Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,545,565	G→A	*228* (TAG→TAA)	gntX →	DNA catabolic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,545,565	0	G	A	100.0%	253.3 / NA	77	*228* (TAG→TAA)	gntX	DNA catabolic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/41);  total (36/41)

CCGTGCAAGGTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAGAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCAAACTGCTGGCAAATCAGGAAGAAGGGACACAAATCC  >  NC_000913/3545432‑3545699                                                                                                                                      |                                                                                                                                       ccGTGCAAGGTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCt                                                                                                                                   <  1:520052/139‑1 (MQ=255)     gCAAGGTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgat                                                                                                                               >  2:501751/1‑139 (MQ=255)       aaGGTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                                             <  1:1124199/139‑1 (MQ=255)       aaGGTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                                             <  1:99995/139‑1 (MQ=255)       aaGGTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                                             <  1:1273463/139‑1 (MQ=255)          gTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatgat                                                                                                                            >  1:866796/1‑137 (MQ=255)          gTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatgat                                                                                                                            <  2:866796/137‑1 (MQ=255)           tCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATggg                                                                                                                         <  1:326694/139‑1 (MQ=255)            cGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                                        <  1:61866/139‑1 (MQ=255)            cGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                                        <  1:398376/139‑1 (MQ=255)                                   tgTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGt                                                                                                 >  2:279578/1‑139 (MQ=255)                                    gTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTTGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTc                                                                                                <  1:279578/139‑1 (MQ=255)                                    gTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTc                                                                                                >  2:153315/1‑139 (MQ=255)                                               cGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                                     >  2:1456321/1‑139 (MQ=255)                                                      aCCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACt                                                                              <  1:1456321/139‑1 (MQ=255)                                                        cGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAAcc                                                                                                             >  2:132680/1‑106 (MQ=255)                                                        cGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAAcc                                                                                                             <  1:132680/106‑1 (MQ=255)                                                           cGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCt                                                                                                                                   <  2:1616586/81‑1 (MQ=255)                                                           cGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCt                                                                                                                                   >  1:1616586/1‑81 (MQ=255)                                                           cGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGAt                                                                         <  1:187471/139‑1 (MQ=255)                                                           cGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGAt                                                                         >  2:187471/1‑139 (MQ=255)                                                           cGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGAt                                                                         >  1:765247/1‑139 (MQ=255)                                                             cAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATcc                                                                       >  2:576499/1‑139 (MQ=255)                                                                  aTTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatgat                                                                                                                            >  1:213050/1‑81 (MQ=255)                                                                  aTTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatgat                                                                                                                            <  2:213050/81‑1 (MQ=255)                                                                    tGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatgat                                                                                                                            >  1:321904/1‑79 (MQ=255)                                                                    tGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatgat                                                                                                                            <  2:321904/79‑1 (MQ=255)                                                                    tGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatg                                                                                                                              <  1:1273876/77‑1 (MQ=255)                                                                    tGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatg                                                                                                                              >  2:1273876/1‑77 (MQ=255)                                                                    tGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATg                                                                           >  1:554460/1‑128 (MQ=255)                                                                    tGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATg                                                                           <  2:554460/128‑1 (MQ=255)                                                                      cgcAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATggg                                                                                                                         >  1:679401/1‑80 (MQ=255)                                                                      cgcAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATggg                                                                                                                         <  2:679401/80‑1 (MQ=255)                                                                      cgcAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCg                                                              >  2:55395/1‑139 (MQ=255)                                                                            tGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                                        <  2:26282/75‑1 (MQ=255)                                                                            tGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                                        >  1:26282/1‑75 (MQ=255)                                                                            tGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                                     >  2:1555066/1‑110 (MQ=255)                                                                            tGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                                     <  1:1555066/110‑1 (MQ=255)                                                                                             gcggcgGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGcc                                       <  1:501751/139‑1 (MQ=255)                                                                                             gcggcgGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGcc                                       <  2:765247/139‑1 (MQ=255)                                                                                              cggcggCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                                        <  1:825778/57‑1 (MQ=255)                                                                                              cggcggCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                                        >  2:825778/1‑57 (MQ=255)                                                                                              cggcggCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGAt                                                                         <  1:1106082/104‑1 (MQ=255)                                                                                              cggcggCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGAt                                                                         >  2:1106082/1‑104 (MQ=255)                                                                                                 cggcTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                                     >  2:315736/1‑89 (MQ=255)                                                                                                 cggcTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                                     <  1:315736/89‑1 (MQ=255)                                                                                                   gcTGTCCAGGTCTGGTGTCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                                             >  2:1492262/1‑47 (MQ=38)                                                                                                   gcTGTCCAGGTCTGGTGTCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                                             <  1:1492262/47‑1 (MQ=38)                                                                                                   gcTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATaa                                                                                                               >  2:1200411/1‑61 (MQ=255)                                                                                                   gcTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATaa                                                                                                               <  1:1200411/61‑1 (MQ=255)                                                                                                   gcTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTAtt                                                                                         >  2:1206783/1‑83 (MQ=255)                                                                                                   gcTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTAtt                                                                                         <  1:1206783/83‑1 (MQ=255)                                                                                                     tGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTa                                                                                        >  2:398390/1‑82 (MQ=255)                                                                                                     tGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTa                                                                                        <  1:398390/82‑1 (MQ=255)                                                                                                        ccAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCAAACTGCTGGc                            <  1:55395/139‑1 (MQ=255)                                                                                                           ggTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTAt                                                                                          >  1:113319/1‑74 (MQ=255)                                                                                                           ggTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTAt                                                                                          <  2:113319/74‑1 (MQ=255)                                                                                                                 gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATggg                                                                                                                         >  2:209696/1‑37 (MQ=255)                                                                                                                 gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATggg                                                                                                                         <  1:209696/37‑1 (MQ=255)                                                                                                                 gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTa                                                                                        <  2:752436/70‑1 (MQ=255)                                                                                                                 gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTa                                                                                        >  1:752436/1‑70 (MQ=255)                                                                                                                 gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                                     <  2:366955/73‑1 (MQ=255)                                                                                                                 gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                                     >  1:366955/1‑73 (MQ=255)                                                                                                                 gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCa                                                                                   <  2:308733/75‑1 (MQ=255)                                                                                                                 gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCa                                                                                   >  1:308733/1‑75 (MQ=255)                                                                                                                 gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATg                                                                           <  1:898463/83‑1 (MQ=255)                                                                                                                 gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATg                                                                           >  2:898463/1‑83 (MQ=255)                                                                                                                 gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATc                                                                        <  2:1716023/86‑1 (MQ=255)                                                                                                                 gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATc                                                                        >  1:1716023/1‑86 (MQ=255)                                                                                                                  tGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCAAACTGCTGGCAAATCAGgaa                  <  1:576499/139‑1 (MQ=255)                                                                                                                      tttGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCa                                                                                   >  1:76049/1‑70 (MQ=255)                                                                                                                      tttGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCa                                                                                   <  2:76049/70‑1 (MQ=255)                                                                                                                              aCCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCa                                                                                   >  1:31569/1‑62 (MQ=255)                                                                                                                              aCCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCa                                                                                   <  2:31569/62‑1 (MQ=255)                                                                                                                                  tGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                                     >  1:727311/1‑56 (MQ=255)                                                                                                                                  tGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                                     <  2:727311/56‑1 (MQ=255)                                                                                                                                  tGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCAAACTGCTGGCAAATCAGGAAGAAGGGACACAAATcc  >  2:797571/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                       CCGTGCAAGGTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAGAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCAAACTGCTGGCAAATCAGGAAGAAGGGACACAAATCC  >  NC_000913/3545432‑3545699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑