| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,478,592 | C→T | *67* (TAG→TAA) | yheV ← | DUF2387 family putative metal‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,478,592 | 0 | C | T | 98.6% | 227.7 / NA | 70 | *67* (TAG→TAA) | yheV | DUF2387 family putative metal‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (34/35); minor base A (1/0); total (35/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGCTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATC > NC_000913/3478461‑3478702 | aGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCg > 2:1403492/1‑139 (MQ=255) ggCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCgg < 2:755846/139‑1 (MQ=255) accaccAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATc > 1:1725081/1‑139 (MQ=255) aGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATc < 2:1725081/139‑1 (MQ=255) cTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACtt > 1:1101686/1‑139 (MQ=255) cTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACtt > 1:645480/1‑139 (MQ=255) ttGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCTCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGa < 2:1112808/120‑1 (MQ=255) ttGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATAGCGTTAGTCCGGATGa > 1:1112808/1‑120 (MQ=255) tatCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGt < 1:134226/139‑1 (MQ=255) aGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTc > 2:468666/1‑89 (MQ=255) aGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTc < 1:468666/89‑1 (MQ=255) gCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGc < 2:645480/139‑1 (MQ=255) cATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGct < 1:1403492/139‑1 (MQ=255) aTGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGctt > 1:1359330/1‑139 (MQ=255) gggAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCAt > 2:262195/1‑113 (MQ=255) gggAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCAt < 1:262195/113‑1 (MQ=255) gggAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCgg < 2:520518/133‑1 (MQ=255) gggAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCgg > 1:520518/1‑133 (MQ=255) gggAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGcttct > 2:815145/1‑139 (MQ=255) gggAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGcttct > 2:798767/1‑139 (MQ=255) ggAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTc < 2:667936/83‑1 (MQ=255) ggAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTc > 1:667936/1‑83 (MQ=255) aTAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGc < 2:1166180/112‑1 (MQ=255) aTAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGc > 1:1166180/1‑112 (MQ=255) aTAGTGGCGCAGATTGTAGCGGAAATATGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGATAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTg > 2:826500/1‑139 (MQ=255) tAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGc > 2:129733/1‑139 (MQ=255) tGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCgg > 2:1221404/1‑122 (MQ=255) tGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCgg < 1:1221404/122‑1 (MQ=255) ggCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGaa < 2:1228090/80‑1 (MQ=255) ggCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGaa > 1:1228090/1‑80 (MQ=255) gcgcAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTtctc < 1:826500/139‑1 (MQ=255) cAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGaa < 1:1419643/106‑1 (MQ=255) cAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGaa > 2:1419643/1‑106 (MQ=255) gCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTc < 2:1359330/139‑1 (MQ=255) cGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTcc < 1:815145/139‑1 (MQ=255) ggAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCg < 1:1590583/139‑1 (MQ=255) aaTTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCa > 1:82428/1‑139 (MQ=255) cTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGc < 1:1517223/134‑1 (MQ=255) cTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGc > 2:1517223/1‑134 (MQ=255) tGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCtt > 1:647006/1‑115 (MQ=255) tGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCtt < 2:647006/115‑1 (MQ=255) gCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAAcaca < 2:82428/139‑1 (MQ=255) gtgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGc < 2:965589/68‑1 (MQ=255) gtgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGc > 1:965589/1‑68 (MQ=255) tgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCTCTTGCTCATCTTTGCGAACGTGATCGCgg > 2:446999/1‑91 (MQ=255) tgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCAc < 1:1617263/63‑1 (MQ=255) tgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCAc > 2:1617263/1‑63 (MQ=255) tgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCgg < 1:446999/91‑1 (MQ=255) tgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAAcaca < 1:81561/135‑1 (MQ=255) tgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAAcaca > 2:81561/1‑135 (MQ=255) tACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCgg > 2:1091820/1‑43 (MQ=255) tACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCgg < 1:1091820/43‑1 (MQ=255) aCTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCg < 2:1293857/104‑1 (MQ=255) aCTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCg > 1:1293857/1‑104 (MQ=255) tAGCTTAAAAAAACTCGGCGGATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAAcaatatc < 2:1014061/138‑1 (MQ=255) tAGCTTAAAAAAACTCGGCGGATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAAcaatatc > 1:1014061/1‑138 (MQ=255) tAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTcc < 1:781126/114‑1 (MQ=255) tAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTcc > 2:781126/1‑114 (MQ=255) tAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTcaaca < 2:551393/133‑1 (MQ=255) tAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTcaaca > 1:551393/1‑133 (MQ=255) tAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAAcaata < 2:1399032/136‑1 (MQ=255) tAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAAcaata > 1:1399032/1‑136 (MQ=255) aGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTcc < 2:203941/113‑1 (MQ=255) aGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTcc > 1:203941/1‑113 (MQ=255) cTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATc > 2:671864/1‑75 (MQ=255) cTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATc < 1:671864/75‑1 (MQ=255) aaaaaaaCTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCgg > 2:1073445/1‑76 (MQ=255) aaaaaaaCTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCgg < 1:1073445/76‑1 (MQ=255) aaaaaCTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATc > 2:1105622/1‑44 (MQ=255) aaaaaCTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATc < 1:1105622/44‑1 (MQ=255) | AGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGCTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATC > NC_000913/3478461‑3478702 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |