Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,587,370	C→T	T143I (ACT→ATT)  *248* (TAG→TAA)	yhhA → ugpQ ←	DUF2756 family protein glycerophosphodiester phosphodiesterase, cytosolic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,587,370	0	C	T	100.0%	123.9 / NA	40	T143I (ACT→ATT)  *248* (TAG→TAA)	yhhA ugpQ	DUF2756 family protein glycerophosphodiester phosphodiesterase, cytosolic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/22);  total (18/22)

AACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCT  >  NC_000913/3587234‑3587502                                                                                                                                         |                                                                                                                                     aaCACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTa                                                                                                                                    <  1:1293501/139‑1 (MQ=255)           gcggGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTa                                                                                                                          <  2:797333/139‑1 (MQ=255)           gcggGATGTTAAACAGCAACCGTAATCCGGATAGTGCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTa                                                                                                                          <  2:537035/139‑1 (MQ=255)                           cAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCa                                                                                                          >  2:950723/1‑139 (MQ=255)                             aCCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACg                                                                                                        >  2:1270998/1‑139 (MQ=255)                                     ccGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGc                                                                                                <  2:1218790/139‑1 (MQ=255)                                             ttCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTAc                                                                                        >  1:709940/1‑139 (MQ=255)                                               cGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAg                                                                                      >  1:549735/1‑139 (MQ=255)                                                      tCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAAt                                                                               >  2:1023074/1‑139 (MQ=255)                                                               tatGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACAcccc                                                                      >  2:414528/1‑139 (MQ=255)                                                                      ccGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCgcag                                                               <  1:950723/139‑1 (MQ=255)                                                                       cGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAattatt                                                                                                                                  >  2:512214/1‑71 (MQ=255)                                                                       cGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAattatt                                                                                                                                  <  1:512214/71‑1 (MQ=255)                                                                           gaggagaAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAAct                                                          <  2:549735/139‑1 (MQ=255)                                                                                    cGGCGACATGCTGAATCAGCCCAGCACTCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTa                                                                                                                          >  2:1035085/1‑66 (MQ=255)                                                                                    cGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTa                                                                                                                          <  1:1035085/66‑1 (MQ=255)                                                                                     ggCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTggg                                                                                                                               >  1:371959/1‑60 (MQ=255)                                                                                     ggCGACATGCTGAAGCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTggg                                                                                                                               <  2:371959/60‑1 (MQ=255)                                                                                      gCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTgcgcgc                                               <  1:414528/139‑1 (MQ=255)                                                                                      gCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTgcgcgc                                               >  1:1591524/1‑139 (MQ=255)                                                                                       cGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCt                                              <  2:1234241/139‑1 (MQ=255)                                                                                            tGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGt                                         >  1:1698685/1‑139 (MQ=255)                                                                                                   tCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                                  <  1:1270998/139‑1 (MQ=255)                                                                                                   tCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                                  <  2:1591524/139‑1 (MQ=255)                                                                                                     aGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACg                                <  1:1625270/139‑1 (MQ=255)                                                                                                       cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTa                                                                                                                          >  1:1396389/1‑47 (MQ=255)                                                                                                       cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTa                                                                                                                          <  2:1396389/47‑1 (MQ=255)                                                                                                       cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTcaga                                                                                                                 <  2:442062/56‑4 (MQ=255)                                                                                                       cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGa                                                                                                                 >  1:442062/1‑56 (MQ=255)                                                                                                       cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCAt                                                                                              >  1:1510207/1‑75 (MQ=255)                                                                                                       cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCAt                                                                                              <  2:1510207/75‑1 (MQ=255)                                                                                                        ccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGta                             <  2:686751/139‑1 (MQ=255)                                                                                                            cACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATaaa                         >  1:370028/1‑139 (MQ=255)                                                                                                                 cgcAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                                  <  1:1245300/125‑1 (MQ=255)                                                                                                                 cgcAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                                  >  2:1245300/1‑125 (MQ=255)                                                                                                                         gACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAg            >  2:1276973/1‑139 (MQ=255)                                                                                                                              tCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCgg       <  1:1276973/139‑1 (MQ=255)                                                                                                                                cGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCgcag                                                               <  2:627824/81‑1 (MQ=255)                                                                                                                                cGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCgcag                                                               >  1:627824/1‑81 (MQ=255)                                                                                                                                  ttGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                                  >  1:805746/1‑108 (MQ=255)                                                                                                                                  ttGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                                  <  2:805746/108‑1 (MQ=255)                                                                                                                                   tGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCt  <  2:679313/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     AACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCT  >  NC_000913/3587234‑3587502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑