (MQ=255)
                                                                                                                  aaaCGGCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATaaaa            >  1:236532/1‑139 (MQ=255)
                                                                                                                    aCGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAata          <  2:641787/139‑1 (MQ=255)
                                                                                                                       gCTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAGCAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTg       <  1:671/139‑1 (MQ=255)
                                                                                                                        cTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGa                                                                               <  2:420951/66‑1 (MQ=255)
                                                                                                                        cTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGa                                                                               >  1:420951/1‑66 (MQ=255)
                                                                                                                        cTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTg       >  1:1347158/1‑138 (MQ=255)
                                                                                                                        cTGGGGCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTg       <  2:1347158/138‑1 (MQ=255)
                                                                                                                            ggCCATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCtata  <  2:236532/139‑1 (MQ=255)
                                                                                                                               |                                                                                                                                       
CCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATA  >  NC_000913/3418421‑3418683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: