Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,482,967	T→C	L560P (CTG→CCG)	yheS →	ABC‑F family protein predicted regulatory ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,482,967	0	T	C	100.0%	213.1 / NA	58	L560P (CTG→CCG)	yheS	ABC‑F family protein predicted regulatory ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/30);  total (28/30)

ATCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCCTGCCTGCAAC  >  NC_000913/3482832‑3483101                                                                                                                                        |                                                                                                                                       aTCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACcgcg                                                                                                                                     >  2:23144/1‑139 (MQ=255)   cAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTa                                                                                                                                   <  2:1483009/139‑1 (MQ=255)   cAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTa                                                                                                                                   <  1:1649658/139‑1 (MQ=255)        gTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAagag                                                                                                                              >  1:1620123/1‑139 (MQ=255)         tGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAgaga                                                                                                                             >  2:860702/1‑139 (MQ=255)                              cAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAgaga                                                                                                        <  2:221183/139‑1 (MQ=255)                                ggAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGaaaaa                                                                                                            >  2:1320731/1‑133 (MQ=255)                                ggAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGaaaaa                                                                                                            <  1:1320731/133‑1 (MQ=255)                                ggAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGATGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATg                                                                                                      >  2:1097675/1‑139 (MQ=255)                                ggAAAACCAGACCGACGAAGCACCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATg                                                                                                      >  1:1636588/1‑139 (MQ=255)                                   aaaCCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGgag                                                                                                   <  1:860702/139‑1 (MQ=255)                                        aGACCGACGAAGCGCCAAAAGAGAATGCGAATAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCt                                                                                              <  1:1145971/139‑1 (MQ=255)                                           ccGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGaa                                                                                           <  2:1620123/139‑1 (MQ=255)                                               cgaAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTg                                                                                                                          >  2:114087/1‑104 (MQ=255)                                               cgaAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTg                                                                                                                          <  1:114087/104‑1 (MQ=255)                                                            gagaATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAgg                                                                          <  2:1189753/139‑1 (MQ=255)                                                              gaATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCg                                                                        >  1:118626/1‑139 (MQ=255)                                                                   cGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAaga                                                                   >  2:740313/1‑139 (MQ=255)                                                                         gcgcCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACt                                                             >  1:4311/1‑139 (MQ=255)                                                                                    cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCg                                                  >  1:1510383/1‑139 (MQ=255)                                                                                    cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCg                                                  <  2:1636588/139‑1 (MQ=255)                                                                                    cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCg                                                  <  1:836352/139‑1 (MQ=255)                                                                                     gTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                 <  2:267425/139‑1 (MQ=255)                                                                                     gTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                 <  2:118626/139‑1 (MQ=255)                                                                                     gTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                 <  1:740313/139‑1 (MQ=255)                                                                                     gTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                 <  2:2299/139‑1 (MQ=255)                                                                                      tAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCgg                                                          >  2:900328/1‑129 (MQ=255)                                                                                      tAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCgg                                                          <  1:900328/129‑1 (MQ=255)                                                                                          gATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGt                                            <  1:1097675/139‑1 (MQ=255)                                                                                           aTCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCg                                                  >  1:1312411/1‑132 (MQ=255)                                                                                           aTCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCg                                                  <  2:1312411/132‑1 (MQ=255)                                                                                             cAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGgcg                                                                              >  1:563782/1‑102 (MQ=255)                                                                                             cAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGgcg                                                                              <  2:563782/102‑1 (MQ=255)                                                                                                aaGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAACGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                 >  1:1076731/1‑128 (MQ=255)                                                                                                aaGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATg                                                                                                      <  2:1453028/75‑1 (MQ=255)                                                                                                aaGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATg                                                                                                      >  1:1453028/1‑75 (MQ=255)                                                                                                aaGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                 <  2:1076731/128‑1 (MQ=255)                                                                                                     tCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAgaga                                                                                                        <  2:292550/68‑1 (MQ=255)                                                                                                     tCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAgaga                                                                                                        >  1:292550/1‑68 (MQ=255)                                                                                                     tCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAAcg                                                                                         >  1:761825/1‑83 (MQ=255)                                                                                                     tCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAAcg                                                                                         <  2:761825/83‑1 (MQ=255)                                                                                                     tCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGc                                 >  2:511268/1‑139 (MQ=255)                                                                                                     tCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGc                                 >  2:481965/1‑139 (MQ=255)                                                                                                        ggAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCt                                                                                                                   <  1:1378522/54‑1 (MQ=255)                                                                                                        ggAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCt                                                                                                                   >  2:1378522/1‑54 (MQ=255)                                                                                                            gctgagctgCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAg                          >  2:436750/1‑139 (MQ=255)                                                                                                               gagctgCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAAcg                                                                                         <  1:1643731/73‑1 (MQ=255)                                                                                                               gagctgCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAAcg                                                                                         >  2:1643731/1‑73 (MQ=255)                                                                                                               gagctgCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCagag                                  >  1:1371994/1‑128 (MQ=255)                                                                                                               gagctgCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCagag                                  <  2:1371994/128‑1 (MQ=255)                                                                                                                agctgCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                 >  2:364233/1‑112 (MQ=255)                                                                                                                agctgCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                 <  1:364233/112‑1 (MQ=255)                                                                                                                 gctgCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAg                     >  2:144952/1‑139 (MQ=255)                                                                                                                  ctgCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                 <  2:726753/110‑1 (MQ=255)                                                                                                                  ctgCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                 >  1:726753/1‑110 (MQ=255)                                                                                                                         cgcAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCgc             <  2:1510383/139‑1 (MQ=255)                                                                                                                          gcAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCgcc            >  2:525505/1‑139 (MQ=255)                                                                                                                                 cAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCctgcctgc     <  1:144952/139‑1 (MQ=255)                                                                                                                                    ccACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCCTGCCTGCAAc  >  2:532332/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                       ATCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCCTGCCTGCAAC  >  NC_000913/3482832‑3483101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑